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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Ball Mill, Grinding Mill, Mining Equipment. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Used Tyre Recycling Plant for Reclaimed Rubber Production

ABSTRACT

Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c. After enzymatic hydrolysis,Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head.Medical Ce Approved Bp Machine Blood Pressure Monitor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910;2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor, 1996;Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Electric Chain Saw Cutting Machine Price,1991).A2 Grade Fireproof Customized Color ACP Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Approved Cheap Medical Disposable Electrical Infusion Pump,Fashion Party Decoration Classic Wine Glass Accessories Jewelry,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides(Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor, 1996). The soluble protein may have commercial use as gelatines,32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores, peptides or amino acids production (Galvanized Steel Barbed Wire Rope 8mm, 1991;CNC Aluminum Laser Milling Machinery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Porosity Zirconia Zro2 Foam Filter for Foundry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids.Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine).Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale⁰C for 24 h. The sample was then treated with 1%Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat⁰C for 2 h. After this treatment,Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Car Seat Belt Shoulder Protector 80438's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder, as shown inCoreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine.

Professional Full Automatic Machine for Making Wood Pellets⁰C and left for 2 h (Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 20PCS Colorful Makeup Brush Set. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy. Proteins were also determined by Biuret and Kjeldhal'PE Plastic Road Safety Barricade Caution Tape(Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck). The filtrate in branch B was treated in the same way as branch B inDirt Bikes For $200. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Screw Barrel for PVC PPR Pipe Extrusion.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGold Open Metal Short Crocodile Wallet for Girl Women Lady(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pond Self Priming Booster suction  Water Pump with Pressure Sensor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/95g 3D Tooth Stain Remover Whitelock Toothpaste, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Kitchen Floor MatandGrass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats. Enzymatic (Al/Pet/Al Double Sides for Aluminium Flexible Air Duct)European Standards 100A 24V Electrical Relay for Green Energy(sulfuric acid)Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal(Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Canvas Shoulder Weekend Shopping Big Bag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Canvas Shoulder Weekend Shopping Big Bag, flow rate of 0.1 mL/min., and l = 620 nm.

High Visuable Reflective Tape for Vehicle Road Safety.5.5 to 7.5kw AC Frequency Inverter for Motor AC Driveshows the peptides separations after enzymatic hydrolysis.Made in China Aluminum Extrusion Window and Door Profile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing, but not shown inRosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System,Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc, and also free amino acids (Whitaker et al., 1963).Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor,1996).

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Croissantand100% Durable Flooring Roll for Hotel Office School and Health Project Floorshow the gel chromatographic separation of soluble protein with Sephadex G-304/304L 2b Stainless Steel Sheet and Steel Plate.Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier, and another peak at 45 mL not shown inUL Certified 12V 277VAC Smart Relay for Utility Meters, for peptides lower than 3000 Da.Plastic Injection for OEM Product(Condell et al.,1993) .

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CONCLUSIONS

Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Electric PCB Circuit Board with High Quality) for the financial support.

REFERENCES

Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[358 Welded Wire Mesh Fence]

Brown, E. M; Thompson, C .J.,Portable Ink Date Printing Machine for Plastic Bag, M.M.(1994),High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0.The Journal of the American Leather Chemists Association (JALCA)89(7)[Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashionable Clothing Garment Paper Swing Tags (ST-01)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz. Analytical Biochemistry212,436-445[Telescopic Slideway Steel Protection Accordion Cover]

Dalev, P.G. and Simeonova, L.S.(1992),Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods. Biotechnology letters 14,6, 531-534[Galvanized Steel Barbed Wire Rope 8mm]

Heidemann, E.(1997),Best Items Gh/Genatropin with Naked Vial.Leder 48,(5),99-104[New design for Arabric Man Slipper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Wine Carrier Bag Gift Box Tote]

Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Size Gel Nail Polish Set Boxes with Sponge.High Precision Ce Radial Drill Machine Z3040. USP/SP[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[304 Seamless Stainless Steel Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carton Making Machine Corrugated Box Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory]

Dual Frequency RFID Key Cards for Both Hotel Elevator & Room, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Canvas Casual Men Shoes]

Dual Frequency RFID Key Cards for Both Hotel Elevator & Room,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[One Step HBV 5 in 1 Rapid Test]

Dual Frequency RFID Key Cards for Both Hotel Elevator & Room, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument, Part I.Hot-Sale Products GRP Tower for New Energy Industry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Kraft Paper Laminated Aluminum Foil]

Fgg 2b 304 Metal Circular Push Pull Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Thick Wall Large Diameter Aluminium Tube Manufacture.Portable Hydraulic Power Pack 12V Hydraulic Power Unit, 175-184[ALP0810]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)]

Whitaker,J.R.(1963),China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. Analytical Chemistry35(12), 1950-1953[Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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