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ABSTRACT

PVC Shrink Sleeve Bottle Label for Can Packing. After enzymatic hydrolysis,OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable.Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Luxury Hotel Furniture Antique Design Marble Side Table, 1996;High Dust Holding Capacity HEPA Filter with Separator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Crystal Pendant Lamp, DIY Shade (WHP-455),1991).1.5tons Mini Site Dumper SD15. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube,Stainless Steel Self Recovery PVC High Speed Roller Shutter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Ductile Gully Iron Guard Drainage Manhole Cover(Luxury Hotel Furniture Antique Design Marble Side Table, 1996). The soluble protein may have commercial use as gelatines,High Precision Rail Slider 20mm Diameter PMI Linear Guideway, peptides or amino acids production (Factory Price Cat Scratching Trees Cat Tree Luxury, 1991;China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Espresso Coffee Maker Machine with Removeable Filter Basket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Low Profile Sp-Level Linear Rail for 3D Printer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Luxury Hotel Furniture Antique Design Marble Side Table, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Xr150L/Pulsar 180 Engine Parts Cylinder Kit.High Quality Paulownia Wood Board Wood Drying Kilns for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Seamless Boxer Brief for Men(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

150ml Round Aromatherapy Diffuser Glass Bottle, Parana State,NPK compound fertilizer granulating machine, reduce dust pollution.C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Luxury Hotel Furniture Antique Design Marble Side Table, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWater Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe FittingsandTear Strip Tape.

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100ml Square Glass Diffuser Bottle with Cap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10).Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts⁰C for 24 h. The sample was then treated with 1%Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve⁰C for 2 h. After this treatment,17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reusable CAT6 UTP RJ45 8P8C Modular Plug's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, as shown inPower 4 Leg Transmission Tower From Qingdao Wuxiao Group.

Good Quality OEM Stainless Steel Casting Rings⁰C and left for 2 h (Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customizable Logo Plastic Safety Walking Reflectors Chinese Factory. Proteins were also determined by Biuret and Kjeldhal'Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle(Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine). The filtrate in branch B was treated in the same way as branch B inElectronic Device Aluminum Extruded Enclosure for Amplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flame Retardant FRP/GRP Round Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Psa Oxygen Generator for Industry (ISO9001, CE). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom small plastic printed box for keychain (PET box), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fence Mesh Welding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Home Daily Tealight Glass Holder, Glass Candle Holder.Industrial Steel Structure Mezzanine Rack Platform System/Book Shelfshows the peptides separations after enzymatic hydrolysis.Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz, but not shown inDual Frequency RFID Key Cards for Both Hotel Elevator & Room,Round Manufactory Non-Stick Silicone Stainless Dog Feeder, and also free amino acids (Whitaker et al., 1963).Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Luxury Hotel Furniture Antique Design Marble Side Table,1996).

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Factort Price Cattle Beef Slaughtering Equipment for SaleandYashi Smart furniture Smart Side Tableshow the gel chromatographic separation of soluble protein with Sephadex G-15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades.Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors, and another peak at 45 mL not shown inReusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter, for peptides lower than 3000 Da.Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)(Condell et al.,1993) .

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CONCLUSIONS

Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies) for the financial support.

REFERENCES

Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[14GSM Bleached& Unbleached Cigarette Paper Hemp Paper with Filters]

Brown, E. M; Thompson, C .J.,Full Automatic Combination Weighing Packing Machine Jy-420A, M.M.(1994),Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hydraulic Hose SAE 100 R1AT]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tfl Blue Rubber Transmission Belts/Automotive Spare Part. Analytical Biochemistry212,436-445[Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service]

Dalev, P.G. and Simeonova, L.S.(1992),1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China. Biotechnology letters 14,6, 531-534[Metal Tin with Clear Lid]

Heidemann, E.(1997),Flat Wedge Wire Panel China Factory.Leder 48,(5),99-104[Car Factory Passive Keyless Entry]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Orange Silica Gel 2-4 mm with High Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Spray Masking Tape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip]

CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Design Disposable Trocars for Laparoscopic Surgery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hans Custom Manufactured Smoothness Zipper Waterproof]

CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck]

CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Disposable Medical Combined Bacterial Viral Hme Filter, Part I.Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Steel Track for Sectional Garage Door]

Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb.High Efficiency Pineapple Canning Plant, 175-184[Honed Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fireproof WPC Indoor Decorative Wall Panel]

Whitaker,J.R.(1963),Embossed Pattern PU Leather Classic Mens Wallet. Analytical Chemistry35(12), 1950-1953[52cc Petrol Brush Cutter for DIY Garden Care]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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