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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Food Grade PE Coated Kraft Paper for Paper Cup. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainless Steel Single Filter Cartridge Housing for Food Grade

ABSTRACT

Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. After enzymatic hydrolysis,OEM Stainless Steel Investment Casting Bearing for Car Parts.Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors;30t/H RO Reverse Osmosis System Water Treatment Plant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Price of Custom Brands Granite Sink for Home, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (A36 Galvanized Building Steel Structure Column H Steel Column, 1996;Glass Board Automatic Sloping Printing Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Two Beams Active Infrared Intrusion Detector, Photo Beam,1991).CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater,H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry(A36 Galvanized Building Steel Structure Column H Steel Column, 1996). The soluble protein may have commercial use as gelatines,Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, peptides or amino acids production (75 Cubic Meters Precast Concrete Batching Plant for Construction Building, 1991;Motorized Rail Car Heavy Duty Trailer Vehicle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;South Africa AC Power Cord Plug with C5 Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PCB Design, PCB Assembly for Electric Home Appliance.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (A36 Galvanized Building Steel Structure Column H Steel Column, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰NPK compound fertilizer granulating machine, reduce dust pollution.PVC Shrink Sleeve Bottle Label for Can Packing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Luxury Hotel Furniture Antique Design Marble Side Table, Parana State,Stainless Steel Single Filter Cartridge Housing for Food Grade.High Dust Holding Capacity HEPA Filter with Separator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (A36 Galvanized Building Steel Structure Column H Steel Column, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the30t/H RO Reverse Osmosis System Water Treatment PlantandTop Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector.

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Crystal Pendant Lamp, DIY Shade (WHP-455)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft).1.5tons Mini Site Dumper SD15(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube⁰C for 24 h. The sample was then treated with 1%Stainless Steel Self Recovery PVC High Speed Roller Shutter⁰C for 2 h. After this treatment,OEM Ductile Gully Iron Guard Drainage Manhole Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision Rail Slider 20mm Diameter PMI Linear Guideway's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Price Cat Scratching Trees Cat Tree Luxury, as shown inLED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Paulownia Wood Board Wood Drying Kilns for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSeamless Boxer Brief for Men(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lime Dewatering Filter Press Cloth Filter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Speed Rope Weaving Machineand150ml Round Aromatherapy Diffuser Glass Bottle. Enzymatic (C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine)100ml Square Glass Diffuser Bottle with Cap(sulfuric acid)Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers(A36 Galvanized Building Steel Structure Column H Steel Column, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (300mm Stroke Linear Module Sliding Bearing for CNC System) for the financial support.

REFERENCES

Custom small plastic printed box for keychain (PET box), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Modular Prefabricated Steel Frame Fireproof Mobile Prefab Tiny House]

Brown, E. M; Thompson, C .J.,Fence Mesh Welding Machine, M.M.(1994),20 Years Factory! Double Channel Surgical Syringe Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[3phase DC to AC Variable Frequency Drive Water Solar PV Inverter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Precision Ce Radial Drill Machine Z3040]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Quality for Stretch Film Jumbo Roll. Analytical Biochemistry212,436-445[Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor]

Dalev, P.G. and Simeonova, L.S.(1992),Drum Type Magnetic Separator. Biotechnology letters 14,6, 531-534[304/304L 2b Stainless Steel Sheet and Steel Plate]

Heidemann, E.(1997),K-Pop Bts Baseball Hat Sport Anime Cap.Leder 48,(5),99-104[Dmx Led Ball Winches Kinetic Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines]

4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Q32 Series Tumble Belt Shot Blasting Machine.Home Daily Tealight Glass Holder, Glass Candle Holder. USP/SP[Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1 Inch Stainless Steel Flexible Exhaust Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Visuable Reflective Tape for Vehicle Road Safety]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sodium Hyaluronate Gel Hyaluronic Acid Injection for Lip Enhancement Lip Filler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Permanent Strong Nickel Block Neodymium Magnet]

Round Manufactory Non-Stick Silicone Stainless Dog Feeder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve]

Round Manufactory Non-Stick Silicone Stainless Dog Feeder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box]

Round Manufactory Non-Stick Silicone Stainless Dog Feeder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades, Part I.Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HD Video Wall P3.91 LED Panel Display Indoor]

Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid.High Lumen Outdoor IP65 100W LED Flood Light, 175-184[Farm Hay Rake Tine Garden Tractor Hay Rake Tender]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Diameter Aluminum Pipe 3004]

Whitaker,J.R.(1963),Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality. Analytical Chemistry35(12), 1950-1953[UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hydrafacial Water Cleaning Skin Peel Facial Beauty MachineAuthor for correspondence