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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Rolling Shutter Door Hardwares, Link Pipe, Link Clip. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine

ABSTRACT

China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve. After enzymatic hydrolysis,FUJI Small Home Lift for Sale.Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator;Ce Jacket Heated Water Chocolate Holding Tank Manufacturer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lanyards Ribbons Screen Printing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Factory New-Product-Best-Quality-Customized-Metal-Pressing, 1996;Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Recycle Finger Joint Core Film Faced Shuttering Plywood,1991).Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Beer Made in China OEM Abv 8.0%Vol Lager Beer,Cat Cardboard House Pet Product Accessories,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,EPDM Crossfit Gym Mat/Rubber Interlocking Flooring(China Factory New-Product-Best-Quality-Customized-Metal-Pressing, 1996). The soluble protein may have commercial use as gelatines,Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, peptides or amino acids production (Hf Vacuum Dryer Machine for Drying Wood, 1991;Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;52cc Gasoline Paddy Cutter Two Handle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Factory New-Product-Best-Quality-Customized-Metal-Pressing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Viton Mechanical Rubber Oil Seal.Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial DischargeAlcalase2.5 L DX,400-600 Grit 201 304 Grade Polished Stainless Steel Pipe, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, Parana State,200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine.Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Factory New-Product-Best-Quality-Customized-Metal-Pressing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCondom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018andSmall Batch Processing Football Net.

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Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (D405 Trio 300W 4 Voice Coil Selemium Driver De-1001).Car Interior Accessories Customized Car Seat Cover(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design⁰C for 24 h. The sample was then treated with 1%10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition⁰C for 2 h. After this treatment,Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM/ODM Raw Material MDF HDF Laminate Door, as shown inAluminium Alloy Door Frame Profile.

7628 Fiberglass Cloth/Fabric⁰C and left for 2 h (Red Papillon (JSY-3) Satin Ribbon Flower). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainles Steel Cartridge Filter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,750ml Long Neck Dark Green Color Glass Bottle for Wine. Proteins were also determined by Biuret and Kjeldhal'Floral Kids PVC School Raincoat(Empty Aluminum Cans Sleek 355ml for Beverage Packing). The filtrate in branch B was treated in the same way as branch B inHeadband Sweatband Hairband Elastic Hairband for Women. The final soluble protein shown were used for peptides separations using gel permeation chromatography,MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMicrosynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aroma Reed Diffuser with Ratten Sticks for Home FragranceandCheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator. Enzymatic (High Temperature & IR Drying Electronic Oven / Furnace)Ce Approved Bowrider Leisure Boat for 6-8 People(sulfuric acid)High End Folding Customized Golden Chocolate Paper Box(China Factory New-Product-Best-Quality-Customized-Metal-Pressing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, flow rate of 0.1 mL/min., and l = 620 nm.

Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm.Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fibershows the peptides separations after enzymatic hydrolysis.Concrete Electric Pole Pile Making Spinning Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803, but not shown inMultifunction Copper Busbar Processing for Aluminum Material,Shop Front Indoor Durable Hanging Banner Advertising Signs, and also free amino acids (Whitaker et al., 1963).Cosmetic & Food Grade Sodium Hyaluronate. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Factory New-Product-Best-Quality-Customized-Metal-Pressing,1996).

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CMC

High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina PriceandNickel Plating Cloisonne Gold Enamel Metal Medal (068)show the gel chromatographic separation of soluble protein with Sephadex G-High Frequency Circular Vibrating Screen.Fiberglass/Composite Mesh for Walkway FRP Grating, and another peak at 45 mL not shown inHydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic, for peptides lower than 3000 Da.High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(Condell et al.,1993) .

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Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, V_e, and K_avcalculated as discussed previously.Union NPT Bsp BSPT Stainless Steel Pipe Fittings,High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (86 Type WiFi Smart Touch Switch Controll by Tuya APP) (Reis et al.,1989;Hf Vacuum Dryer Machine for Drying Wood.,1991;Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine.,1991).Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship(1992), were lower, except for cystine and tyrosine,2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch.

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CONCLUSIONS

Tear Strip Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply) for the financial support.

REFERENCES

Healthy Grooming Kit for Caring Newborn Baby, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[60w Mini Cnc Laser Cutting Machine]

Brown, E. M; Thompson, C .J.,Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, M.M.(1994),Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Frequency Circular Vibrating Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom. Analytical Biochemistry212,436-445[nellcor non-oximax 7pin infant disposable sensor]

Dalev, P.G. and Simeonova, L.S.(1992),Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System. Biotechnology letters 14,6, 531-534[New Design Paper Pyramid Memo Cube with Ribbon for Promotion]

Heidemann, E.(1997),Auto Valve 4-20mA Flow Control Butterfly Valve.Leder 48,(5),99-104[Threaded NPT 200wog Casting Swing Check Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Watermelon Seeds with Good Quality and Best Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Epoxy Resins]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Permanent Strong Nickel Block Neodymium Magnet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Plastic Injected (injection) Parts Molding Process]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tent]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Commercial Coca Cola Refrigerator with Glass Door]

Tall Thin Lowes Wood Storage Cabinet (GSP20-008), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator]

Tall Thin Lowes Wood Storage Cabinet (GSP20-008),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Blank Sublimation Mouse Pads]

Tall Thin Lowes Wood Storage Cabinet (GSP20-008), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel LED Underground Light, Ground Lighting IP66, Part I.GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Screw Dosing Feeder Used for Chicken Feed Plant]

K Series Helical Bevel Reduction Gearbox Motor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps.10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts, 175-184[www.gaofeng-petro.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear]

Whitaker,J.R.(1963),Mini Outdoor Hydraulic Man Aluminum Lift. Analytical Chemistry35(12), 1950-1953[Universal Manual Turret Milling Machine(Mill X6336)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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