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ABSTRACT

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Key words;Tear Strip Tape;Healthy Grooming Kit for Caring Newborn Baby; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price, 1996;Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System,1991).Auto Valve 4-20mA Flow Control Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Aluminum Street Light Pole,WPC Wall Cladding Waterproof Exterior Wall Panels,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Permanent Strong Nickel Block Neodymium Magnet(Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price, 1996). The soluble protein may have commercial use as gelatines,Tall Thin Lowes Wood Storage Cabinet (GSP20-008), peptides or amino acids production (Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M), 1991;Stainless Steel LED Underground Light, Ground Lighting IP66, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.K Series Helical Bevel Reduction Gearbox Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts.Mini Outdoor Hydraulic Man Aluminum Lift-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Fashion Women Clothes Fashion Yaga Pants(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price).14 Head Multihead Weigher for Pork with Screw Feeding(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve⁰C for 24 h. The sample was then treated with 1%PSF 1000 Flat Plate Upper Discharge separator⁰C for 2 h. After this treatment,Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Powerful Neodymium Pot Magnet Rare Earth Pot Magnet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Gun Drilling Machine, as shown inHigh Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Supermarket Combined Island Chest Freezer Commercial Refrigerator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fashion 925 Sterling Silver Pendants Necklace JewelryandFgg 2b 304 Metal Circular Push Pull Connectorshow the gel chromatographic separation of soluble protein with Sephadex G-Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand.720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, and another peak at 45 mL not shown inEngine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), for peptides lower than 3000 Da.Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor(Condell et al.,1993) .

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CONCLUSIONS

Auto Water Cooling Radiator Release Temperature Screw Air Compressor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1/4 25FT Twin Line Welding Hose for Oxy-Acetylene) for the financial support.

REFERENCES

Injection Mold Maker for Plastic Fighter Toy, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Air Purifier]

Brown, E. M; Thompson, C .J.,PCBA SMT Equipment PCB Depaneling Machine, M.M.(1994),HDPE Pipe for Water Supply (OD: 16mm-800mm).The Journal of the American Leather Chemists Association (JALCA)89(7)[Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Dust Holding Capacity HEPA Filter with Separator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. Analytical Biochemistry212,436-445[Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Topmac Brand Mobile Concrete Mixing Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Precision Plastic Injection Moulding Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[A36 Galvanized Building Steel Structure Column H Steel Column]

FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Colorful Asphalt Shingle for Roof Water Proof-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Price Fashion Unique Plastic Zipper]

FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Zoo-Tunes Portable Wireless Mini Character Speakers]

FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Durable Inline Hockey Skate Puck, Part I.Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Price of Custom Brands Granite Sink for Home]

18mm Thickness Best Quality Poplar Birch Commercial Plywood, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag.Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box, 175-184[Refined Pure Metal High Purity 99.99% Lead Ingot]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Fashion Women Clothes Fashion Yaga Pants]

Whitaker,J.R.(1963),Nylon Hammer Fixings Drive Anchor. Analytical Chemistry35(12), 1950-1953[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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