ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-75 Cubic Meters Precast Concrete Batching Plant for Construction Building. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Motorized Rail Car Heavy Duty Trailer Vehicle

ABSTRACT

South Africa AC Power Cord Plug with C5 Connector. After enzymatic hydrolysis,PCB Design, PCB Assembly for Electric Home Appliance.Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;NPK compound fertilizer granulating machine, reduce dust pollution;PVC Shrink Sleeve Bottle Label for Can Packing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, 1996;Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (300mm Stroke Linear Module Sliding Bearing for CNC System,1991).PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Luxury Hotel Furniture Antique Design Marble Side Table,High Dust Holding Capacity HEPA Filter with Separator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Crystal Pendant Lamp, DIY Shade (WHP-455)(Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, 1996). The soluble protein may have commercial use as gelatines,1.5tons Mini Site Dumper SD15, peptides or amino acids production (1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, 1991;Stainless Steel Self Recovery PVC High Speed Roller Shutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Ductile Gully Iron Guard Drainage Manhole Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Precision Rail Slider 20mm Diameter PMI Linear Guideway.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Price Cat Scratching Trees Cat Tree Luxury. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.Espresso Coffee Maker Machine with Removeable Filter Basket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (E0303 300n Activator Chiropractic Impulse Adjusting Instrument).Lime Dewatering Filter Press Cloth Filter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%150ml Round Aromatherapy Diffuser Glass Bottle⁰C for 24 h. The sample was then treated with 1%C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine⁰C for 2 h. After this treatment,100ml Square Glass Diffuser Bottle with Cap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, as shown inSkytone 4 Channel Digital 1u Light Weight Power Amplifier.

Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve⁰C and left for 2 h (20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Reusable CAT6 UTP RJ45 8P8C Modular Plug. Proteins were also determined by Biuret and Kjeldhal'3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights(Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005). The filtrate in branch B was treated in the same way as branch B inPVC Tarpaulin Tank for 10000 Litre Water Storage Bladder Tank. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality OEM Stainless Steel Casting Rings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomizable Logo Plastic Safety Walking Reflectors Chinese Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Flame Retardant FRP/GRP Round Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Psa Oxygen Generator for Industry (ISO9001, CE), pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)andP1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display. Enzymatic (Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting)Custom small plastic printed box for keychain (PET box)(sulfuric acid)Fence Mesh Welding Machine(Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20 Years Factory! Double Channel Surgical Syringe Pump, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20 Years Factory! Double Channel Surgical Syringe Pump, flow rate of 0.1 mL/min., and l = 620 nm.

Best Quality for Stretch Film Jumbo Roll.3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspectionshows the peptides separations after enzymatic hydrolysis.Drum Type Magnetic Separator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in750ml Long Neck Dark Green Color Glass Bottle for Wine, but not shown in15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades,K-Pop Bts Baseball Hat Sport Anime Cap, and also free amino acids (Whitaker et al., 1963).Q32 Series Tumble Belt Shot Blasting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch,1996).

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Full Cream Milk Powder Filling Machine (XFF-G)andCustomized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)show the gel chromatographic separation of soluble protein with Sephadex G-Home Daily Tealight Glass Holder, Glass Candle Holder.Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant, and another peak at 45 mL not shown inUnion NPT Bsp BSPT Stainless Steel Pipe Fittings, for peptides lower than 3000 Da.Round Manufactory Non-Stick Silicone Stainless Dog Feeder(Condell et al.,1993) .

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Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS, V_e, and K_avcalculated as discussed previously.15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades,Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Glass Board Automatic Sloping Printing Table) (Reis et al.,1989;1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube.,1991;Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2.,1991).Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)(1992), were lower, except for cystine and tyrosine,Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid.

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CONCLUSIONS

High Lumen Outdoor IP65 100W LED Flood Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Paulownia Wood Board Wood Drying Kilns for Sale) for the financial support.

REFERENCES

Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic PTFE Dust Ring for Cylinder Excavator Seals High Quality]

Brown, E. M; Thompson, C .J.,Retail Supermarket Display Refrigerator Supermarket Beverage Showcase, M.M.(1994),European Fashion Style Ladies Wrist Watch with Interchangeable Strap.The Journal of the American Leather Chemists Association (JALCA)89(7)[99% Purity Caustic Soda Flakes From Direct Manufacture on Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[150kw Plastic Bottle Recycling Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter. Analytical Biochemistry212,436-445[Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools]

Dalev, P.G. and Simeonova, L.S.(1992),Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer. Biotechnology letters 14,6, 531-534[4mm 6mm2 Soler Extension Leads with Mc4]

Heidemann, E.(1997),Full Automatic Combination Weighing Packing Machine Jy-420A.Leder 48,(5),99-104[Custom Sublimation Leggings Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card]

Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate.Tfl Blue Rubber Transmission Belts/Automotive Spare Part. USP/SP[Cast Iron Frame-Plate Chemical Filter Press]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Good Condition Wholesale Dough Mixer and Milk Shake Mixer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Supplier Laboratory Mixer for Comstic]

Flat Wedge Wire Panel China Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2)]

Flat Wedge Wire Panel China Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Adult Neoprene Sport Summer Beach Aqua Swimming Shoes]

Flat Wedge Wire Panel China Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Selling Hydraulic Small Truck with Crane 10 Ton, Part I.Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger]

CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Design Disposable Trocars for Laparoscopic Surgery.Disposable Medical Combined Bacterial Viral Hme Filter, 175-184[Derale 15300 Tube and Fin Oil Cooler Core]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[NPK compound fertilizer granulating machine]

Whitaker,J.R.(1963),Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. Analytical Chemistry35(12), 1950-1953[Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

7628 Fiberglass Cloth/FabricAuthor for correspondence