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ABSTRACT

Automatic Waste Paper Stripper. After enzymatic hydrolysis,Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type).Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (99% Purity Caustic Soda Flakes From Direct Manufacture on Sale, 1996;Bwb-306 Custom Printing Outdoor Sports Wristband., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Irregular Shape Hologram Label Printing,1991).High Speed Rope Weaving Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill,Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys(99% Purity Caustic Soda Flakes From Direct Manufacture on Sale, 1996). The soluble protein may have commercial use as gelatines,Funny Kids Soft Play Maze Big Slide Indoor Soft Playground, peptides or amino acids production (Large Prototype CNC Transmission Assembly Tractor Machinery Part, 1991;China Durable 25kg 50kg Red PP Woven Bag Sack, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Recorder Sound Box for Musical Box & Plush Toys.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fake Fish Luminescent Tropical Fish Fish Tank Decoration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (99% Purity Caustic Soda Flakes From Direct Manufacture on Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine.High Pressure Die Casting Aluminum Die Casting Gear Box Housing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Galvanized Bar Grating(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe).Ring Die Bio Organic Fertilizer Pellet Mill Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Dipped Galvanized Deer Fence From Factory⁰C for 24 h. The sample was then treated with 1%High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce⁰C for 2 h. After this treatment,Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cheap Customized Aluminium Sand Casting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper, as shown inChina Xr150L/Pulsar 180 Engine Parts Cylinder Kit.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100% Hand-Painted Canvas Oil Painting Wall Art for Trees. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactort Price Cattle Beef Slaughtering Equipment for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Definition Outdoor Video Advertising LED Billboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper.Pet Glitter Powder for Arts and Crafts/Decoration /Cosmeticshows the peptides separations after enzymatic hydrolysis.33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCe TUV SGS BV Certificate CNC Router with Rotary Fixture China, but not shown inFence Mesh Welding Machine,China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons, and also free amino acids (Whitaker et al., 1963).OEM Composite Machining Phenolic Titanium Parts Products Supplier. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (99% Purity Caustic Soda Flakes From Direct Manufacture on Sale,1996).

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CONCLUSIONS

FTTH Optical Network Terminal for Gpon ONU and CATV. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Portable Air Water Purifier with Disinfection and Detoxification) for the financial support.

REFERENCES

OEM Customized Polyester Cotton National President Election Supporters Printed Scarf, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automobile Spare Part for Transportation]

Brown, E. M; Thompson, C .J.,D8tc High Performance 3-Electrode Motorcycle Spark Plug, M.M.(1994),Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads.The Journal of the American Leather Chemists Association (JALCA)89(7)[SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Power Solar Inverter with Full Power 3000W Converter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging. Analytical Biochemistry212,436-445[Double Jacketed Cooking Kettle Electric Jacket Boiler]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag. Biotechnology letters 14,6, 531-534[Fashion Party Decoration Classic Wine Glass Accessories Jewelry]

Heidemann, E.(1997),19m Mobile LED Display Billboard for Advertising Screens.Leder 48,(5),99-104[Shockproof Sleeve Flip Cover Bag for iPad 10 Inch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[One Compartment Commercial Sink Without Drainboard]

Screw Dosing Feeder Used for Chicken Feed Plant, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nice Appearance Brass Metal Sheet Stamping.Hot Sale Starter Motor Testing Equipment for Truck, Bus. USP/SP[High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Frame Mount Highway Pegs Enclosure Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Home Machine 2019 New Commercial Treadmill Gym Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[11-12"]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Retail Supermarket Display Refrigerator Supermarket Beverage Showcase]

New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Permanent Magnetic Lifter]

New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Agriculture Indoor Vegetables and Fruits Planting Greenhouse]

New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Clothing Hard Tag Security Tag for Supermarket/Retail Store, Part I.150 Ton Hydraulic Press Machine for Cooking Pot Making. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts]

Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Flat New Design Ladies Winter Warm Shoes.Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves, 175-184[Amazon Pet Accessories Dog Bed Pet Luxury Soft Pet Beds for Dogs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flat Face Stainless Steel Female Jic Quick Release Coupling]

Whitaker,J.R.(1963),OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. Analytical Chemistry35(12), 1950-1953[52cc Gasoline Paddy Cutter Two Handle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Brightness 100lm/W 45W CREE COB LED Track LightAuthor for correspondence