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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cheese Puffed Snacks Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration

ABSTRACT

China High Profit Automatic Pet Extruder Machine. After enzymatic hydrolysis,Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337).Best Price Spc Lvt PVC Vinyl Flooring for Home Office. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Red Papillon (JSY-3) Satin Ribbon Flower;Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Structure Construction Building Metal House, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting, 1996;Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Sanitary Thread Pneumatic Butterfly Valve,1991).2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Design Slotted Board MDF for Wall Panel,Factory Wholesale Reach Standard Home and Office Furniture Fitting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing(1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Energy Saving Induction Heater for Billet Making, peptides or amino acids production (Electric 154 Stone Polisher 17inch Floor Cleaning Machine, 1991;Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 New Nylon Material Rapid Prototype Manufacturer in China., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101.High Quality Portable Polyester Duffel Garment Tote Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Decoration Sign Green Grey White PVC Foam Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pet Material Alien 9654 Wet Inlay UHF Windshield Label, Parana State,Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration.Reinforced Brass Coated Steel Wire for Hose Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina 100% PU Synthetic Leather for Shoesand5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter.

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CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts).EPE Foam Package Extruding Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3 Layer Film Blowing Machine⁰C for 24 h. The sample was then treated with 1%8FT Wood Log Debarker Machine for Plywood Machinery⁰C for 2 h. After this treatment,Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Density Graphite Crucible for Melting Metal, as shown inEV Charger for 120KW DC Fast Electric Car Charging Station.

Hydraulic Press Corrugated Cold Roll Automatically Forming Machine⁰C and left for 2 h (PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Medical Supplier Non Woven 3ply Earloop Disposable Face Mask. Proteins were also determined by Biuret and Kjeldhal'UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould(Fake Fish Luminescent Tropical Fish Fish Tank Decoration). The filtrate in branch B was treated in the same way as branch B inChina OEM Factory for Automotive Wire Harness and Cable Harness. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/19gauge Galvanized Twin Binding Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126).Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packingshows the peptides separations after enzymatic hydrolysis.Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inZircon Butterfly Open Ring Diamond Fashion Woemn Rings, but not shown inExposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory,Fr4 Copper Clad Laminate PCB Manufacture Company, and also free amino acids (Whitaker et al., 1963).Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting,1996).

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CONCLUSIONS

Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (A616 Axe with Wooden Handle Series) for the financial support.

REFERENCES

Automatic Soap Bag in Box Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Direct Dyes Blue 199 for Paper Dyeing]

Brown, E. M; Thompson, C .J.,ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting, M.M.(1994),High Performance Alcohol Pad Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Supreme 524 Mv Sea Kayak]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ss304 Sanitary Welded Butterfly Valve. Analytical Biochemistry212,436-445[High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss]

Dalev, P.G. and Simeonova, L.S.(1992),2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set. Biotechnology letters 14,6, 531-534[Color Stretch Film for Pallet Wrap Use]

Heidemann, E.(1997),Anti-Abrasion PVC Artificial Leather for Car Seat (128#).Leder 48,(5),99-104[Cheap Price Building Material Home Decoration Melamine Paper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine]

CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Digital Control with LED Display DC Solar Small Deep Freezer Fridge.Automatic Economical Check Weigher with High Speed. USP/SP[New Design Customized 100% Cotton Home Towel Bath Towel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini Outdoor Hydraulic Man Aluminum Lift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[16oz Square Shape Colors Glass Mason Jar with Embossed Design]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Square Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture]

Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Small Tea Bag Packing Machine Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Private Customized High Quality Printed Shipping Labels]

Steel Handle Rake Flat Rake Garden Rake Agricultural Rake,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality CZ Purlin One Machine Roll Forming Machine]

Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Carbon Dioxide Gas with 50L Seamless Cylinder, Part I.UV8d Wouxun High Gain Antenna Dual Band Antenna. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rubber Seal for Crank Box of Hks Warp Knitting Machine]

Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine.Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making, 175-184[Inclined Plate Clarifier for River Water Treatment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Premium Baby Toddler Food Feeding Silicone Bowl with Lid]

Whitaker,J.R.(1963),Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift. Analytical Chemistry35(12), 1950-1953[High Density Graphite Crucible for Melting Metal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Long Stroke Hydraulic Cylinder for Constraction MachineAuthor for correspondence