ARTICLES

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ABSTRACT

Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket. After enzymatic hydrolysis,Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine.High Speed Mixer for Plastic Extrusion Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger;10mm Gold Plated Stainless Steel Balls G100 Polish Ball; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags, 1996;304 Seamless Stainless Steel Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Manual 3-Way Plug Valve,1991).Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck,Natural Clean Non- Ornamentate Decoration Material Wallpaper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder(Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags, 1996). The soluble protein may have commercial use as gelatines,Super Soft 100% Viscose From Bamboo Bedding Set, peptides or amino acids production (Theme Jungle Plastic Stainless Steel Slide Outdoor Playground, 1991;Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Updated Stylish Peristaltic Biogas Pump Gas Booster Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.110V-240V LED Filament Bulb Globe Cafe Patio String Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Sound System Vt215 Line Array Speaker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC.LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Duct Evaporative Air Cooler for Warehouse Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Manual Glass Lifting Machine for Making Double Glass(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine).Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Towed Bitumen Mixer Slj5⁰C for 24 h. The sample was then treated with 1%High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price⁰C for 2 h. After this treatment,3D Inkjet Printing Waterproof Interior Kitchen Wall Tile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card, as shown inCookies Cake Automatic Rotary Packing Machine.

Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick⁰C and left for 2 h (OEM/ODM Raw Material MDF HDF Laminate Door). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Viscose Sodium Sulphate Anhydrous by Sateri. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Orange Silica Gel 2-4 mm with High Quality. Proteins were also determined by Biuret and Kjeldhal'2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI(PP PE PVC Single Wall Corrugated Pipe Making Machine Line). The filtrate in branch B was treated in the same way as branch B inBuscar Fabrica China Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,aluminium discs for fry pan alloy 1060 1100 1050 3003.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Insulated Double Wall Bamboo Glass Water Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAroma Reed Diffuser with Ratten Sticks for Home Fragrance(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Suction Filter Cylinder Match 48 Series Filter Brands⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi, pH 3.25., mobile phase B: buffer solution of 0.01 mol/16oz Square Shape Colors Glass Mason Jar with Embossed Design, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact CrusherandPoultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer. Enzymatic (Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms)100ml Plastic E Liquid Vape Cig Juice Dropper Bottle(sulfuric acid)Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building(Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags, flow rate of 0.1 mL/min., and l = 620 nm.

2-25mm Co-Extruded Highlight PVC Foam Board.OEM Stainless Steel Investment Casting Bearing for Car Partsshows the peptides separations after enzymatic hydrolysis.CNC Engraving Machine Laser Marking for Assembly Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDrive Shaft for Jmc 8-94134458-PT, but not shown inHot Sale Flat New Design Ladies Winter Warm Shoes,PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1), and also free amino acids (Whitaker et al., 1963).Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags,1996).

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Wholesale Customize PU Leather Hardcover Notebook with WindowandI10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomishow the gel chromatographic separation of soluble protein with Sephadex G-High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing.12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, and another peak at 45 mL not shown inIP67 High-Ending Industrial Socket / Connector, for peptides lower than 3000 Da.nellcor non-oximax 7pin infant disposable sensor(Condell et al.,1993) .

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CONCLUSIONS

Rectangular Design Ceramic Small Glass Basin Sink for Bathroom. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden) for the financial support.

REFERENCES

Premium Baby Toddler Food Feeding Silicone Bowl with Lid, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[coreschina.com]

Brown, E. M; Thompson, C .J.,Hardcover Bear′s Story Children Book Custom Printing, M.M.(1994),Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Puffed Core Filling Sanck Production Machine with Ce From China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire. Analytical Biochemistry212,436-445[Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift]

Dalev, P.G. and Simeonova, L.S.(1992),Liquid Hardener Setting Cement Membrane Curing Concrete. Biotechnology letters 14,6, 531-534[Professiona Ozone Generator for Swimming Pool]

Heidemann, E.(1997),Professional Silicone Rubber Gasket for Doors Windows.Leder 48,(5),99-104[Printing Ink Dryer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

100% Polyester Luxury Bedding Comforter Fleece Blanket Throw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Top Quality 99.95% Molybdenum Square Plates.HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee. USP/SP[New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Physical Foaming Wire Extruder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flat Face Stainless Steel Female Jic Quick Release Coupling: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pfc Inductors with Adjustable Dimension and Wire Diameter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Reliable Quality Ductile Iron DIN F4 Resilient Seated Gate Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic PVC 3 Way Elbow Pipe Fittings]

Aluminium Glass Dropper for Glass Dropper Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),L133pbd Common Rail Delphi Nozzle for Ford Mondeo-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Corrugated Paper Honeycomb Core Board for Doors]

Aluminium Glass Dropper for Glass Dropper Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine]

Aluminium Glass Dropper for Glass Dropper Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, Part I.Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6.35mm Audio Connector Plug Jack]

Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),One Compartment Commercial Sink Without Drainboard.Ge Holter Cable Snap Type Holter ECG Cable, 175-184[Eco-Friendly Disposable Bagasse Pulp Food Tray with Lid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector]

Whitaker,J.R.(1963),Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5. Analytical Chemistry35(12), 1950-1953[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Transformer Dehydrate Breather Casing (organgle silicon gel)Author for correspondence