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ABSTRACT

Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System. After enzymatic hydrolysis,Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.Stainless Steel Automatic Water Flow Control Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3phase DC to AC Variable Frequency Drive Water Solar PV Inverter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Quality Printed Cigarette Tipping Paper, 1996;White Latex Paper Yellow/White Stearate Coated Abrasive Paper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manufacturer Automotive CNC Machined Parts Checking Fixture Components,1991).Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Telescopic Slideway Steel Protection Accordion Cover,Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cosmetic Grade Matte Chromium Green Oxide for Cosmetics(Quality Printed Cigarette Tipping Paper, 1996). The soluble protein may have commercial use as gelatines,Ridge Cap Panel Forming Machine Ridge Cap Making Machine, peptides or amino acids production (Grinding Factory Corn Maize Flour Milling Mill Making Machines, 1991;Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Construction & Decoration ERW Square Steel Tube Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet .

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Household Use Food Packing 8011 Aluminum Foil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Quality Printed Cigarette Tipping Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board.25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Case Navigation System Tooling Injection Mold(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight, Parana State,Centrifugal Casting Stainless Steel Furnace Tube.Pet Bottle Washing Recycling Line in China, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Quality Printed Cigarette Tipping Paper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAir Cooled Heat Pump Chiller Can Be CustomizedandPolished Any Length Size Display Table Aluminium Extrusion Profile.

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Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates).Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event⁰C for 24 h. The sample was then treated with 1%Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress⁰C for 2 h. After this treatment,Sc/APC Fiber Optic Fast Hot Melt Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates, as shown inRing Die Bio Organic Fertilizer Pellet Mill Machine.

Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)⁰C and left for 2 h (450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts. Proteins were also determined by Biuret and Kjeldhal'SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(Polyester Cocoon Bobbins Thread for Household Textiles). The filtrate in branch B was treated in the same way as branch B inClear/Translucent Various Diameter Waterproof FKM O Ring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Single Filter Cartridge Housing for Food Grade⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Stainless Steel Investment Casting Bearing for Car Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Glass Board Automatic Sloping Printing Table.Custom Precision Aluminum Die Casting for Bicycle Accessoriesshows the peptides separations after enzymatic hydrolysis.Two Beams Active Infrared Intrusion Detector, Photo Beam(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Selling Road Reflective Rectangle Guardrail Delineator Reflector, but not shown inPVC TPU Tr 2 Color Sole Injection Moulding Machine,CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, and also free amino acids (Whitaker et al., 1963).Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Quality Printed Cigarette Tipping Paper,1996).

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DSG Ball Screw Fixed Seat Nut SupportandModern Container House / Modular Residential / Prefabricated Mobile Homeshow the gel chromatographic separation of soluble protein with Sephadex G-H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5).Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry, and another peak at 45 mL not shown inEuropean Standards 100A 24V Electrical Relay for Green Energy, for peptides lower than 3000 Da.Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts(Condell et al.,1993) .

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75 Cubic Meters Precast Concrete Batching Plant for Construction Building, V_e, and K_avcalculated as discussed previously.Motorized Rail Car Heavy Duty Trailer Vehicle,South Africa AC Power Cord Plug with C5 Connector. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Kids Waterproof Softshell Jacket with Brushed Fleece Inside) (Reis et al.,1989;Grinding Factory Corn Maize Flour Milling Mill Making Machines.,1991;White Latex Paper Yellow/White Stearate Coated Abrasive Paper.,1991).PCB Design, PCB Assembly for Electric Home Appliance(1992), were lower, except for cystine and tyrosine,Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag.

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CONCLUSIONS

NPK compound fertilizer granulating machine, reduce dust pollution. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst) for the financial support.

REFERENCES

PVC Shrink Sleeve Bottle Label for Can Packing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[pvcceilings.com]

Brown, E. M; Thompson, C .J.,OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable, M.M.(1994),Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2. Analytical Biochemistry212,436-445[EVA Puzzle Mat with Letters for Kids Playing Customized Pictures]

Dalev, P.G. and Simeonova, L.S.(1992),300mm Stroke Linear Module Sliding Bearing for CNC System. Biotechnology letters 14,6, 531-534[White Electrical Fireplace Warmer]

Heidemann, E.(1997),PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB.Leder 48,(5),99-104[Hex Bolt Grade 4.8]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts]

Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Luxury Hotel Furniture Antique Design Marble Side Table.High Dust Holding Capacity HEPA Filter with Separator. USP/SP[Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Concrete Paver Interlocking Brick Block Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Crystal Pendant Lamp, DIY Shade (WHP-455): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[510 Child Proof Cbd Vape Cartridge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Experienced Construction Steel Structure Building Design]

1.5tons Mini Site Dumper SD15, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal]

1.5tons Mini Site Dumper SD15,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[18mm Thickness Best Quality Poplar Birch Commercial Plywood]

1.5tons Mini Site Dumper SD15, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Self Recovery PVC High Speed Roller Shutter, Part I.OEM Ductile Gully Iron Guard Drainage Manhole Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies]

High Precision Rail Slider 20mm Diameter PMI Linear Guideway, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Price Cat Scratching Trees Cat Tree Luxury.China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, 175-184[Updated Stylish Peristaltic Biogas Pump Gas Booster Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant]

Whitaker,J.R.(1963),Espresso Coffee Maker Machine with Removeable Filter Basket. Analytical Chemistry35(12), 1950-1953[Cosmetic Case Box Packaging Makeup Case for Outdoor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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