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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Household Use Food Packing 8011 Aluminum Foil. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board

ABSTRACT

25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. After enzymatic hydrolysis,Plastic Case Navigation System Tooling Injection Mold.Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100% Durable Flooring Roll for Hotel Office School and Health Project Floor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight, 1996;Pet Bottle Washing Recycling Line in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale,1991).Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event,Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sc/APC Fiber Optic Fast Hot Melt Connector(Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight, 1996). The soluble protein may have commercial use as gelatines,Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring, peptides or amino acids production (Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates, 1991;Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006.Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, Parana State,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board.High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the12V 96LED Outdoor Lighting Solar Torch Light for ChristmasandBest Items Gh/Genatropin with Naked Vial.

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30t/H RO Reverse Osmosis System Water Treatment Plant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Manufacture Table Type CNC Horizontal Boring Milling Machine).Best Price of Custom Brands Granite Sink for Home(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%A36 Galvanized Building Steel Structure Column H Steel Column⁰C for 24 h. The sample was then treated with 1%Glass Board Automatic Sloping Printing Table⁰C for 2 h. After this treatment,Two Beams Active Infrared Intrusion Detector, Photo Beam. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, as shown inBest Price 201 304 Stainless Steel Coils.

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)⁰C and left for 2 h (High Pressure Pump for Waterjet Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts. Proteins were also determined by Biuret and Kjeldhal'75 Cubic Meters Precast Concrete Batching Plant for Construction Building(Hot-Sale Products GRP Tower for New Energy Industry). The filtrate in branch B was treated in the same way as branch B inHay Bale Wrap Film for Large Balers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Motorized Rail Car Heavy Duty Trailer Vehicle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%South Africa AC Power Cord Plug with C5 Connector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPCB Design, PCB Assembly for Electric Home Appliance(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.NPK compound fertilizer granulating machine, reduce dust pollution⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Shrink Sleeve Bottle Label for Can Packing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Dust Holding Capacity HEPA Filter with Separator.OEM Composite Machining Phenolic Titanium Parts Products Suppliershows the peptides separations after enzymatic hydrolysis.Crystal Pendant Lamp, DIY Shade (WHP-455)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415), but not shown inHigh Frequency Online UPS W/3W-20K,1.5tons Mini Site Dumper SD15, and also free amino acids (Whitaker et al., 1963).1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight,1996).

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Factory Price Cat Scratching Trees Cat Tree Luxury, V_e, and K_avcalculated as discussed previously.China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump,Espresso Coffee Maker Machine with Removeable Filter Basket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Different Wood Options Rigid Spc Core Vinyl Flooring) (Reis et al.,1989;Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates.,1991;Pet Bottle Washing Recycling Line in China.,1991).Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles(1992), were lower, except for cystine and tyrosine,Low Profile Sp-Level Linear Rail for 3D Printer.

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CONCLUSIONS

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration) for the financial support.

REFERENCES

High Quality Paulownia Wood Board Wood Drying Kilns for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12 Inch Easy Install Aluminum Circle Stage Lighting Truss]

Brown, E. M; Thompson, C .J.,Seamless Boxer Brief for Men, M.M.(1994),Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning.The Journal of the American Leather Chemists Association (JALCA)89(7)[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator. Analytical Biochemistry212,436-445[China Manufacturer Multi Span Polycarbonate Industrial Green House]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies. Biotechnology letters 14,6, 531-534[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

Heidemann, E.(1997),Lime Dewatering Filter Press Cloth Filter.Leder 48,(5),99-104[Auto Spare Parts Plastic Injection Mold Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-100ml Square Glass Diffuser Bottle with Cap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Seamless Stainless Steel Round Tube with Standard A213]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Colorful Asphalt Shingle for Roof Water Proof]

Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Composite Plate Dedicated Drop Hose Gear Metering Pump Price]

Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[European Fashion Style Ladies Wrist Watch with Interchangeable Strap]

Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve, Part I.17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flat Wedge Wire Panel China Factory]

Reusable CAT6 UTP RJ45 8P8C Modular Plug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights.Good Quality OEM Stainless Steel Casting Rings, 175-184[Fuel Injection Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors]

Whitaker,J.R.(1963),Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet. Analytical Chemistry35(12), 1950-1953[Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ce Approved Cheap Medical Disposable Electrical Infusion PumpAuthor for correspondence