ARTICLES

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ABSTRACT

Men Genuine Leather Sling Shoulder Satchel Messenger Bag. After enzymatic hydrolysis,Electric DC 24V Geared Motor for Auto Doors.Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reusable Silicone Food Storage Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dental Emax Empress Crown From China Dental Lab, 1996;Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer,1991).Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dubai Products Rectified Tile for Wall,ANSI B16.5 Stainless Steel SS316 Forging Blind Flange,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection(Dental Emax Empress Crown From China Dental Lab, 1996). The soluble protein may have commercial use as gelatines,2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, peptides or amino acids production (Integrated Hydraulic Steering Control Units (BZZ1F SCU), 1991;New Model Water Source Heat Pump (SLW100D), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dental Emax Empress Crown From China Dental Lab, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325.High Capacity Vibrating Screen Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electrochemical Polishing Tungsten Evaporation Materials(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China High Profit Automatic Pet Extruder Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electrial Galvanized Conduit Box/Gi Pipe Fitting).Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price Spc Lvt PVC Vinyl Flooring for Home Office⁰C for 24 h. The sample was then treated with 1%Red Papillon (JSY-3) Satin Ribbon Flower⁰C for 2 h. After this treatment,Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel Structure Construction Building Metal House's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting, as shown inModern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Energy Saving Induction Heater for Billet Making(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 New Nylon Material Rapid Prototype Manufacturer in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill) for the financial support.

REFERENCES

Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Inflatable Cheering Stick for Promotional Gift]

Brown, E. M; Thompson, C .J.,Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, M.M.(1994),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould.The Journal of the American Leather Chemists Association (JALCA)89(7)[Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat. Analytical Biochemistry212,436-445[UV Resistant Single Component Water Based Polyurethane Waterproof Coating]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[TV Stand Carving European LED Lights Heating Fireplace (320SS)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[150 Ton Hydraulic Press Machine for Cooking Pot Making]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[E27 Reading Desk Lamp Vintage]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SBR Round DOT Horse Stall Mat]

19gauge Galvanized Twin Binding Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile]

19gauge Galvanized Twin Binding Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fr4 Copper Clad Laminate PCB Manufacture Company]

19gauge Galvanized Twin Binding Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6.35mm Audio Connector Plug Jack, Part I.Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape]

Whitaker,J.R.(1963),Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor. Analytical Chemistry35(12), 1950-1953[Stainless Steel LED Underground Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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