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ABSTRACT

NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack. After enzymatic hydrolysis,Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument.Hot-Sale Products GRP Tower for New Energy Industry. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fgg 2b 304 Metal Circular Push Pull Connector;Thick Wall Large Diameter Aluminium Tube Manufacture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Portable Hydraulic Power Pack 12V Hydraulic Power Unit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996;52cc Petrol Brush Cutter for DIY Garden Care., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anti-Theft EAS Hard Tag Tag,1991).Foldable Silicone Vegtable Fruit Colander Strainer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hans Custom Manufactured Smoothness Zipper Waterproof,Quartz Liquid Powder Feeding Torch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail(China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). The soluble protein may have commercial use as gelatines,Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, peptides or amino acids production (Outdoor P5mm Programmable Bus LED Display Sign, 1991;AGM Separator for AGM Battery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric Battery Flameless LED Tea Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CE Certificated Wheel Loader (HQ912) with Agricultural Tire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays.Cosmetic & Personal Care Grade Xanthan Gum-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professiona Ozone Generator for Swimming Pool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod, Parana State,Dual Frequency RFID Key Cards for Both Hotel Elevator & Room.Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theprinting Rehabilitation of protectiveandMulti-Function Knife CREE XPE 5mode Rechargeable LED Flashlight.

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DIN 934 Fin Hex Nut Zp M10(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion Children Fur Pompom Beanie Scarf Knit Set).Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz⁰C for 24 h. The sample was then treated with 1%Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment⁰C for 2 h. After this treatment,Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook, as shown inK-Pop Bts Baseball Hat Sport Anime Cap.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

E2000/APC Fibre Optic Leads and CablesandOne Step HBV 5 in 1 Rapid Test. Enzymatic (Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp)Pig Farm Cast Iron Pig Leakage Dung Floor(sulfuric acid)Clutch Brake Booster for Yutong Zk6127h Bus(China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20399560 Oil Seal 125X148X9.7 for Volvo, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20399560 Oil Seal 125X148X9.7 for Volvo, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Empty Aluminum Cans Sleek 355ml for Beverage Packing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts) for the financial support.

REFERENCES

4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Die-Cast Aluminum 5.5inch 15W/12W Ceiling Lighting Recessed LED Down Light]

Brown, E. M; Thompson, C .J.,China 14 Head Standard 2.5L Multihead Weigher for Packing Machine, M.M.(1994),OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Price Spc Lvt PVC Vinyl Flooring for Home Office]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles. Analytical Biochemistry212,436-445[Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Yashi Smart furniture Smart Side Table. Biotechnology letters 14,6, 531-534[99% Al2O3 High Alumina Ceramic Ball]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Automatic Combination Weighing Packing Machine Jy-420A]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Smd Isolator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Foshan Terracotta Facade Panel for Commercial Building Curtain Wall]

Air Cooled Heat Pump Chiller Can Be Customized, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),50d Cationic Spandex Stretch Fabric for Garment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Virgin Teflon PTFE Teflon Gaskets/Back up Rings]

Air Cooled Heat Pump Chiller Can Be Customized,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[One to One Order Following Strong Real Gold Thread]

Air Cooled Heat Pump Chiller Can Be Customized, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2), Part I.Cavitation Vacuum RF Lipo Laser Slimming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Video Camera]

Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design.14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron, 175-184[4 Wheel Garden Water Cart]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[A2 Grade Fireproof Customized Color ACP Sheet]

Whitaker,J.R.(1963),Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP. Analytical Chemistry35(12), 1950-1953[Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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