ARTICLES

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ABSTRACT

Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags. After enzymatic hydrolysis,2-25mm Co-Extruded Highlight PVC Foam Board.CNC Engraving Machine Laser Marking for Assembly Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1);Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, 1996;nellcor non-oximax 7pin infant disposable sensor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Power Solar Inverter with Full Power 3000W Converter,1991).Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts,Zoo-Tunes Portable Wireless Mini Character Speakers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CF8m Air Actuated Valve Dn15(12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, 1996). The soluble protein may have commercial use as gelatines,Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, peptides or amino acids production (Premium Baby Toddler Food Feeding Silicone Bowl with Lid, 1991;Hardcover Bear′s Story Children Book Custom Printing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Liquid Hardener Setting Cement Membrane Curing Concrete. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Silicone Rubber Gasket for Doors Windows.Top Quality 99.95% Molybdenum Square Plates-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Flat Face Stainless Steel Female Jic Quick Release CouplingAlcalase2.5 L DX,Aluminium Glass Dropper for Glass Dropper Bottle, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-L133pbd Common Rail Delphi Nozzle for Ford Mondeo); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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One Compartment Commercial Sink Without Drainboard(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine).Ge Holter Cable Snap Type Holter ECG Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5⁰C for 24 h. The sample was then treated with 1%PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass⁰C for 2 h. After this treatment,65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump, as shown inMen Genuine Leather Sling Shoulder Satchel Messenger Bag.

OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers⁰C and left for 2 h (Community IC Access Card Induction ID Time Card Smart Card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rolling Shutter Door Hardwares, Link Pipe, Link Clip. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine. Proteins were also determined by Biuret and Kjeldhal'China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve(Automatic Waste Paper Stripper). The filtrate in branch B was treated in the same way as branch B inNew Weekend Football Necessary Sports Travel Backpack Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,FUJI Small Home Lift for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWater Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lanyards Ribbons Screen Printing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Factory New-Product-Best-Quality-Customized-Metal-Pressing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic BagandRecycle Finger Joint Core Film Faced Shuttering Plywood. Enzymatic (Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5)Beer Made in China OEM Abv 8.0%Vol Lager Beer(sulfuric acid)Cat Cardboard House Pet Product Accessories(12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%EPDM Crossfit Gym Mat/Rubber Interlocking Flooring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%EPDM Crossfit Gym Mat/Rubber Interlocking Flooring, flow rate of 0.1 mL/min., and l = 620 nm.

Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor.Extruder Floating/Sinking Fish Feed Pellet Dryershows the peptides separations after enzymatic hydrolysis.Hf Vacuum Dryer Machine for Drying Wood(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line, but not shown inNew Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine,Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table, and also free amino acids (Whitaker et al., 1963).52cc Gasoline Paddy Cutter Two Handle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck,1996).

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Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould, V_e, and K_avcalculated as discussed previously.Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives,China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Family Nonstick Cast Iron Frying Pan) (Reis et al.,1989;Premium Baby Toddler Food Feeding Silicone Bowl with Lid.,1991;nellcor non-oximax 7pin infant disposable sensor.,1991).400-600 Grit 201 304 Grade Polished Stainless Steel Pipe(1992), were lower, except for cystine and tyrosine,Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply.

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CONCLUSIONS

Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (L133pbd Common Rail Delphi Nozzle for Ford Mondeo) for the financial support.

REFERENCES

5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Hardware Cast Iron Casting Auto Parts]

Brown, E. M; Thompson, C .J.,Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter, M.M.(1994),Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model.The Journal of the American Leather Chemists Association (JALCA)89(7)[Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Die-Casting Aluminium Radiator (RN808-500)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Car Interior Accessories Customized Car Seat Cover. Analytical Biochemistry212,436-445[Tc Made Boy′s Short Pants]

Dalev, P.G. and Simeonova, L.S.(1992),Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design. Biotechnology letters 14,6, 531-534[Custom 2 Ring Binder PP Lever Arch Clip File Folder]

Heidemann, E.(1997),10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition.Leder 48,(5),99-104[Modern Metal Base Brown Color Coffee Shop Leisure Sofa]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board]

Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet.Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad. USP/SP[QJ-M Moving small manual brick making machine with low cost]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM/ODM Raw Material MDF HDF Laminate Door: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.acrubberfactory.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Metal Stamping Parts with Anodizing]

7628 Fiberglass Cloth/Fabric, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainles Steel Cartridge Filter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel]

7628 Fiberglass Cloth/Fabric,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Australia Standard Hot Sale 750ml Glass Wine Bottle]

7628 Fiberglass Cloth/Fabric, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:750ml Long Neck Dark Green Color Glass Bottle for Wine, Part I.Floral Kids PVC School Raincoat. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Cat Scratching Trees Cat Tree Luxury]

MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock.Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, 175-184[Dsola Wholesaler Custom Waterproof T3 Solar Power Connectors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle]

Whitaker,J.R.(1963),78mm Aluminum Gold Color Plastic Jar Screw Cap. Analytical Chemistry35(12), 1950-1953[Large Span Steel Structrure for Warehouse From China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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