ARTICLES

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ABSTRACT

Injection Mold Maker for Plastic Fighter Toy. After enzymatic hydrolysis,PCBA SMT Equipment PCB Depaneling Machine.HDPE Pipe for Water Supply (OD: 16mm-800mm). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Digital Floating Baby Care Products Frog Bath Design Thermometer, 1996;Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board,1991).FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Colorful Asphalt Shingle for Roof Water Proof,Durable Inline Hockey Skate Puck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane)(Digital Floating Baby Care Products Frog Bath Design Thermometer, 1996). The soluble protein may have commercial use as gelatines,18mm Thickness Best Quality Poplar Birch Commercial Plywood, peptides or amino acids production (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1991;Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nylon Hammer Fixings Drive Anchor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Electrolyte Effervescent Tablet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Digital Floating Baby Care Products Frog Bath Design Thermometer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter.Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

304 321 310 310S Welded Decorative Stainless Steel PipeAlcalase2.5 L DX,Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Manual Electric Winch Small Winch 220V Winch); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar, Parana State,Auto Water Cooling Radiator Release Temperature Screw Air Compressor.Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Digital Floating Baby Care Products Frog Bath Design Thermometer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC HandrailandPlastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A.

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Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Reinforced Brass Coated Steel Wire for Hose Wire).Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Plate Fin Heat Exchanger/Oil Cooler Core⁰C for 24 h. The sample was then treated with 1%3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection⁰C for 2 h. After this treatment,3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5-Axis CNC Machining Billet Aluminum Engine Rotary Housing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle, as shown inPE Pipe in SDR 11 Pressing Rate for Fire-Control.

Custom Clamshell Book Shape Black Cardboard Magnet Gift Box⁰C and left for 2 h (Mini Electric Chain Saw Cutting Machine Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashionable Simple Printing Mini Key & Coin Purse. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch. Proteins were also determined by Biuret and Kjeldhal'Kids Folding Play Mat Kids Play Room Floor Mat(Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode). The filtrate in branch B was treated in the same way as branch B in15kv to 35kv Conservator Type Transformers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Roller Window Shutter for Residential Usage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pre-Seasoned Cast Iron 5-Piece Cookware Set. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Hose SAE 100 R1AT⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Tamper Evident Branded Printed Packing Pet Adhesive Security Tape.Shop Front Indoor Durable Hanging Banner Advertising Signsshows the peptides separations after enzymatic hydrolysis.530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in304 Stainless Steel Expansion Anchor Bolt, but not shown in2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery,Hotel Use and Steel Material Poultry House for 10000 Chickens, and also free amino acids (Whitaker et al., 1963).50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Digital Floating Baby Care Products Frog Bath Design Thermometer,1996).

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White Electrical Fireplace Warmer, V_e, and K_avcalculated as discussed previously.Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor,Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch) (Reis et al.,1989;European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag.,1991;Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.,1991).4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone(1992), were lower, except for cystine and tyrosine,1 Inch Stainless Steel Flexible Exhaust Pipe.

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CONCLUSIONS

Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manual Electric Winch Small Winch 220V Winch) for the financial support.

REFERENCES

Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Venlo Type Glass Greenhouse Intelligent Greenhouse]

Brown, E. M; Thompson, C .J.,Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator, M.M.(1994),Ce Certificate High Security Steel Shackle Padlock.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini Succulent Planters]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Drive Shaft for Jmc 8-94134458-PT. Analytical Biochemistry212,436-445[Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System]

Dalev, P.G. and Simeonova, L.S.(1992),DSG Ball Screw Fixed Seat Nut Support. Biotechnology letters 14,6, 531-534[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Heidemann, E.(1997),Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car.Leder 48,(5),99-104[Gear Hobbing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric]

Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Apex Digital UV Printers for Aluminum Board Printing UV6090.24 Molds Commercial Ice Cream Cone Maker. USP/SP[Low Price Digital Temperature Controller]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Motar Plastering Machine with Screw Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Square Vibrating Sieve Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disposable Surgical Sterile Skin Stapler with Ce and ISO]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Optical PCB Bar Connector for Jst Scn 2.5mm]

Fashion Children Fur Pompom Beanie Scarf Knit Set, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Steel Jacket Steam Insulation Pipeline Production Best Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glazed Metal Roof Tile Roll Forming Machine with Ce]

Fashion Children Fur Pompom Beanie Scarf Knit Set,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pet Bottle Washing Recycling Line in China]

Fashion Children Fur Pompom Beanie Scarf Knit Set, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Haa 800mAh NiMH Battery Pack, Part I.Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[95g 3D Tooth Stain Remover Whitelock Toothpaste]

Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electronic Muscle Stimulator EMS Machine for Slimming.Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, 175-184[Cnc Router Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors]

Whitaker,J.R.(1963),Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover. Analytical Chemistry35(12), 1950-1953[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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