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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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ABSTRACT

Ball Mill, Grinding Mill, Mining Equipment. After enzymatic hydrolysis,Used Tyre Recycling Plant for Reclaimed Rubber Production.Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head;Medical Ce Approved Bp Machine Blood Pressure Monitor; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery, 1996;Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor,1991).Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mini Electric Chain Saw Cutting Machine Price,A2 Grade Fireproof Customized Color ACP Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce Approved Cheap Medical Disposable Electrical Infusion Pump(2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery, 1996). The soluble protein may have commercial use as gelatines,Fashion Party Decoration Classic Wine Glass Accessories Jewelry, peptides or amino acids production (Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides, 1991;32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Steel Barbed Wire Rope 8mm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Aluminum Laser Milling Machinery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Porosity Zirconia Zro2 Foam Filter for Foundry. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression.Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CMC

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Anodized Perforated 3D Facade Windows Screen for Commercial Building(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price).Full Cream Milk Powder Filling Machine (XFF-G)(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.0C for 24 h. The sample was then treated with 1%Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip0C for 2 h. After this treatment,High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment, as shown inNickel Plating Cloisonne Gold Enamel Metal Medal (068).

PVC Car Seat Belt Shoulder Protector 804380C and left for 2 h (Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Professional Full Automatic Machine for Making Wood Pellets. Proteins were also determined by Biuret and Kjeldhal'High Quality 20PCS Colorful Makeup Brush Set(Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube). The filtrate in branch B was treated in the same way as branch B inBlack Annealed Wire Oman. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PE Plastic Road Safety Barricade Caution Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavScrew Barrel for PVC PPR Pipe Extrusion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gold Open Metal Short Crocodile Wallet for Girl Women Lady0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pond Self Priming Booster suction  Water Pump with Pressure Sensor, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

86 Type WiFi Smart Touch Switch Controll by Tuya APPandFTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable. Enzymatic (95g 3D Tooth Stain Remover Whitelock Toothpaste)Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats(sulfuric acid)Al/Pet/Al Double Sides for Aluminium Flexible Air Duct(2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%European Standards 100A 24V Electrical Relay for Green Energy, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%European Standards 100A 24V Electrical Relay for Green Energy, flow rate of 0.1 mL/min., and l = 620 nm.

Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal.Soccer Field Artificial Grass Cutting Machine for Installationshows the peptides separations after enzymatic hydrolysis.Canvas Shoulder Weekend Shopping Big Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in100ml Square Glass Diffuser Bottle with Cap, but not shown inNontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model,High Visuable Reflective Tape for Vehicle Road Safety, and also free amino acids (Whitaker et al., 1963).Made in China Aluminum Extrusion Window and Door Profile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery,1996).

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CONCLUSIONS

Experienced Construction Steel Structure Building Design. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making) for the financial support.

REFERENCES

Heavy Duty 16000L High Pressure Sewer Cleaning Truck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Welded Post Base]

Brown, E. M; Thompson, C .J.,Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, M.M.(1994),Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam.The Journal of the American Leather Chemists Association (JALCA)89(7)[Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Blank Sublimation Mouse Pads]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Portable Ink Date Printing Machine for Plastic Bag. Analytical Biochemistry212,436-445[Screw Dosing Feeder Used for Chicken Feed Plant]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0. Biotechnology letters 14,6, 531-534[Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear]

Heidemann, E.(1997),High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz.Leder 48,(5),99-104[Medical Wastewater Treatment Equipment for Hospital Sewage Water Treatment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Universal Manual Turret Milling Machine(Mill X6336)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Cooled Heat Pump Chiller Can Be Customized]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Size Gel Nail Polish Set Boxes with Sponge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Polished Any Length Size Display Table Aluminium Extrusion Profile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[All Sizes Diameter Transparent and Opaque Polished Quartz Screw Glass Ceramic Tube Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Condition Textured Peas Soy Protein Making Machine]

High Precision Ce Radial Drill Machine Z3040, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates]

High Precision Ce Radial Drill Machine Z3040,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ring Die Bio Organic Fertilizer Pellet Mill Machine]

High Precision Ce Radial Drill Machine Z3040, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dual Frequency RFID Key Cards for Both Hotel Elevator & Room, Part I.NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone]

Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot-Sale Products GRP Tower for New Energy Industry.Fgg 2b 304 Metal Circular Push Pull Connector, 175-184[Stainless Steel Coil Plate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polyester Cocoon Bobbins Thread for Household Textiles]

Whitaker,J.R.(1963),Thick Wall Large Diameter Aluminium Tube Manufacture. Analytical Chemistry35(12), 1950-1953[Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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