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ABSTRACT

DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws. After enzymatic hydrolysis,Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly.High Speed Automatic Slitting Machine for Plastic Film. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Sheet Metal Parts Custom Fabrication, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip, 1996;Blue Color Tie-on Surgical Face Mask Soft Touch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine,1991).Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Private Customized High Quality Printed Shipping Labels,DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen(Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip, 1996). The soluble protein may have commercial use as gelatines,New Product 90GSM Sublimation Paper for Economic Printing, peptides or amino acids production (1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System, 1991;Color Coated Prepainted PPGI PPGL Steel Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Clear Customized PVC Cosmetic Package Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Professional Design Wing Shape Engraved Car Label Emblem.38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Supplier Dual Voltage Steam Curling Tongs (A129)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine).PE Pipe in SDR 11 Pressing Rate for Fire-Control(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric Automotive Heater Control Valve⁰C for 24 h. The sample was then treated with 1%Shoelace-Free Climbing Shoes with Wire Button⁰C for 2 h. After this treatment,Kraft Paper Laminated Aluminum Foil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, as shown inFactory Wholesale Reach Standard Home and Office Furniture Fitting.

Stainless Steel Watch⁰C and left for 2 h (Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine. Proteins were also determined by Biuret and Kjeldhal'High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine(Custom Silver Anodized Aluminium 135 Degree Angle Bracket). The filtrate in branch B was treated in the same way as branch B inHeavy Duty 90 Degree Large Decorative Table Iron Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Women Luxury Handbags Leather Shoulder Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avP10 3X4m RGB LED Video Curtain for DJ Stage Backdrop(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Long Stroke Hydraulic Cylinder for Constraction Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Neoprene Protective Elbow Protector Brace Elbow Support (NS0012), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Big Smart Plant Hydroponic Planter with Indoor Garden Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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EV Charger for 120KW DC Fast Electric Car Charging Station. Drum Rotatorshows the peptides separations after enzymatic hydrolysis.Custom Velvet Jewelry Box Gift Box with Bowknots for Packing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Lid Press Machine, but not shown inHite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets,Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush, and also free amino acids (Whitaker et al., 1963).Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip,1996).

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CONCLUSIONS

Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle) for the financial support.

REFERENCES

Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom English Thread Adapter]

Brown, E. M; Thompson, C .J.,The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel, M.M.(1994),Die-Casting Aluminium Radiator (RN808-500).The Journal of the American Leather Chemists Association (JALCA)89(7)[Haa 800mAh NiMH Battery Pack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL. Analytical Biochemistry212,436-445[Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Wholecut Oxford Plain Toe Black Antique Men Shoes. Biotechnology letters 14,6, 531-534[CNC Turned Aluminum Anodized Auto Part]

Heidemann, E.(1997),New Technology Carbon Cleaning Tools Auto Accessories.Leder 48,(5),99-104[Floor Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China 100% PU Synthetic Leather for Shoes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Flip Flops PVC EVA PE Rubber Soles Strap Attaching Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti-Abrasion PVC Artificial Leather for Car Seat (128#)]

TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Laser Customized Telescopic Steel Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts]

TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[EV Charger for 120KW DC Fast Electric Car Charging Station]

TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision Mini Laser Cutting System, Part I.Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China]

1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),One to One Order Following Strong Real Gold Thread.New Design Paper Pyramid Memo Cube with Ribbon for Promotion, 175-184[High Quality Australia Modern White Kitchen Pantry Cupboards Kitchen Cabinets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fake Fish Luminescent Tropical Fish Fish Tank Decoration]

Whitaker,J.R.(1963),Plastic Medallion, Plastic Ceiling Rose. Analytical Chemistry35(12), 1950-1953[Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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