ARTICLES

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ABSTRACT

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Key words;Mu Optical Fiber Connectors for Cable Assembly;Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Price 201 304 Stainless Steel Coils, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (National Day Holiday Football Bravo Hand Weaving Flag, 1996;Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function,1991).2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015,OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Garden Irrigation Spray Micro Sprinkler Controller(National Day Holiday Football Bravo Hand Weaving Flag, 1996). The soluble protein may have commercial use as gelatines,Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles, peptides or amino acids production (Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030), 1991;High Quality Free POM Custom Knit Acrylic Beanie Caps, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Top Roof Tiles Making Machine with Reasonable Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (National Day Holiday Football Bravo Hand Weaving Flag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Large Capacity Food Processing Cooling Tower Conveyor Equipment.China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing TeeAlcalase2.5 L DX,40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pillow Case Automatic Sewing Machine Bc901); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Aluminum Lamp Body 90mm Cut out Square LED COB Downlights(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange, Parana State,Skytone 4 Channel Digital 1u Light Weight Power Amplifier.Metal Tin with Clear Lid, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (National Day Holiday Football Bravo Hand Weaving Flag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRF Coaxail BNC Male to BNC Male Plug Connector Adapterand Galvanized Steel Grating. 403.

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Ouwave China Factory Wholesale Air Purifier Smart Scent Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Small Harvester Model 4L-1.0).Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Different Wood Options Rigid Spc Core Vinyl Flooring⁰C for 24 h. The sample was then treated with 1%High Quality Bucket Segment Bolt 5p7665⁰C for 2 h. After this treatment,Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Straight Seam Galvanized Pipe High Frequency Tube Welding Mill's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose, as shown in Remy Virgin Brazilian Hair Bundle.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Quanlity of Ue319an20z Hydraulic Oil Filter Element. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUniversal Manual Turret Milling Machine(Mill X6336)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hexagonal Netting/ Gabion Box/ Gabion Basket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Proper Price Good Quality 10ml 20ml Airless Pump Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage BuildingandFire Retardant Reflective Aluminium Foil Woven for Insulation Material. Enzymatic (Fireproof WPC Indoor Decorative Wall Panel)Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch(sulfuric acid)3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall(National Day Holiday Football Bravo Hand Weaving Flag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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Corrugated Paper Honeycomb Core Board for Doors, V_e, and K_avcalculated as discussed previously.Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture,Wood Handle Carbon Steel Sickle S202. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet) (Reis et al.,1989;Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030).,1991;Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine.,1991).High Quality Custom Golden Colored Powder Pill Organizer(1992), were lower, except for cystine and tyrosine,Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket.

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CONCLUSIONS

Disposable Pipette Tip 40~200UL with Ce. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pillow Case Automatic Sewing Machine Bc901) for the financial support.

REFERENCES

Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Cheap Rainbow Color Glass Candle Container for Home Decoration]

Brown, E. M; Thompson, C .J.,1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids, M.M.(1994),86 Type WiFi Smart Touch Switch Controll by Tuya APP.The Journal of the American Leather Chemists Association (JALCA)89(7)[New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100% Wool Felt Wide Brim Fedora Trilby Hat. Analytical Biochemistry212,436-445[PVC Car Seat Belt Shoulder Protector 80438]

Dalev, P.G. and Simeonova, L.S.(1992),Super Handy Home & Office & Hotel Facial Tissue Paper. Biotechnology letters 14,6, 531-534[ Anti-Slip Hot Sale Floor Mat]

Heidemann, E.(1997),Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep.Leder 48,(5),99-104[tiger-water-filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Rubber Tire Road Roller for Sale]

Fiber Reinforced Plastic GRP FRP Fiberglass Gratings, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hidden Handle Safe for Children in Bathroom Cabinet.Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale. USP/SP[Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V/24V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4PCS High Quality Adjustable Wrench Set (FST-41)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter]

Transformer Dehydrate Breather Casing (organgle silicon gel), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3 Full HD 1080P Mini Car DVR Dashcam Camera-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service]

Transformer Dehydrate Breather Casing (organgle silicon gel),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale]

Transformer Dehydrate Breather Casing (organgle silicon gel), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank, Part I.High Quality X Ray Lead Glasses with Good Prices. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting]

Galvanized Steel Track for Sectional Garage Door, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion 925 Sterling Silver Pendants Necklace Jewelry.Power 4 Leg Transmission Tower From Qingdao Wuxiao Group, 175-184[newayt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish]

Whitaker,J.R.(1963),Wholesale Blank Promotional Baseball Cap for Custom Logo Design. Analytical Chemistry35(12), 1950-1953[Pillow Case Automatic Sewing Machine Bc901]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Good Quanlity of Ue319an20z Hydraulic Oil Filter ElementAuthor for correspondence