ARTICLES

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ABSTRACT

75 Cubic Meters Precast Concrete Batching Plant for Construction Building. After enzymatic hydrolysis,Motorized Rail Car Heavy Duty Trailer Vehicle.South Africa AC Power Cord Plug with C5 Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.NPK compound fertilizer granulating machine, reduce dust pollution, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Shrink Sleeve Bottle Label for Can Packing, 1996;OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch,1991).Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,300mm Stroke Linear Module Sliding Bearing for CNC System,PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Luxury Hotel Furniture Antique Design Marble Side Table(PVC Shrink Sleeve Bottle Label for Can Packing, 1996). The soluble protein may have commercial use as gelatines,High Dust Holding Capacity HEPA Filter with Separator, peptides or amino acids production (Crystal Pendant Lamp, DIY Shade (WHP-455), 1991;1.5tons Mini Site Dumper SD15, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Self Recovery PVC High Speed Roller Shutter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Ductile Gully Iron Guard Drainage Manhole Cover. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Shrink Sleeve Bottle Label for Can Packing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Precision Rail Slider 20mm Diameter PMI Linear Guideway.Factory Price Cat Scratching Trees Cat Tree Luxury-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600).Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies⁰C for 24 h. The sample was then treated with 1%Lime Dewatering Filter Press Cloth Filter⁰C for 2 h. After this treatment,150ml Round Aromatherapy Diffuser Glass Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100ml Square Glass Diffuser Bottle with Cap, as shown inHalogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type).

Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers⁰C and left for 2 h (Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve. Proteins were also determined by Biuret and Kjeldhal'17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS(LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp). The filtrate in branch B was treated in the same way as branch B inHydraulic Oil Buffer Pricelist. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reusable CAT6 UTP RJ45 8P8C Modular Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Quality OEM Stainless Steel Casting Rings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customizable Logo Plastic Safety Walking Reflectors Chinese Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flame Retardant FRP/GRP Round Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fence Mesh Welding Machine.Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Partsshows the peptides separations after enzymatic hydrolysis.20 Years Factory! Double Channel Surgical Syringe Pump(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLight Weight Ohsas 18001 Certified Metal Arch Buildings, but not shown inHigh Performance Alcohol Pad Making Machine,Best Quality for Stretch Film Jumbo Roll, and also free amino acids (Whitaker et al., 1963).Drum Type Magnetic Separator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PVC Shrink Sleeve Bottle Label for Can Packing,1996).

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CONCLUSIONS

Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Profile Sp-Level Linear Rail for 3D Printer) for the financial support.

REFERENCES

Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[16a Bms Pcb For Li-ion/ Lifepo4/ Lipo Battery Pack]

Brown, E. M; Thompson, C .J.,High Lumen Outdoor IP65 100W LED Flood Light, M.M.(1994),Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[HDPE Pipe for Water Supply (OD: 16mm-800mm)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Retail Supermarket Display Refrigerator Supermarket Beverage Showcase. Analytical Biochemistry212,436-445[Small Diameter Coil Compression Spring From Factory]

Dalev, P.G. and Simeonova, L.S.(1992),European Fashion Style Ladies Wrist Watch with Interchangeable Strap. Biotechnology letters 14,6, 531-534[Stainless Steel French Earring Hook for Jewelry]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB]

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer.Full Automatic Combination Weighing Packing Machine Jy-420A. USP/SP[Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ergonomic Revolving Chair Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FTTH Optical Network Terminal for Gpon ONU and CATV]

Tfl Blue Rubber Transmission Belts/Automotive Spare Part, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Automotive Heater Control Valve]

Tfl Blue Rubber Transmission Belts/Automotive Spare Part,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Single Filter Cartridge Housing for Food Grade]

Tfl Blue Rubber Transmission Belts/Automotive Spare Part, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flat Wedge Wire Panel China Factory, Part I.Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor LED Decorative Street Linear Wall Lighting]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart]

Whitaker,J.R.(1963),New Design Disposable Trocars for Laparoscopic Surgery. Analytical Chemistry35(12), 1950-1953[Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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