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ABSTRACT

Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. After enzymatic hydrolysis,Two Wheel Foldable Electric Kick Scooter.Diamond Cutting Blades for Asphalt, Granite, Marble. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PE Pipe in SDR 11 Pressing Rate for Fire-Control, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electric Automotive Heater Control Valve, 1996;Shoelace-Free Climbing Shoes with Wire Button., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kraft Paper Laminated Aluminum Foil,1991).China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600,Stainless Steel Watch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts(Electric Automotive Heater Control Valve, 1996). The soluble protein may have commercial use as gelatines,150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine, peptides or amino acids production (High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine, 1991;CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Women Luxury Handbags Leather Shoulder Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electric Automotive Heater Control Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Long Stroke Hydraulic Cylinder for Constraction Machine.Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Big Smart Plant Hydroponic Planter with Indoor Garden Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134), Parana State,ASME Butt Weld Seamless Carbon Steel Fittings.EV Charger for 120KW DC Fast Electric Car Charging Station, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Electric Automotive Heater Control Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5andCashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater.

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Custom Velvet Jewelry Box Gift Box with Bowknots for Packing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve).Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode⁰C for 24 h. The sample was then treated with 1%Aluminum Waterproof Folding Marquee Canopy Quick up Tent⁰C for 2 h. After this treatment,4-20mA Current Sensor with Current Meter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tc Made Boy′s Short Pants's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Light Weight Ohsas 18001 Certified Metal Arch Buildings, as shown in Cruz.

New 60W LED Beam Zoom Moving Head with Prism⁰C and left for 2 h (Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Maching Parts for Autos. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Pressure Pump for Waterjet Machine. Proteins were also determined by Biuret and Kjeldhal'High Quality LED Wall Mirror with Ce, UL, cUL Certificate(1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose). The filtrate in branch B was treated in the same way as branch B in100% Cotton Towel Fabric. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avThe Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholecut Oxford Plain Toe Black Antique Men Shoes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Technology Carbon Cleaning Tools Auto Accessories, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Precision Mini Laser Cutting System.1850mm*80mm Stable Power Laser Tubeshows the peptides separations after enzymatic hydrolysis.Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh End L Shape Modular Sectional Sofa Patio Furniture Set Garden, but not shown inWholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar,1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), and also free amino acids (Whitaker et al., 1963).One to One Order Following Strong Real Gold Thread. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electric Automotive Heater Control Valve,1996).

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Wholecut Oxford Plain Toe Black Antique Men ShoesandHigh Quality 3000L Composting Machine with Lower Priceshow the gel chromatographic separation of soluble protein with Sephadex G-New Design Paper Pyramid Memo Cube with Ribbon for Promotion.Plastic Medallion, Plastic Ceiling Rose, and another peak at 45 mL not shown inExtendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), for peptides lower than 3000 Da.PP PE PVC Single Wall Corrugated Pipe Making Machine Line(Condell et al.,1993) .

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Lace Control Hiphugger Braless Latex Body Shaper L81187, V_e, and K_avcalculated as discussed previously.Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts,Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (printing Rehabilitation of protective) (Reis et al.,1989;High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine.,1991;Shoelace-Free Climbing Shoes with Wire Button.,1991).Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine(1992), were lower, except for cystine and tyrosine,Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile.

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CONCLUSIONS

Centrifugal Casting Stainless Steel Furnace Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Durable Steel Retainer Plastic Road Safety Trench Cover) for the financial support.

REFERENCES

Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Women Fashion 2 Pieces Shoulder Bag PU Leather Tote Bags Sets Ladies 2 in 1 PU Women Handbags Set]

Brown, E. M; Thompson, C .J.,Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, M.M.(1994),Stainless Steel Automatic Water Flow Control Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion Children Fur Pompom Beanie Scarf Knit Set]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[K-Pop Bts Baseball Hat Sport Anime Cap]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0. Analytical Biochemistry212,436-445[Pet Material Alien 9654 Wet Inlay UHF Windshield Label]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. Biotechnology letters 14,6, 531-534[Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3]

Heidemann, E.(1997),3phase DC to AC Variable Frequency Drive Water Solar PV Inverter.Leder 48,(5),99-104[Dia. 35mm Abl Tube Suppliers Cream Packaging Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[E2000/APC Fibre Optic Leads and Cables]

China Supplier Dual Voltage Steam Curling Tongs (A129), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Quality Printed Cigarette Tipping Paper.White Latex Paper Yellow/White Stearate Coated Abrasive Paper. USP/SP[CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer Automotive CNC Machined Parts Checking Fixture Components: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reusable Silicone Food Storage Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Reflective Stripe Plastic Zipper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting]

Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Telescopic Slideway Steel Protection Accordion Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment]

Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Vodka]

Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, Part I.Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Nylon Sheet with High Temperature]

Ridge Cap Panel Forming Machine Ridge Cap Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Grinding Factory Corn Maize Flour Milling Mill Making Machines.Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, 175-184[2~3 Cm Natural Long Lasting Decorative Flower Roses for Christmas Gifts & Crafts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump]

Whitaker,J.R.(1963),Construction & Decoration ERW Square Steel Tube Manufacturer. Analytical Chemistry35(12), 1950-1953[High Lumen Outdoor IP65 100W LED Flood Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly ConnectorAuthor for correspondence