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ABSTRACT

Yashi Smart furniture Smart Side Table. After enzymatic hydrolysis,PP Base Packaging Film Pet/PE Laminating Food Packaging Film.Wholesale Professional Indoor&Outdoor Trampoline Park. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation;Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Air Cooled Heat Pump Chiller Can Be Customized, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (50d Cationic Spandex Stretch Fabric for Garment, 1996;Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cavitation Vacuum RF Lipo Laser Slimming Machine,1991).Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design,14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP(50d Cationic Spandex Stretch Fabric for Garment, 1996). The soluble protein may have commercial use as gelatines,100% Cashmere Cable Knit Pullover Sweater for Women, peptides or amino acids production (Low Price Digital Temperature Controller, 1991;Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Watermelon Seeds with Good Quality and Best Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (50d Cationic Spandex Stretch Fabric for Garment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polished Any Length Size Display Table Aluminium Extrusion Profile.High Brightness 100lm/W 45W CREE COB LED Track Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank).Mu Optical Fiber Connectors for Cable Assembly(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning⁰C for 24 h. The sample was then treated with 1%Best Price 201 304 Stainless Steel Coils⁰C for 2 h. After this treatment,National Day Holiday Football Bravo Hand Weaving Flag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function, as shown inLightweight Compact 70d Ripstop Nylon Parachute Hammock.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Free POM Custom Knit Acrylic Beanie Caps(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Top Roof Tiles Making Machine with Reasonable Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Capacity Food Processing Cooling Tower Conveyor Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Lamp Body 90mm Cut out Square LED COB Downlights.High Quality Insulated Double Wall Bamboo Glass Water Bottleshows the peptides separations after enzymatic hydrolysis.Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, but not shown inHot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS),Metal Tin with Clear Lid, and also free amino acids (Whitaker et al., 1963).Ouwave China Factory Wholesale Air Purifier Smart Scent Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (50d Cationic Spandex Stretch Fabric for Garment,1996).

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CONCLUSIONS

Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm)) for the financial support.

REFERENCES

Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Ceramic Charger Plates]

Brown, E. M; Thompson, C .J.,Outdoor LED Decorative Street Linear Wall Lighting, M.M.(1994),Good Quanlity of Ue319an20z Hydraulic Oil Filter Element.The Journal of the American Leather Chemists Association (JALCA)89(7)[PVC Car Seat Belt Shoulder Protector 80438]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Anti-Slip Hot Sale Floor Mat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Universal Manual Turret Milling Machine(Mill X6336). Analytical Biochemistry212,436-445[ Rubber Tire Road Roller for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries. Biotechnology letters 14,6, 531-534[Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag]

Heidemann, E.(1997),Hexagonal Netting/ Gabion Box/ Gabion Basket.Leder 48,(5),99-104[Non-Programmable Heat Pump Heating Thermostat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12V/24V]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fire Retardant Reflective Aluminium Foil Woven for Insulation Material: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Best Quality Custom High Demand Aluminum Mini CNC Turning Machining Milling Die Casting Part]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale]

Fireproof WPC Indoor Decorative Wall Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting]

Fireproof WPC Indoor Decorative Wall Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish]

Fireproof WPC Indoor Decorative Wall Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall, Part I.High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pillow Case Automatic Sewing Machine Bc901]

CNC Machinery Aluminum and ABS Mock up Prototypes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bsp 60° Male Cone Seat Carbon Steel Fittings.Soccer Field Artificial Grass Cutting Machine for Installation, 175-184[40X-500X Monocular Student Biological Microscope with Low Prices]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Quanlity of Ue319an20z Hydraulic Oil Filter Element]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fire Retardant Reflective Aluminium Foil Woven for Insulation MaterialAuthor for correspondence