ARTICLES

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ABSTRACT

CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts. After enzymatic hydrolysis,EPE Foam Package Extruding Production Line.3 Layer Film Blowing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Density Graphite Crucible for Melting Metal, 1996;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine,1991).Medical Supplier Non Woven 3ply Earloop Disposable Face Mask. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)(High Density Graphite Crucible for Melting Metal, 1996). The soluble protein may have commercial use as gelatines,LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, peptides or amino acids production (Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine, 1991;New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.19gauge Galvanized Twin Binding Wire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Density Graphite Crucible for Melting Metal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6.35mm Audio Connector Plug Jack.Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6).Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.⁰C for 24 h. The sample was then treated with 1%Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover⁰C for 2 h. After this treatment,Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle, as shown inHandheld Andriod POS with Thermal Printer.

Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11⁰C and left for 2 h (Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Soap Bag in Box Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting. Proteins were also determined by Biuret and Kjeldhal'High Performance Alcohol Pad Making Machine(High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts). The filtrate in branch B was treated in the same way as branch B inDongfeng Crane Boom Hydraulic Lorry 10 Ton Knuckle Telescopic Boom Truck Mounted Crane for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ss304 Sanitary Welded Butterfly Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnti-Abrasion PVC Artificial Leather for Car Seat (128#)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Economical Check Weigher with High Speed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Electric DC 24V Geared Motor for Auto DoorsandHigh Quality Small Tea Bag Packing Machine Price. Enzymatic (Carbon Dioxide Gas with 50L Seamless Cylinder)UV8d Wouxun High Gain Antenna Dual Band Antenna(sulfuric acid)Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire(High Density Graphite Crucible for Melting Metal, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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Automatic Soap Bag in Box Packing MachineandNew Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountainshow the gel chromatographic separation of soluble protein with Sephadex G-Good Quality CZ Purlin One Machine Roll Forming Machine.Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM, and another peak at 45 mL not shown inIndustrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine, for peptides lower than 3000 Da.Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools(Condell et al.,1993) .

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Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China, V_e, and K_avcalculated as discussed previously.Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box,Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch) (Reis et al.,1989;Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine.,1991;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine.,1991).Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire(1992), were lower, except for cystine and tyrosine,Modern Container House / Modular Residential / Prefabricated Mobile Home.

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CONCLUSIONS

100% Polyester Luxury Bedding Comforter Fleece Blanket Throw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight, M.M.(1994),Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine. Analytical Biochemistry212,436-445[GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Mixer for Plastic Extrusion Line. Biotechnology letters 14,6, 531-534[Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar]

Heidemann, E.(1997),High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger.Leder 48,(5),99-104[Cast Iron or Stainless Steel Lug Dual Plate Wafer Check Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Clip on Wire Shelving Data Strip]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Wave Spot Soldering Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Neodymium Magnet Tweeter]

304 Seamless Stainless Steel Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Manual 3-Way Plug Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[304 321 310 310S Welded Decorative Stainless Steel Pipe]

304 Seamless Stainless Steel Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine]

304 Seamless Stainless Steel Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, Part I.Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nice Appearance Brass Metal Sheet Stamping]

Natural Clean Non- Ornamentate Decoration Material Wallpaper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder.Super Soft 100% Viscose From Bamboo Bedding Set, 175-184[Jewellery Rfid Tag Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[300W-2kw Solar Panel and Wind Turbine Hybrid System]

Whitaker,J.R.(1963),Theme Jungle Plastic Stainless Steel Slide Outdoor Playground. Analytical Chemistry35(12), 1950-1953[1054 FRP Pressure Vessel for Water Softener & Water Treatment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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