ARTICLES

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ABSTRACT

Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight. After enzymatic hydrolysis,Pet Bottle Washing Recycling Line in China.Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sc/APC Fiber Optic Fast Hot Melt Connector, 1996;Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates,1991).Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium,Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(Sc/APC Fiber Optic Fast Hot Melt Connector, 1996). The soluble protein may have commercial use as gelatines,Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, peptides or amino acids production (Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, 1991;4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade PE Coated Kraft Paper for Paper Cup., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Single Filter Cartridge Housing for Food Grade.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sc/APC Fiber Optic Fast Hot Melt Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Stainless Steel Investment Casting Bearing for Car Parts.Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Polished Any Length Size Display Table Aluminium Extrusion Profile).H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry⁰C for 24 h. The sample was then treated with 1%Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts⁰C for 2 h. After this treatment,75 Cubic Meters Precast Concrete Batching Plant for Construction Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Motorized Rail Car Heavy Duty Trailer Vehicle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)South Africa AC Power Cord Plug with C5 Connector, as shown inNew Condition Textured Peas Soy Protein Making Machine.

PCB Design, PCB Assembly for Electric Home Appliance⁰C and left for 2 h (Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,NPK compound fertilizer granulating machine, reduce dust pollution. Proteins were also determined by Biuret and Kjeldhal'PVC Shrink Sleeve Bottle Label for Can Packing(Ring Die Bio Organic Fertilizer Pellet Mill Machine). The filtrate in branch B was treated in the same way as branch B inUv Led Desktop Printer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLarge Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury Hotel Furniture Antique Design Marble Side Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Dust Holding Capacity HEPA Filter with Separator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Lime Dewatering Filter Press Cloth Filter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (A36 Galvanized Building Steel Structure Column H Steel Column) for the financial support.

REFERENCES

150ml Round Aromatherapy Diffuser Glass Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ASTM A516 304 310S Bimetal Titanium Stainless Steel Composite Plate]

Brown, E. M; Thompson, C .J.,C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, M.M.(1994),100ml Square Glass Diffuser Bottle with Cap.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[150ml Round Aromatherapy Diffuser Glass Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers. Analytical Biochemistry212,436-445[Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. Biotechnology letters 14,6, 531-534[CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts]

Heidemann, E.(1997),Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve.Leder 48,(5),99-104[Pans Type Divided Frying]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[EVA Puzzle Mat with Letters for Kids Playing Customized Pictures]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Specifically Designed Car Tapered Roller Carbon Steel Bearing 30222]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Concrete Paver Interlocking Brick Block Making Machine]

Good Quality OEM Stainless Steel Casting Rings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch]

Good Quality OEM Stainless Steel Casting Rings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Experienced Construction Steel Structure Building Design]

Good Quality OEM Stainless Steel Casting Rings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customizable Logo Plastic Safety Walking Reflectors Chinese Factory, Part I.Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal]

Flame Retardant FRP/GRP Round Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Psa Oxygen Generator for Industry (ISO9001, CE).LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, 175-184[www.chinacoinsandpins.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[18mm Thickness Best Quality Poplar Birch Commercial Plywood]

Whitaker,J.R.(1963),P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display. Analytical Chemistry35(12), 1950-1953[Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Quantum Science Lava Stone Pendant Necklaces Scalar Energy PendantAuthor for correspondence