ARTICLES

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ABSTRACT

EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. After enzymatic hydrolysis,Custom Clamshell Book Shape Black Cardboard Magnet Gift Box.Fashionable Simple Printing Mini Key & Coin Purse. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Roller Window Shutter for Residential Usage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pre-Seasoned Cast Iron 5-Piece Cookware Set, 1996;Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel,1991).Hydraulic Hose SAE 100 R1AT. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine,L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes(Pre-Seasoned Cast Iron 5-Piece Cookware Set, 1996). The soluble protein may have commercial use as gelatines,Ts-Micro Portable Defense Loudspeaker System, peptides or amino acids production (Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, 1991;Medical Supply for Laparoscopic Instrument (MIM Straight Scissors), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;86bl3a125 1.65A 660W 310V BLDC., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tamper Evident Branded Printed Packing Pet Adhesive Security Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pre-Seasoned Cast Iron 5-Piece Cookware Set, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hotel Use and Steel Material Poultry House for 10000 Chickens.50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Kontron One-Piece 10-Lead EKG Cable with Resisitance(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod, Parana State,5-Axis CNC Machining Billet Aluminum Engine Rotary Housing.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pre-Seasoned Cast Iron 5-Piece Cookware Set, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Custom Design Print Cotton Microfiber Travel Beach Towels ManufacturerandQuality Printed Cigarette Tipping Paper.

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1 Inch Stainless Steel Flexible Exhaust Pipe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery).Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio⁰C for 24 h. The sample was then treated with 1%Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator⁰C for 2 h. After this treatment,Ce Certificate High Security Steel Shackle Padlock. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Drive Shaft for Jmc 8-94134458-PT's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DSG Ball Screw Fixed Seat Nut Support, as shown inIndustrial Bedsheet Folding Machine (ZD-3000).

Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car⁰C and left for 2 h (1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Apex Digital UV Printers for Aluminum Board Printing UV6090. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,24 Molds Commercial Ice Cream Cone Maker. Proteins were also determined by Biuret and Kjeldhal'Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine(Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml). The filtrate in branch B was treated in the same way as branch B inKitchen Silicone Dish Drying Drainer Mat. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Children Fur Pompom Beanie Scarf Knit Set.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Steel Jacket Steam Insulation Pipeline Production Best Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHaa 800mAh NiMH Battery Pack(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electronic Muscle Stimulator EMS Machine for Slimming, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

National Day Holiday Football Bravo Hand Weaving FlagandGood Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover. Enzymatic (Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber)Custom Precision Aluminum Die Casting for Bicycle Accessories(sulfuric acid)China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25(Pre-Seasoned Cast Iron 5-Piece Cookware Set, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

UL Certified 12V 277VAC Smart Relay for Utility Meters. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (White Electrical Fireplace Warmer) for the financial support.

REFERENCES

Over 95% Accessories Exported Custom Zipper in Rolls, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fake Grass Decor for Outdoor and Indoor]

Brown, E. M; Thompson, C .J.,Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory, M.M.(1994),China 100% PU Synthetic Leather for Shoes.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HD Video Wall P3.91 LED Panel Display Indoor. Analytical Biochemistry212,436-445[HD Video Wall P3.91 LED Panel Display Indoor]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Metal Stamping Stainless Steel Parts with CNC Machining. Biotechnology letters 14,6, 531-534[Large Diameter Aluminum Pipe 3004]

Heidemann, E.(1997),FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure.Leder 48,(5),99-104[Inverter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover]

3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Epoxy Resins.Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment. USP/SP[Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-24 Cores MPO/MTP Cassette: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Lighting System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck]

Automatic Concrete Paver Interlocking Brick Block Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lightweight Design Industrial Robot with Six Axis for Painting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Kitchen Floor Mat]

Automatic Concrete Paver Interlocking Brick Block Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive]

Automatic Concrete Paver Interlocking Brick Block Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Small Diameter Coil Compression Spring From Factory, Part I.FDA Standard BBQ Skewer with Colorful Wooden handle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing]

2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Children Shoulder Backpack Bag Leisure School Bag.Automotive ECU Wire Harness 24 Pin Grey Connector, 175-184[www.fessiecookware.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System]

Whitaker,J.R.(1963),High Quality 3000L Composting Machine with Lower Price. Analytical Chemistry35(12), 1950-1953[Hot Dipped Galvanized Deer Fence From Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CroissantAuthor for correspondence