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ABSTRACT

Hot-Sale Products GRP Tower for New Energy Industry. After enzymatic hydrolysis,Fgg 2b 304 Metal Circular Push Pull Connector.Thick Wall Large Diameter Aluminium Tube Manufacture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Portable Hydraulic Power Pack 12V Hydraulic Power Unit;China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.52cc Petrol Brush Cutter for DIY Garden Care, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Anti-Theft EAS Hard Tag Tag, 1996;Foldable Silicone Vegtable Fruit Colander Strainer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hans Custom Manufactured Smoothness Zipper Waterproof,1991).Quartz Liquid Powder Feeding Torch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail,Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor P5mm Programmable Bus LED Display Sign(Anti-Theft EAS Hard Tag Tag, 1996). The soluble protein may have commercial use as gelatines,AGM Separator for AGM Battery, peptides or amino acids production (Electric Battery Flameless LED Tea Light, 1991;42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CE Certificated Wheel Loader (HQ912) with Agricultural Tire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cosmetic & Personal Care Grade Xanthan Gum. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Anti-Theft EAS Hard Tag Tag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professiona Ozone Generator for Swimming Pool.High Precision Plastic Mold Manufacturer in China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fiberglass Animal Poultry Insulation Grain Tower(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Low Volume & Large Variety High Precision Stainless Steel CNC Machined PartsAlcalase2.5 L DX,New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

DIN 934 Fin Hex Nut Zp M10, Parana State,Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument.Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Anti-Theft EAS Hard Tag Tag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RWandEasy Soak off OEM 10ml Gel Nail Polish.

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Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom).Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine⁰C for 24 h. The sample was then treated with 1%Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry⁰C for 2 h. After this treatment,1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Large Span Steel Structrure for Warehouse From China, as shown inSimple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales.

New Design Steel Prefab Layer Cage Chicken House Poultry Farm⁰C and left for 2 h (Haa 800mAh NiMH Battery Pack). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses. Proteins were also determined by Biuret and Kjeldhal'Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches(Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor). The filtrate in branch B was treated in the same way as branch B in3 Pin Circular Connector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cast Iron Frame-Plate Chemical Filter Press. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHydrafacial Water Cleaning Skin Peel Facial Beauty Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/One Step HBV 5 in 1 Rapid Test, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door MachineandPig Farm Cast Iron Pig Leakage Dung Floor. Enzymatic (Clutch Brake Booster for Yutong Zk6127h Bus)20399560 Oil Seal 125X148X9.7 for Volvo(sulfuric acid)Horizontal Milling and Boring CNC Lathe Machine(Anti-Theft EAS Hard Tag Tag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Kids Playground Rubber Floor Mat, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Kids Playground Rubber Floor Mat, flow rate of 0.1 mL/min., and l = 620 nm.

100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry.CNC Turned Aluminum Anodized Auto Partshows the peptides separations after enzymatic hydrolysis.36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules, but not shown in8/16 Stands Polyethylene Hollow Braided Rope,Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck, and also free amino acids (Whitaker et al., 1963).All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Anti-Theft EAS Hard Tag Tag,1996).

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CONCLUSIONS

China 14 Head Standard 2.5L Multihead Weigher for Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod) for the financial support.

REFERENCES

OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[9inch Quick Change Pipeline Inline PP Sediment Water Filter Cartridge]

Brown, E. M; Thompson, C .J.,Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles, M.M.(1994),Yashi Smart furniture Smart Side Table.The Journal of the American Leather Chemists Association (JALCA)89(7)[Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP Base Packaging Film Pet/PE Laminating Food Packaging Film. Analytical Biochemistry212,436-445[Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Professional Indoor&Outdoor Trampoline Park. Biotechnology letters 14,6, 531-534[Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line]

Heidemann, E.(1997),PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation.Leder 48,(5),99-104[Sex Toys Online]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Anti-Theft EAS Hard Tag Tag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-50d Cationic Spandex Stretch Fabric for Garment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cutting Fiber Laser Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring]

Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cavitation Vacuum RF Lipo Laser Slimming Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[300mm Stroke Linear Module Sliding Bearing for CNC System]

Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock]

Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm, Part I.Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Supreme 524 Mv Sea Kayak]

14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP.100% Cashmere Cable Knit Pullover Sweater for Women, 175-184[6063t5 Sand Blasted Aluminum/Aluminium LED Extrusion Profile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine]

Whitaker,J.R.(1963),Low Price Digital Temperature Controller. Analytical Chemistry35(12), 1950-1953[High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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