ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Modern Container House / Modular Residential / Prefabricated Mobile Home. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,100% Polyester Luxury Bedding Comforter Fleece Blanket Throw

ABSTRACT

Aluminum Window System Thermal Break Strip (HK Stype35.3mm). After enzymatic hydrolysis,Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight.Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine;High Speed Mixer for Plastic Extrusion Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10mm Gold Plated Stainless Steel Balls G100 Polish Ball, 1996;Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags,1991).304 Seamless Stainless Steel Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Manual 3-Way Plug Valve,Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck(10mm Gold Plated Stainless Steel Balls G100 Polish Ball, 1996). The soluble protein may have commercial use as gelatines,Natural Clean Non- Ornamentate Decoration Material Wallpaper, peptides or amino acids production (98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder, 1991;Super Soft 100% Viscose From Bamboo Bedding Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Theme Jungle Plastic Stainless Steel Slide Outdoor Playground., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Updated Stylish Peristaltic Biogas Pump Gas Booster Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10mm Gold Plated Stainless Steel Balls G100 Polish Ball, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰110V-240V LED Filament Bulb Globe Cafe Patio String Light.Professional Sound System Vt215 Line Array Speaker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine).450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manual Glass Lifting Machine for Making Double Glass⁰C for 24 h. The sample was then treated with 1%Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine⁰C for 2 h. After this treatment,Towed Bitumen Mixer Slj5. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Inkjet Printing Waterproof Interior Kitchen Wall Tile, as shown in2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avaluminium discs for fry pan alloy 1060 1100 1050 3003(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aroma Reed Diffuser with Ratten Sticks for Home Fragrance⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Fashion Ladies Backpack PU Leather Backpack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Suction Filter Cylinder Match 48 Series Filter Brands, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, V_e, and K_avcalculated as discussed previously.nellcor non-oximax 7pin infant disposable sensor,Power Solar Inverter with Full Power 3000W Converter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter) (Reis et al.,1989;98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder.,1991;Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine.,1991).Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve(1992), were lower, except for cystine and tyrosine,Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts.

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CONCLUSIONS

Zoo-Tunes Portable Wireless Mini Character Speakers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Micro Sprinkler for Water Saving Irrigation) for the financial support.

REFERENCES

CF8m Air Actuated Valve Dn15, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fly Sticky Board]

Brown, E. M; Thompson, C .J.,Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, M.M.(1994),Premium Baby Toddler Food Feeding Silicone Bowl with Lid.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hardcover Bear′s Story Children Book Custom Printing. Analytical Biochemistry212,436-445[Pillow Case Automatic Sewing Machine Bc901]

Dalev, P.G. and Simeonova, L.S.(1992),Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. Biotechnology letters 14,6, 531-534[Good Quanlity of Ue319an20z Hydraulic Oil Filter Element]

Heidemann, E.(1997),Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire.Leder 48,(5),99-104[Festival Event Safety Access Control Customizable Woven Fabric Cloth RFID Wristband]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Pressure Low Volume 2900rpm electric Water Pump]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cosmetic & Personal Care Grade Xanthan Gum]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Quality 99.95% Molybdenum Square Plates: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Machinery Aluminum and ABS Mock up Prototypes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Horizontal Mini Hydraulic Power Pack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Thick Wall Large Diameter Aluminium Tube Manufacture]

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flat Face Stainless Steel Female Jic Quick Release Coupling-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)]

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing]

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminium Glass Dropper for Glass Dropper Bottle, Part I.L133pbd Common Rail Delphi Nozzle for Ford Mondeo. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Commercial Vegetable Drying Equipment]

Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts.Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, 175-184[Cyclone Dust Collector For Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Capacitive Push Button Membrane Keyboards]

Whitaker,J.R.(1963),One Compartment Commercial Sink Without Drainboard. Analytical Chemistry35(12), 1950-1953[Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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