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ABSTRACT

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Key words;PE Pipe in SDR 11 Pressing Rate for Fire-Control;Electric Automotive Heater Control Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shoelace-Free Climbing Shoes with Wire Button, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Kraft Paper Laminated Aluminum Foil, 1996;China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600,1991).Stainless Steel Watch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts,150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine(Kraft Paper Laminated Aluminum Foil, 1996). The soluble protein may have commercial use as gelatines,CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal, peptides or amino acids production (Women Luxury Handbags Leather Shoulder Bag, 1991;P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Long Stroke Hydraulic Cylinder for Constraction Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Neoprene Protective Elbow Protector Brace Elbow Support (NS0012). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Kraft Paper Laminated Aluminum Foil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Big Smart Plant Hydroponic Planter with Indoor Garden Light.Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Anti-Corrosion Fiberglass Sheet and Roll(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Pressure Pump for Waterjet Machine⁰C and left for 2 h (Super Handy Home & Office & Hotel Facial Tissue Paper). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality LED Wall Mirror with Ce, UL, cUL Certificate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack. Proteins were also determined by Biuret and Kjeldhal'Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag(Automatic Electric Hydraulic Metal Bending Machine). The filtrate in branch B was treated in the same way as branch B inFluorescent Green Black Arrow Reflective Tape. The final soluble protein shown were used for peptides separations using gel permeation chromatography,The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Die-Casting Aluminium Radiator (RN808-500). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Technology Carbon Cleaning Tools Auto Accessories⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Environmental Test Salt Spray Tester Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom 2 Ring Binder PP Lever Arch Clip File Folder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)) for the financial support.

REFERENCES

Stainless Steel Automatic Water Flow Control Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sex Toy Oral Sex Doll for Man Silicone Dolls Man Toy]

Brown, E. M; Thompson, C .J.,Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0, M.M.(1994),Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Empty Aluminum Cans Sleek 355ml for Beverage Packing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aroma Reed Diffuser with Ratten Sticks for Home Fragrance]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3phase DC to AC Variable Frequency Drive Water Solar PV Inverter. Analytical Biochemistry212,436-445[Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber]

Dalev, P.G. and Simeonova, L.S.(1992),Quality Printed Cigarette Tipping Paper. Biotechnology letters 14,6, 531-534[Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803]

Heidemann, E.(1997),White Latex Paper Yellow/White Stearate Coated Abrasive Paper.Leder 48,(5),99-104[Jm300 Domestic Use Round Bowl Kitchen Sink Wholesale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multifunction Copper Busbar Processing for Aluminum Material]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Telescopic Slideway Steel Protection Accordion Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nickel Plating Cloisonne Gold Enamel Metal Medal (068)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0.12-2.0mm*600-1250mm Prepainted Color PPGL/PPGI Steel Coil for Southeast Asia]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic]

Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cosmetic Grade Matte Chromium Green Oxide for Cosmetics-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube]

Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[86 Type WiFi Smart Touch Switch Controll by Tuya APP]

Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ridge Cap Panel Forming Machine Ridge Cap Making Machine, Part I.Grinding Factory Corn Maize Flour Milling Mill Making Machines. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Soccer Field Artificial Grass Cutting Machine for Installation]

Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Construction & Decoration ERW Square Steel Tube Manufacturer.Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , 175-184[C11000 C12200 C10200 Copper Sheet C11000 C12200]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100ml Square Glass Diffuser Bottle with Cap]

Whitaker,J.R.(1963),Household Use Food Packing 8011 Aluminum Foil. Analytical Chemistry35(12), 1950-1953[Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Frequency Circular Vibrating ScreenAuthor for correspondence