ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-New Design Customized 100% Cotton Home Towel Bath Towel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Toughened Railing Glass

ABSTRACT

Delrin Sheets with Good Wear Resistance. After enzymatic hydrolysis,High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery.Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies;Plastic Gas and Water End Protective Covers (YZF-H26); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.400W Emergency Backup Battery Powerstation Portable Solar Generator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof, 1996;Automatic Electric Hydraulic Metal Bending Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (6082 and 6061 Aluminum CNC Machining Parts for Aircraft,1991).1054 FRP Pressure Vessel for Water Softener & Water Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System,Non Woven Fabric for Shoe Lining Shoe Inner Good Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking(Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof, 1996). The soluble protein may have commercial use as gelatines,HDPE Large Diameter Hollowness Wall Winding Pipe Production Line, peptides or amino acids production (Non-Woven Medical Outside Earloop Mask Making Machine, 1991;Professional Family Nonstick Cast Iron Frying Pan, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashionable Clothing Garment Paper Swing Tags (ST-01).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Horizontal Directional Drilling Machine SHD28. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electrial Galvanized Conduit Box/Gi Pipe Fitting.Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High quality Brass Forged Gate Valve with Casting Iron Handle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Mini LED Flood Light SMD Flood Light 150W, Parana State,Toughened Railing Glass.Low Price Fashion Unique Plastic Zipper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale Energy Saving Induction Heater for Billet MakingandGrinding Factory Corn Maize Flour Milling Mill Making Machines.

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Silica Gel- Indicating Blue to Pink After Adsorption(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade).Offer High Quality Square and Round Cast Iron Manhole Cover(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%White Tight Long Sleeves off Shoulders Celebration Bandage Dress⁰C for 24 h. The sample was then treated with 1%OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors⁰C for 2 h. After this treatment,4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Road Safety Reflective Waterproof Plastic Reflectors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary, as shown inPlastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHaivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multifunction Copper Busbar Processing for Aluminum Material⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Genuine Leather Sling Shoulder Satchel Messenger Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric DC 24V Geared Motor for Auto Doors, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Integrated Hydraulic Steering Control Units (BZZ1F SCU), V_e, and K_avcalculated as discussed previously.New Model Water Source Heat Pump (SLW100D),Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp) (Reis et al.,1989;Non-Woven Medical Outside Earloop Mask Making Machine.,1991;Automatic Electric Hydraulic Metal Bending Machine.,1991).Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube(1992), were lower, except for cystine and tyrosine,10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets.

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CONCLUSIONS

Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cog Mono Round Display LCD Module) for the financial support.

REFERENCES

High Capacity Vibrating Screen Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality L Type Anchor Bolt]

Brown, E. M; Thompson, C .J.,Electrochemical Polishing Tungsten Evaporation Materials, M.M.(1994),Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Light Weight Ohsas 18001 Certified Metal Arch Buildings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Performance Alcohol Pad Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White UTP Cat5e UK Type Tooless RJ45 Keystone Jack. Analytical Biochemistry212,436-445[High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill. Biotechnology letters 14,6, 531-534[Reusable CAT6 UTP RJ45 8P8C Modular Plug]

Heidemann, E.(1997),CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts.Leder 48,(5),99-104[660 ) - Lab Mixing Mill]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top Quality Sanitary Tube Shell Tube Heat Exchanger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China High Profit Automatic Pet Extruder Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Extruded Polystyrene Foam Board Custom Edges S/L]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dermal Filler Injectable Hyaluronic Acid for Nose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ High Pressure Lay Flat Hose for Industrial Discharge]

Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Price Spc Lvt PVC Vinyl Flooring for Home Office-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine]

Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[HDPE Pipe for Water Supply (OD: 16mm-800mm)]

Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Red Papillon (JSY-3) Satin Ribbon Flower, Part I.Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch]

Steel Structure Construction Building Metal House, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting.Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, 175-184[qilangmotor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Diameter Coil Compression Spring From Factory]

Whitaker,J.R.(1963),Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. Analytical Chemistry35(12), 1950-1953[Stainless Steel French Earring Hook for Jewelry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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