ARTICLES

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ABSTRACT

Professional Sound System Vt215 Line Array Speaker. After enzymatic hydrolysis,Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC.LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Duct Evaporative Air Cooler for Warehouse Factory;Micro Sprinkler for Water Saving Irrigation; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden, 1996;ABS Shell Plastic Injection Molding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press,1991).450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manual Glass Lifting Machine for Making Double Glass,Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Towed Bitumen Mixer Slj5(High End L Shape Modular Sectional Sofa Patio Furniture Set Garden, 1996). The soluble protein may have commercial use as gelatines,High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price, peptides or amino acids production (3D Inkjet Printing Waterproof Interior Kitchen Wall Tile, 1991;IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Viscose Sodium Sulphate Anhydrous by Sateri. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Orange Silica Gel 2-4 mm with High Quality.2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:aluminium discs for fry pan alloy 1060 1100 1050 3003(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fgg 2b 304 Metal Circular Push Pull Connector).Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100ml Plastic E Liquid Vape Cig Juice Dropper Bottle⁰C for 24 h. The sample was then treated with 1%Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building⁰C for 2 h. After this treatment,Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2-25mm Co-Extruded Highlight PVC Foam Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Engraving Machine Laser Marking for Assembly Line, as shown inEngine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Power Solar Inverter with Full Power 3000W Converter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Zoo-Tunes Portable Wireless Mini Character Speakers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CF8m Air Actuated Valve Dn15, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Professional Silicone Rubber Gasket for Doors Windows.OEM Custom Forged Auto Partsshows the peptides separations after enzymatic hydrolysis.Top Quality 99.95% Molybdenum Square Plates(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDnv 271 En 12079 3.5 Cbm Offshore Mud Skip, but not shown inHigh Dust Holding Capacity HEPA Filter with Separator,HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, and also free amino acids (Whitaker et al., 1963).Flat Face Stainless Steel Female Jic Quick Release Coupling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden,1996).

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CONCLUSIONS

PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Fashion Ladies Backpack PU Leather Backpack) for the financial support.

REFERENCES

65 Polyester 35 Cotton Fabric Camouflage Printed Fabric, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Large Hydraulic Cylinders]

Brown, E. M; Thompson, C .J.,Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, M.M.(1994),Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Zoo-Tunes Portable Wireless Mini Character Speakers]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price of Custom Brands Granite Sink for Home]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers. Analytical Biochemistry212,436-445[Wholesale Fashion Women Clothes Fashion Yaga Pants]

Dalev, P.G. and Simeonova, L.S.(1992),Rolling Shutter Door Hardwares, Link Pipe, Link Clip. Biotechnology letters 14,6, 531-534[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

Heidemann, E.(1997),200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine.Leder 48,(5),99-104[Air Filter 28113-3x000]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee]

Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve.FUJI Small Home Lift for Sale. USP/SP[Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heavy Duty 16000L High Pressure Sewer Cleaning Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sinotruk 12 Wheels 35]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Office Sofa Furniture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[E0303 300n Activator Chiropractic Impulse Adjusting Instrument]

Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ce Jacket Heated Water Chocolate Holding Tank Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Skytone 4 Channel Digital 1u Light Weight Power Amplifier]

Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack]

Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lanyards Ribbons Screen Printing Machine, Part I.China Factory New-Product-Best-Quality-Customized-Metal-Pressing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Recycle Finger Joint Core Film Faced Shuttering Plywood.Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5, 175-184[Chemical Fertilizer Dry roller compactor granulation machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)]

Whitaker,J.R.(1963),Beer Made in China OEM Abv 8.0%Vol Lager Beer. Analytical Chemistry35(12), 1950-1953[3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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