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ABSTRACT

Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event. After enzymatic hydrolysis,Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress.Sc/APC Fiber Optic Fast Hot Melt Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium, 1996;Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price,1991).Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags,4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Grade PE Coated Kraft Paper for Paper Cup(Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Single Filter Cartridge Housing for Food Grade, peptides or amino acids production (Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, 1991;OEM Stainless Steel Investment Casting Bearing for Car Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-30t/H RO Reverse Osmosis System Water Treatment Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Price of Custom Brands Granite Sink for Home.A36 Galvanized Building Steel Structure Column H Steel Column-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glass Board Automatic Sloping Printing Table(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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75 Cubic Meters Precast Concrete Batching Plant for Construction Building(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Design Steel Prefab Layer Cage Chicken House Poultry Farm).Motorized Rail Car Heavy Duty Trailer Vehicle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%South Africa AC Power Cord Plug with C5 Connector⁰C for 24 h. The sample was then treated with 1%PCB Design, PCB Assembly for Electric Home Appliance⁰C for 2 h. After this treatment,Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)NPK compound fertilizer granulating machine, reduce dust pollution's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Shrink Sleeve Bottle Label for Can Packing, as shown in110V-240V LED Filament Bulb Globe Cafe Patio String Light.

OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable⁰C and left for 2 h (Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2. Proteins were also determined by Biuret and Kjeldhal'300mm Stroke Linear Module Sliding Bearing for CNC System(High Precision iPhone Cases Fiber Laser Marking Machine). The filtrate in branch B was treated in the same way as branch B inConstruction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Luxury Hotel Furniture Antique Design Marble Side Table. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Dust Holding Capacity HEPA Filter with Separator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1.5tons Mini Site Dumper SD15⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Self Recovery PVC High Speed Roller Shutter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater) for the financial support.

REFERENCES

Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1650mm*80mm Non-Metalic Materials Laser Tube]

Brown, E. M; Thompson, C .J.,Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve, M.M.(1994),17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS.The Journal of the American Leather Chemists Association (JALCA)89(7)[Floral Kids PVC School Raincoat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reusable CAT6 UTP RJ45 8P8C Modular Plug. Analytical Biochemistry212,436-445[Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator]

Dalev, P.G. and Simeonova, L.S.(1992),3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights. Biotechnology letters 14,6, 531-534[New Model Water Source Heat Pump (SLW100D)]

Heidemann, E.(1997),Good Quality OEM Stainless Steel Casting Rings.Leder 48,(5),99-104[Modern Top Grade Square Glass Dining Room Tables and Chairs Stainless Steel Tables Used]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags]

Pet Bottle Washing Recycling Line in China, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet.Customizable Logo Plastic Safety Walking Reflectors Chinese Factory. USP/SP[Manual Glass Lifting Machine for Making Double Glass]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rotating Sprinkler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

Flame Retardant FRP/GRP Round Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Psa Oxygen Generator for Industry (ISO9001, CE)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Community IC Access Card Induction ID Time Card Smart Card]

Flame Retardant FRP/GRP Round Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Waste Paper Stripper]

Flame Retardant FRP/GRP Round Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, Part I.P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag]

Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom small plastic printed box for keychain (PET box).Fence Mesh Welding Machine, 175-184[Chrome Paint System High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Extruder Floating/Sinking Fish Feed Pellet Dryer]

Whitaker,J.R.(1963),20 Years Factory! Double Channel Surgical Syringe Pump. Analytical Chemistry35(12), 1950-1953[HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder MachineAuthor for correspondence