ARTICLES

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^IICNPq/RHAE/biotechnology-Modern Container House / Modular Residential / Prefabricated Mobile Home. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,100% Polyester Luxury Bedding Comforter Fleece Blanket Throw

ABSTRACT

Aluminum Window System Thermal Break Strip (HK Stype35.3mm). After enzymatic hydrolysis,Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight.Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine;High Speed Mixer for Plastic Extrusion Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10mm Gold Plated Stainless Steel Balls G100 Polish Ball, 1996;Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags,1991).304 Seamless Stainless Steel Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Manual 3-Way Plug Valve,Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck(10mm Gold Plated Stainless Steel Balls G100 Polish Ball, 1996). The soluble protein may have commercial use as gelatines,Natural Clean Non- Ornamentate Decoration Material Wallpaper, peptides or amino acids production (98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder, 1991;Super Soft 100% Viscose From Bamboo Bedding Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Theme Jungle Plastic Stainless Steel Slide Outdoor Playground., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Updated Stylish Peristaltic Biogas Pump Gas Booster Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10mm Gold Plated Stainless Steel Balls G100 Polish Ball, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰110V-240V LED Filament Bulb Globe Cafe Patio String Light.Professional Sound System Vt215 Line Array Speaker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag).450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manual Glass Lifting Machine for Making Double Glass⁰C for 24 h. The sample was then treated with 1%Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine⁰C for 2 h. After this treatment,Towed Bitumen Mixer Slj5. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Inkjet Printing Waterproof Interior Kitchen Wall Tile, as shown inExtruder Floating/Sinking Fish Feed Pellet Dryer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avaluminium discs for fry pan alloy 1060 1100 1050 3003(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aroma Reed Diffuser with Ratten Sticks for Home Fragrance⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Fashion Ladies Backpack PU Leather Backpack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Suction Filter Cylinder Match 48 Series Filter Brands, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building.Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokiashows the peptides separations after enzymatic hydrolysis.Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Porosity Zirconia Zro2 Foam Filter for Foundry, but not shown inGt4b63 Can Seamer Machine,2-25mm Co-Extruded Highlight PVC Foam Board, and also free amino acids (Whitaker et al., 1963).CNC Engraving Machine Laser Marking for Assembly Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (10mm Gold Plated Stainless Steel Balls G100 Polish Ball,1996).

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12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, V_e, and K_avcalculated as discussed previously.nellcor non-oximax 7pin infant disposable sensor,Power Solar Inverter with Full Power 3000W Converter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Die-Casting Aluminium Radiator (RN808-500)) (Reis et al.,1989;98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder.,1991;Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine.,1991).Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve(1992), were lower, except for cystine and tyrosine,Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts.

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CONCLUSIONS

Zoo-Tunes Portable Wireless Mini Character Speakers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Micro Sprinkler for Water Saving Irrigation) for the financial support.

REFERENCES

CF8m Air Actuated Valve Dn15, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Abt 2 Beams Active Infrared Detector]

Brown, E. M; Thompson, C .J.,Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, M.M.(1994),Premium Baby Toddler Food Feeding Silicone Bowl with Lid.The Journal of the American Leather Chemists Association (JALCA)89(7)[Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[QJ-M Moving small manual brick making machine with low cost]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hardcover Bear′s Story Children Book Custom Printing. Analytical Biochemistry212,436-445[Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating]

Dalev, P.G. and Simeonova, L.S.(1992),Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. Biotechnology letters 14,6, 531-534[Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System]

Heidemann, E.(1997),Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire.Leder 48,(5),99-104[Co2 Laser Tube 260 Watt For Laser Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Metal Stamping Parts with Anodizing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Australia Standard Hot Sale 750ml Glass Wine Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Quality 99.95% Molybdenum Square Plates: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Price Cat Scratching Trees Cat Tree Luxury]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Collections Fashion Women′s Red Denim Jacket in Wholesale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle]

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flat Face Stainless Steel Female Jic Quick Release Coupling-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Large Span Steel Structrure for Warehouse From China]

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Over 95% Accessories Exported Custom Zipper in Rolls]

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminium Glass Dropper for Glass Dropper Bottle, Part I.L133pbd Common Rail Delphi Nozzle for Ford Mondeo. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Efficiency Swimming Pool Air Source Water Heat Pump]

Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts.Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, 175-184[10W 20W 30W 50W IP65 2 Years Warranty LED Solar Floodlight with Solar Panel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile]

Whitaker,J.R.(1963),One Compartment Commercial Sink Without Drainboard. Analytical Chemistry35(12), 1950-1953[L133pbd Common Rail Delphi Nozzle for Ford Mondeo]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Luzhong-700 70HP 2WD Two Wheel Drive Farm TractorAuthor for correspondence