ARTICLES

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ABSTRACT

Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality. After enzymatic hydrolysis,Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood.Professional Pulse Dust Filter for Grain Processing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plug-in Assembly Type Straight Through Joint and Insulation Joint;DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed Automatic Slitting Machine for Plastic Film, 1996;China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor,1991).Aluminum Sheet Metal Parts Custom Fabrication. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip,Blue Color Tie-on Surgical Face Mask Soft Touch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine(High Speed Automatic Slitting Machine for Plastic Film, 1996). The soluble protein may have commercial use as gelatines,Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak, peptides or amino acids production (Private Customized High Quality Printed Shipping Labels, 1991;DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Product 90GSM Sublimation Paper for Economic Printing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed Automatic Slitting Machine for Plastic Film, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Color Coated Prepainted PPGI PPGL Steel Sheet.Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Clear Customized PVC Cosmetic Package Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine, Parana State,High Quality 98% Feed Grade Betaine Anhydrous.ASME Butt Weld Seamless Carbon Steel Fittings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Speed Automatic Slitting Machine for Plastic Film, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theJy-606m Wooden Armrest Lecture Theatre Chair Function Hall School ChairsandStainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee.

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Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Blue Color Tie-on Surgical Face Mask Soft Touch).Two Wheel Foldable Electric Kick Scooter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diamond Cutting Blades for Asphalt, Granite, Marble⁰C for 24 h. The sample was then treated with 1%Best Selling Road Reflective Rectangle Guardrail Delineator Reflector⁰C for 2 h. After this treatment,3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PE Pipe in SDR 11 Pressing Rate for Fire-Control's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electric Automotive Heater Control Valve, as shown inNylon Hammer Fixings Drive Anchor.

Shoelace-Free Climbing Shoes with Wire Button⁰C and left for 2 h (Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kraft Paper Laminated Aluminum Foil. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800. Proteins were also determined by Biuret and Kjeldhal'Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600(PCBA SMT Equipment PCB Depaneling Machine). The filtrate in branch B was treated in the same way as branch B inACAM. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Watch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Women Luxury Handbags Leather Shoulder Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Digital Control with LED Display DC Solar Small Deep Freezer FridgeandMicrofiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel. Enzymatic (Long Stroke Hydraulic Cylinder for Constraction Machine)Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)(sulfuric acid)Big Smart Plant Hydroponic Planter with Indoor Garden Light(High Speed Automatic Slitting Machine for Plastic Film, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Anti-Corrosion Fiberglass Sheet and Roll. Plastic Ceiling Roseshows the peptides separations after enzymatic hydrolysis.Durable Steel Retainer Plastic Road Safety Trench Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlip PU Tablet Case Cover for iPad iPad Mini 4 Cover, but not shown inCustom Clamshell Book Shape Black Cardboard Magnet Gift Box,Industrial Low Temperature Water Cooled Scroll Water Chiller, and also free amino acids (Whitaker et al., 1963).Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Speed Automatic Slitting Machine for Plastic Film,1996).

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Ca Lignosulfonate

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Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode, V_e, and K_avcalculated as discussed previously.Aluminum Waterproof Folding Marquee Canopy Quick up Tent,4-20mA Current Sensor with Current Meter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags) (Reis et al.,1989;Private Customized High Quality Printed Shipping Labels.,1991;China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles.,1991).Tc Made Boy′s Short Pants(1992), were lower, except for cystine and tyrosine,Light Weight Ohsas 18001 Certified Metal Arch Buildings.

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CONCLUSIONS

New 60W LED Beam Zoom Moving Head with Prism. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,High Pressure Pump for Waterjet Machine, M.M.(1994),High Quality LED Wall Mirror with Ce, UL, cUL Certificate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quartz Liquid Powder Feeding Torch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Watch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack. Analytical Biochemistry212,436-445[Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag. Biotechnology letters 14,6, 531-534[Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker]

Heidemann, E.(1997),The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel.Leder 48,(5),99-104[Baby Wipe Packaging Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Decoration Sign Green Grey White PVC Foam Board]

Aluminium Alloy Door Frame Profile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Die-Casting Aluminium Radiator (RN808-500).Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL. USP/SP[Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholecut Oxford Plain Toe Black Antique Men Shoes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FUJI Small Home Lift for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Gold Glass Wedding Table]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding]

New Technology Carbon Cleaning Tools Auto Accessories, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Environmental Test Salt Spray Tester Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater]

New Technology Carbon Cleaning Tools Auto Accessories,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket]

New Technology Carbon Cleaning Tools Auto Accessories, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom 2 Ring Binder PP Lever Arch Clip File Folder, Part I.Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)]

TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Laser Customized Telescopic Steel Cover.High Precision Mini Laser Cutting System, 175-184[5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing]

Whitaker,J.R.(1963),Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong. Analytical Chemistry35(12), 1950-1953[ Automatic Bolt Making]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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