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ABSTRACT

High Quality Mini LED Flood Light SMD Flood Light 150W. After enzymatic hydrolysis,Low Price Fashion Unique Plastic Zipper.Silica Gel- Indicating Blue to Pink After Adsorption. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine, 1996;Road Safety Reflective Waterproof Plastic Reflectors., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary,1991).High Quality Gas Spring Strut Gas Shock for Wall Bed. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial Bedsheet Folding Machine (ZD-3000),Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM ODM Aluminum Casting Parts(4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine, 1996). The soluble protein may have commercial use as gelatines,High Pressure Low Volume 2900rpm electric Water Pump, peptides or amino acids production (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1991;Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multifunction Copper Busbar Processing for Aluminum Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Men Genuine Leather Sling Shoulder Satchel Messenger Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric DC 24V Geared Motor for Auto Doors.Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:RF Shielding MRI Door with Brass Frame for RF Cage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dubai Products Rectified Tile for Wall(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Grinding Factory Corn Maize Flour Milling Mill Making Machines).ANSI B16.5 Stainless Steel SS316 Forging Blind Flange(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection⁰C for 24 h. The sample was then treated with 1%2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer⁰C for 2 h. After this treatment,Integrated Hydraulic Steering Control Units (BZZ1F SCU). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Model Water Source Heat Pump (SLW100D)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment, as shown inHot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556.

Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube⁰C and left for 2 h (Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325. Proteins were also determined by Biuret and Kjeldhal'High Capacity Vibrating Screen Manufacturer(Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging). The filtrate in branch B was treated in the same way as branch B inHot Selling Dpl/Opt/IPL Machine Light Therapy Hair Removal Pigmentation Treatment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electrochemical Polishing Tungsten Evaporation Materials.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhite UTP Cat5e UK Type Tooless RJ45 Keystone Jack(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheese Puffed Snacks Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainles Steel Cartridge FilterandChina High Profit Automatic Pet Extruder Machine. Enzymatic (Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337))Best Price Spc Lvt PVC Vinyl Flooring for Home Office(sulfuric acid)Red Papillon (JSY-3) Satin Ribbon Flower(4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, flow rate of 0.1 mL/min., and l = 620 nm.

Steel Structure Construction Building Metal House.Bgf-800 Pet Dry Laminating Machineshows the peptides separations after enzymatic hydrolysis.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSteel Structure Construction Building Metal House, but not shown inNew 60W LED Beam Zoom Moving Head with Prism,Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, and also free amino acids (Whitaker et al., 1963).Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine,1996).

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Lab Water Instruments/Lab Water Purification System MicroandProper Price Good Quality 10ml 20ml Airless Pump Bottleshow the gel chromatographic separation of soluble protein with Sephadex G-2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415).New Design Slotted Board MDF for Wall Panel, and another peak at 45 mL not shown inQ37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning, for peptides lower than 3000 Da.Factory Wholesale Reach Standard Home and Office Furniture Fitting(Condell et al.,1993) .

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CONCLUSIONS

Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dental Emax Empress Crown From China Dental Lab) for the financial support.

REFERENCES

Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Protective Suit]

Brown, E. M; Thompson, C .J.,CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101, M.M.(1994),High Quality Portable Polyester Duffel Garment Tote Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Decoration Sign Green Grey White PVC Foam Board. Analytical Biochemistry212,436-445[Light Weight Ohsas 18001 Certified Metal Arch Buildings]

Dalev, P.G. and Simeonova, L.S.(1992),3kw 4kw 5kw off Grid Solar Inverter. Biotechnology letters 14,6, 531-534[High Performance Alcohol Pad Making Machine]

Heidemann, E.(1997),OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch.Leder 48,(5),99-104[Silicone Brush]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet Material Alien 9654 Wet Inlay UHF Windshield Label: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Arriva Pet Dog Cat Nail Toe Claw Clipper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Quality Sanitary Tube Shell Tube Heat Exchanger]

Reinforced Brass Coated Steel Wire for Hose Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Extruded Polystyrene Foam Board Custom Edges S/L]

Reinforced Brass Coated Steel Wire for Hose Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ High Pressure Lay Flat Hose for Industrial Discharge]

Reinforced Brass Coated Steel Wire for Hose Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EPE Foam Package Extruding Production Line, Part I.3 Layer Film Blowing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine]

8FT Wood Log Debarker Machine for Plywood Machinery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine.Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, 175-184[Cast Iron Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HDPE Pipe for Water Supply (OD: 16mm-800mm)]

Whitaker,J.R.(1963),High Density Graphite Crucible for Melting Metal. Analytical Chemistry35(12), 1950-1953[Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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