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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Manufacturer Automotive CNC Machined Parts Checking Fixture Components. After enzymatic hydrolysis,Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting.Telescopic Slideway Steel Protection Accordion Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ridge Cap Panel Forming Machine Ridge Cap Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Grinding Factory Corn Maize Flour Milling Mill Making Machines, 1996;Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Construction & Decoration ERW Square Steel Tube Manufacturer,1991).Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet . Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Household Use Food Packing 8011 Aluminum Foil,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale(Grinding Factory Corn Maize Flour Milling Mill Making Machines, 1996). The soluble protein may have commercial use as gelatines,Plastic Case Navigation System Tooling Injection Mold, peptides or amino acids production (Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set, 1991;8X8mm Silver Expanded Metal Mesh in Hot Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100% Durable Flooring Roll for Hotel Office School and Health Project Floor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Grinding Factory Corn Maize Flour Milling Mill Making Machines, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pet Bottle Washing Recycling Line in China.Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)).Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price⁰C for 24 h. The sample was then treated with 1%Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006⁰C for 2 h. After this treatment,Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Food Grade PE Coated Kraft Paper for Paper Cup, as shown in3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection.

Stainless Steel Single Filter Cartridge Housing for Food Grade⁰C and left for 2 h (750ml Long Neck Dark Green Color Glass Bottle for Wine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Stainless Steel Investment Casting Bearing for Car Parts. Proteins were also determined by Biuret and Kjeldhal'Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System(15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades). The filtrate in branch B was treated in the same way as branch B inConventional Fire Alarm Control Panel Fire Alarm System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%30t/H RO Reverse Osmosis System Water Treatment Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Price of Custom Brands Granite Sink for Home(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glass Board Automatic Sloping Printing Table⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Two Beams Active Infrared Intrusion Detector, Photo Beam, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Motorized Rail Car Heavy Duty Trailer Vehicle.Full Cream Milk Powder Filling Machine (XFF-G)shows the peptides separations after enzymatic hydrolysis.South Africa AC Power Cord Plug with C5 Connector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), but not shown inUnion NPT Bsp BSPT Stainless Steel Pipe Fittings,PCB Design, PCB Assembly for Electric Home Appliance, and also free amino acids (Whitaker et al., 1963).Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Grinding Factory Corn Maize Flour Milling Mill Making Machines,1996).

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CONCLUSIONS

High Dust Holding Capacity HEPA Filter with Separator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sc/APC Fiber Optic Fast Hot Melt Connector) for the financial support.

REFERENCES

Crystal Pendant Lamp, DIY Shade (WHP-455), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Industrial High Speed Doors]

Brown, E. M; Thompson, C .J.,1.5tons Mini Site Dumper SD15, M.M.(1994),1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cast Iron Frame-Plate Chemical Filter Press]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Self Recovery PVC High Speed Roller Shutter. Analytical Biochemistry212,436-445[Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Ductile Gully Iron Guard Drainage Manhole Cover. Biotechnology letters 14,6, 531-534[Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator]

Heidemann, E.(1997),High Precision Rail Slider 20mm Diameter PMI Linear Guideway.Leder 48,(5),99-104[Mild Steel U Channel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Supplier Laboratory Mixer for Comstic]

3phase DC to AC Variable Frequency Drive Water Solar PV Inverter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Price Cat Scratching Trees Cat Tree Luxury.China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump. USP/SP[Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Adult Neoprene Sport Summer Beach Aqua Swimming Shoes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Espresso Coffee Maker Machine with Removeable Filter Basket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Strut Channel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[NPK compound fertilizer granulating machine]

Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Profile Sp-Level Linear Rail for 3D Printer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car]

Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[7628 Fiberglass Cloth/Fabric]

Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, Part I.High Quality Paulownia Wood Board Wood Drying Kilns for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Single PC Sachet Bag Wet Tissue Machine]

Seamless Boxer Brief for Men, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning.Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, 175-184[Cat 7 Cable Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[NBR EPDM Neoprene Silicone Rubber Sheet for Seal]

Whitaker,J.R.(1963),Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies. Analytical Chemistry35(12), 1950-1953[Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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