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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PETG Acrylic Sheet for Window, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Frequency Online UPS W/3W-20K, 1996;Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output,1991).14 Head Multihead Weigher for Pork with Screw Feeding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve,PSF 1000 Flat Plate Upper Discharge separator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart(High Frequency Online UPS W/3W-20K, 1996). The soluble protein may have commercial use as gelatines,Powerful Neodymium Pot Magnet Rare Earth Pot Magnet, peptides or amino acids production (CNC Gun Drilling Machine, 1991;Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Large Jewelry Display Box for Ring Necklace Earring Storage Case.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Indoor Tub Customized Shower/Free Standing Shower Enclosure. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Frequency Online UPS W/3W-20K, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Glow in Dark Ceramic Sublimation Mug 11oz.Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Concrete Road Crack-Pouring Machine for Construction(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hand Tools 9PCS Hex Key Set CRV Allen Wrench).Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China OEM Alloy Steel Precision Casting⁰C for 24 h. The sample was then treated with 1%Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport⁰C for 2 h. After this treatment,China Best Quality Hose Crimping Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Galvanized Metal Welded PVC Coated Wire Mesh Fence, as shown in3phase DC to AC Variable Frequency Drive Water Solar PV Inverter.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avXinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Water Cooling Radiator Release Temperature Screw Air Compressor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Injection Mold Maker for Plastic Fighter Toy, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PCBA SMT Equipment PCB Depaneling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price) for the financial support.

REFERENCES

Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[316L 316 Stainless Steel Seamless Round Pipe]

Brown, E. M; Thompson, C .J.,20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, M.M.(1994),304 321 310 310S Welded Decorative Stainless Steel Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS). Analytical Biochemistry212,436-445[Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Dipped Galvanized Deer Fence From Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Amazing Food Processing Machine 2017]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Croissant]

Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Plate Fin Heat Exchanger/Oil Cooler Core-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[100% Durable Flooring Roll for Hotel Office School and Health Project Floor]

Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UL Certified 12V 277VAC Smart Relay for Utility Meters]

Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, Part I.3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Beer Made in China OEM Abv 8.0%Vol Lager Beer]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger]

Whitaker,J.R.(1963),Fashionable Simple Printing Mini Key & Coin Purse. Analytical Chemistry35(12), 1950-1953[8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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