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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Diamond Cutting Blades for Asphalt, Granite, Marble. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Best Selling Road Reflective Rectangle Guardrail Delineator Reflector

ABSTRACT

3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na. After enzymatic hydrolysis,PE Pipe in SDR 11 Pressing Rate for Fire-Control.Electric Automotive Heater Control Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Shoelace-Free Climbing Shoes with Wire Button;Kraft Paper Laminated Aluminum Foil; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, 1996;Stainless Steel Watch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts,1991).150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine,CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Women Luxury Handbags Leather Shoulder Bag(Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, 1996). The soluble protein may have commercial use as gelatines,P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, peptides or amino acids production (Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel, 1991;Long Stroke Hydraulic Cylinder for Constraction Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Big Smart Plant Hydroponic Planter with Indoor Garden Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Anti-Corrosion Fiberglass Sheet and Roll.Durable Steel Retainer Plastic Road Safety Trench Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Low Temperature Water Cooled Scroll Water Chiller(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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4-20mA Current Sensor with Current Meter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c).Tc Made Boy′s Short Pants(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Light Weight Ohsas 18001 Certified Metal Arch Buildings⁰C for 24 h. The sample was then treated with 1%New 60W LED Beam Zoom Moving Head with Prism⁰C for 2 h. After this treatment,CNC Maching Parts for Autos. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Pressure Pump for Waterjet Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality LED Wall Mirror with Ce, UL, cUL Certificate, as shown inCNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery.

Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack⁰C and left for 2 h (Industrial Bedsheet Folding Machine (ZD-3000)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel. Proteins were also determined by Biuret and Kjeldhal'Die-Casting Aluminium Radiator (RN808-500)(1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System). The filtrate in branch B was treated in the same way as branch B inOil Pressure Sensor Switch for Detroit Diesel Dd15 Dd13 50 Mercedes-Benz Atego Axor Actros Adblue 600 609 4000 A0071530828. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholecut Oxford Plain Toe Black Antique Men Shoes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Technology Carbon Cleaning Tools Auto Accessories(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom 2 Ring Binder PP Lever Arch Clip File Folder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10mlandCNC Laser Customized Telescopic Steel Cover. Enzymatic (High Precision Mini Laser Cutting System)Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong(sulfuric acid)1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195)(Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%One to One Order Following Strong Real Gold Thread, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%One to One Order Following Strong Real Gold Thread, flow rate of 0.1 mL/min., and l = 620 nm.

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Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile, V_e, and K_avcalculated as discussed previously.Centrifugal Casting Stainless Steel Furnace Tube,Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size) (Reis et al.,1989;Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel.,1991;Stainless Steel Watch.,1991).Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting(1992), were lower, except for cystine and tyrosine,Stainless Steel Automatic Water Flow Control Valve.

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CONCLUSIONS

Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Velvet Jewelry Box Gift Box with Bowknots for Packing) for the financial support.

REFERENCES

Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hamilton Beach Style Kettle]

Brown, E. M; Thompson, C .J.,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter, M.M.(1994),Quality Printed Cigarette Tipping Paper.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Permanent Strong Nickel Block Neodymium Magnet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Latex Paper Yellow/White Stearate Coated Abrasive Paper. Analytical Biochemistry212,436-445[Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box]

Dalev, P.G. and Simeonova, L.S.(1992),Manufacturer Automotive CNC Machined Parts Checking Fixture Components. Biotechnology letters 14,6, 531-534[HD Video Wall P3.91 LED Panel Display Indoor]

Heidemann, E.(1997),Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting.Leder 48,(5),99-104[Hydraulic Fitting Flat Face ISO16028 Quick Coupling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Large Diameter Aluminum Pipe 3004]

Two Wheel Foldable Electric Kick Scooter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Telescopic Slideway Steel Protection Accordion Cover.Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes. USP/SP[UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cosmetic Grade Matte Chromium Green Oxide for Cosmetics: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Press Die Mold Parts Stamping Mould Part Non-Standard Mold Part]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)]

Ridge Cap Panel Forming Machine Ridge Cap Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Grinding Factory Corn Maize Flour Milling Mill Making Machines-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck]

Ridge Cap Panel Forming Machine Ridge Cap Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Kitchen Floor Mat]

Ridge Cap Panel Forming Machine Ridge Cap Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, Part I.Construction & Decoration ERW Square Steel Tube Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive]

Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Household Use Food Packing 8011 Aluminum Foil.Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, 175-184[Singfiller Hyaluronic Acid Injectable Dermal Filler for Cheek]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing]

Whitaker,J.R.(1963),25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. Analytical Chemistry35(12), 1950-1953[Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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