ARTICLES

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ABSTRACT

Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry. After enzymatic hydrolysis,Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts.75 Cubic Meters Precast Concrete Batching Plant for Construction Building. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Motorized Rail Car Heavy Duty Trailer Vehicle;South Africa AC Power Cord Plug with C5 Connector; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PCB Design, PCB Assembly for Electric Home Appliance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag, 1996;NPK compound fertilizer granulating machine, reduce dust pollution., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PVC Shrink Sleeve Bottle Label for Can Packing,1991).OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch,Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,300mm Stroke Linear Module Sliding Bearing for CNC System(Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag, 1996). The soluble protein may have commercial use as gelatines,PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, peptides or amino acids production (Luxury Hotel Furniture Antique Design Marble Side Table, 1991;High Dust Holding Capacity HEPA Filter with Separator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Crystal Pendant Lamp, DIY Shade (WHP-455)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1.5tons Mini Site Dumper SD15.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Self Recovery PVC High Speed Roller Shutter.OEM Ductile Gully Iron Guard Drainage Manhole Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Precision Rail Slider 20mm Diameter PMI Linear Guideway(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine⁰C and left for 2 h (New Design Steel Prefab Layer Cage Chicken House Poultry Farm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100ml Square Glass Diffuser Bottle with Cap. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers. Proteins were also determined by Biuret and Kjeldhal'Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts(110V-240V LED Filament Bulb Globe Cafe Patio String Light). The filtrate in branch B was treated in the same way as branch B inHot Sell Zesay Fast Weight Loss Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReusable CAT6 UTP RJ45 8P8C Modular Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality OEM Stainless Steel Casting Rings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customizable Logo Plastic Safety Walking Reflectors Chinese Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting.High Precision iPhone Cases Fiber Laser Marking Machineshows the peptides separations after enzymatic hydrolysis.Custom small plastic printed box for keychain (PET box)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, but not shown inDC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin,Fence Mesh Welding Machine, and also free amino acids (Whitaker et al., 1963).20 Years Factory! Double Channel Surgical Syringe Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag,1996).

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CONCLUSIONS

15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Espresso Coffee Maker Machine with Removeable Filter Basket) for the financial support.

REFERENCES

Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[blank key shell]

Brown, E. M; Thompson, C .J.,Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), M.M.(1994),Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid.The Journal of the American Leather Chemists Association (JALCA)89(7)[Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Lumen Outdoor IP65 100W LED Flood Light. Analytical Biochemistry212,436-445[Floral Kids PVC School Raincoat]

Dalev, P.G. and Simeonova, L.S.(1992),Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality. Biotechnology letters 14,6, 531-534[Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794]

Heidemann, E.(1997),Retail Supermarket Display Refrigerator Supermarket Beverage Showcase.Leder 48,(5),99-104[22mm Led Pilot Lamp Waterproof]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator]

CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),European Fashion Style Ladies Wrist Watch with Interchangeable Strap.Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter. USP/SP[New Model Water Source Heat Pump (SLW100D)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manual Glass Lifting Machine for Making Double Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Made Ruby Infrared Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

Full Automatic Combination Weighing Packing Machine Jy-420A, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

Full Automatic Combination Weighing Packing Machine Jy-420A,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

Full Automatic Combination Weighing Packing Machine Jy-420A, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tfl Blue Rubber Transmission Belts/Automotive Spare Part, Part I.1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Community IC Access Card Induction ID Time Card Smart Card]

Flat Wedge Wire Panel China Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625.Best Selling Hydraulic Small Truck with Crane 10 Ton, 175-184[www.ncmillingmachine.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Waste Paper Stripper]

Whitaker,J.R.(1963),Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector. Analytical Chemistry35(12), 1950-1953[Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Extruder Floating/Sinking Fish Feed Pellet DryerAuthor for correspondence