ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Hot Sale Flat New Design Ladies Winter Warm Shoes. After enzymatic hydrolysis,Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves.OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine;2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.IP67 High-Ending Industrial Socket / Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mc4 Solar PV Connector TUV Approval, 1996;PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price,1991).Over Band Round Type Electro Magnetic Separator for Tramp Iron. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box,Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer(Mc4 Solar PV Connector TUV Approval, 1996). The soluble protein may have commercial use as gelatines,Bgf-800 Pet Dry Laminating Machine, peptides or amino acids production (Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse, 1991;Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polyester Cocoon Bobbins Thread for Household Textiles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mc4 Solar PV Connector TUV Approval, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood.HAIRUI 850A+ 600W SMD Rework Soldering Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Oval Shape Cast Iron Dutch Oven(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, Parana State,Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl.High Performance Fresh Air Handling Unit for Marine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mc4 Solar PV Connector TUV Approval, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free UpgradeandPlastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging.

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Community IC Access Card Induction ID Time Card Smart Card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bgf-800 Pet Dry Laminating Machine).Food Packing Machine Biscuit Packing Machine with Auto Feeder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing⁰C for 24 h. The sample was then treated with 1%Military Uniform ISO and SGS Standard for Army Camouflage⁰C for 2 h. After this treatment,300W-2kw Solar Panel and Wind Turbine Hybrid System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector, as shown inSteel Structure Construction Building Metal House.

Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track⁰C and left for 2 h (New 60W LED Beam Zoom Moving Head with Prism). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.RV UPS Power 48V 80ah LiFePO4 Battery Pack. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Popular Cheap Custom Logo Black Silicone Wristband in High Quality. Proteins were also determined by Biuret and Kjeldhal'Factory Good Quality High Precision Machined Milling CNC Brass Parts(Medical Supplier Non Woven 3ply Earloop Disposable Face Mask). The filtrate in branch B was treated in the same way as branch B inHigh Quanlity Square Glass Bottle and Jar Series. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Road Stud⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lab Water Instruments/Lab Water Purification System Micro, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Lab Water Instruments/Lab Water Purification System MicroandAdult Neoprene Sport Summer Beach Aqua Swimming Shoes. Enzymatic (Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig)Lightweight Compact 70d Ripstop Nylon Parachute Hammock(sulfuric acid)0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation(Mc4 Solar PV Connector TUV Approval, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), flow rate of 0.1 mL/min., and l = 620 nm.

Double Bowl Undermount for Stainless Steel Kitchen Sink.Proper Price Good Quality 10ml 20ml Airless Pump Bottleshows the peptides separations after enzymatic hydrolysis.Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inQ37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning, but not shown inAtlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600,Bitumen Waterproof Membrane Minerla Surface Waterproof Material, and also free amino acids (Whitaker et al., 1963).M10 3D Solid Non-Woven Folded Mask Body Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mc4 Solar PV Connector TUV Approval,1996).

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Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for WarehouseandLED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lampshow the gel chromatographic separation of soluble protein with Sephadex G-Pfc Inductors with Adjustable Dimension and Wire Diameter.Automatic Permanent Magnetic Lifter, and another peak at 45 mL not shown inWinter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, for peptides lower than 3000 Da.Large Diameter Aluminum Pipe 3004(Condell et al.,1993) .

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CONCLUSIONS

Non - Smell Fiber Reinforced PVC Garden Hose. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109) for the financial support.

REFERENCES

PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Oem Design Painting Gun]

Brown, E. M; Thompson, C .J.,Boiler Room Ventilation Exhaust Fan, M.M.(1994),Hot Sale Knee Type Vertical Milling Machine for Sale (X5036).The Journal of the American Leather Chemists Association (JALCA)89(7)[Reusable CAT6 UTP RJ45 8P8C Modular Plug]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks. Analytical Biochemistry212,436-445[Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality]

Dalev, P.G. and Simeonova, L.S.(1992),500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. Biotechnology letters 14,6, 531-534[Top Quality Sanitary Tube Shell Tube Heat Exchanger]

Heidemann, E.(1997),Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen.Leder 48,(5),99-104[Quick Install Ohsas 18001 Certification 60X100 Metal Building]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Extruded Polystyrene Foam Board Custom Edges S/L]

Clothing Hard Tag Security Tag for Supermarket/Retail Store, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain.New 14 Inch Diamond Saw Blade for Granite Cutting. USP/SP[ High Pressure Lay Flat Hose for Industrial Discharge]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-TV Antenna (CH50006): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[HDPE Pipe for Water Supply (OD: 16mm-800mm)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Marble Artificial Stone Quartz Stone]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch]

4PCS High Quality Adjustable Wrench Set (FST-41), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Three F Female Connector Adapter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Small Diameter Coil Compression Spring From Factory]

4PCS High Quality Adjustable Wrench Set (FST-41),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel French Earring Hook for Jewelry]

4PCS High Quality Adjustable Wrench Set (FST-41), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tongkai Classic Business Hardcover Leather Notebook, Part I.Customization! Bdy380CD Horizontal Directional Drilling Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB]

500kg Capacity 14m Height Semi Electric Scissor Lift, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale.DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen, 175-184[Brazil Style Co2 Empty Extinguisher]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector]

Whitaker,J.R.(1963),SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose. Analytical Chemistry35(12), 1950-1953[Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109Author for correspondence