ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CNC Gun Drilling Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products

ABSTRACT

High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide. After enzymatic hydrolysis,Large Jewelry Display Box for Ring Necklace Earring Storage Case.Indoor Tub Customized Shower/Free Standing Shower Enclosure. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Glow in Dark Ceramic Sublimation Mug 11oz;Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996;Supermarket Combined Island Chest Freezer Commercial Refrigerator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price,1991).High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Clip on Wire Shelving Data Strip,Custom Black and White Hard Laptop Case Travel Suitcase,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Concrete Road Crack-Pouring Machine for Construction(22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996). The soluble protein may have commercial use as gelatines,Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck, peptides or amino acids production (China OEM Alloy Steel Precision Casting, 1991;Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Best Quality Hose Crimping Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Metal Welded PVC Coated Wire Mesh Fence. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand.720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Precision iPhone Cases Fiber Laser Marking Machine, Parana State,Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products.Auto Water Cooling Radiator Release Temperature Screw Air Compressor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePE Plastic Road Safety Barricade Caution TapeandGlass Board Automatic Sloping Printing Table.

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Injection Mold Maker for Plastic Fighter Toy(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket).PCBA SMT Equipment PCB Depaneling Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%HDPE Pipe for Water Supply (OD: 16mm-800mm)⁰C for 24 h. The sample was then treated with 1%Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs⁰C for 2 h. After this treatment,2019 Male & Female Stainless Steel Quick Coupling. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Digital Floating Baby Care Products Frog Bath Design Thermometer, as shown in99% Purity Caustic Soda Flakes From Direct Manufacture on Sale.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av18mm Thickness Best Quality Poplar Birch Commercial Plywood(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nylon Hammer Fixings Drive Anchor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Electrolyte Effervescent Tablet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Custom Clamshell Book Shape Black Cardboard Magnet Gift Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC) for the financial support.

REFERENCES

Fashionable Simple Printing Mini Key & Coin Purse, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kz-32/19 Double Button Heavy Duty Hang Pneumatic Steel Strapping Tool]

Brown, E. M; Thompson, C .J.,CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch, M.M.(1994),Kids Folding Play Mat Kids Play Room Floor Mat.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Single PC Sachet Bag Wet Tissue Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[NBR EPDM Neoprene Silicone Rubber Sheet for Seal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Roller Window Shutter for Residential Usage. Analytical Biochemistry212,436-445[Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)]

Dalev, P.G. and Simeonova, L.S.(1992),Pre-Seasoned Cast Iron 5-Piece Cookware Set. Biotechnology letters 14,6, 531-534[Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid]

Heidemann, E.(1997),Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts.Leder 48,(5),99-104[F00vc01377 Bosch Common Rail Spare Parts Valve F 00V C01 377 Atomatic Valve Foovc01377 for 0445110362 0445110363]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Durable Steel Retainer Plastic Road Safety Trench Cover]

Powerful Neodymium Pot Magnet Rare Earth Pot Magnet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel.Hydraulic Hose SAE 100 R1AT. USP/SP[High Precision Rail Slider 20mm Diameter PMI Linear Guideway]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[A4 Document Leather File Folder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ IV Fluid Set with Precision Filter & Luer Lock]

L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Sound System Vt215 Line Array Speaker]

L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Logo Printing Cheap Gold Custom Paper Clip]

L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ts-Micro Portable Defense Loudspeaker System, Part I.Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[EPE Foam Package Extruding Production Line]

Medical Supply for Laparoscopic Instrument (MIM Straight Scissors), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),86bl3a125 1.65A 660W 310V BLDC.Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, 175-184[Depth Throat Swing Beam Cutting Machine and Blades for Iron Sheet in China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth]

Whitaker,J.R.(1963),530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle. Analytical Chemistry35(12), 1950-1953[Outdoor P5mm Programmable Bus LED Display Sign]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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