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ABSTRACT

Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear. After enzymatic hydrolysis,FTTH Optical Network Terminal for Gpon ONU and CATV.OEM Customized Polyester Cotton National President Election Supporters Printed Scarf. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag, 1996;19m Mobile LED Display Billboard for Advertising Screens., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nice Appearance Brass Metal Sheet Stamping,1991).Hot Sale Starter Motor Testing Equipment for Truck, Bus. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Home Machine 2019 New Commercial Treadmill Gym Equipment,New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line(Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag, 1996). The soluble protein may have commercial use as gelatines,Clothing Hard Tag Security Tag for Supermarket/Retail Store, peptides or amino acids production (150 Ton Hydraulic Press Machine for Cooking Pot Making, 1991;Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Flat New Design Ladies Winter Warm Shoes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine.2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IP67 High-Ending Industrial Socket / Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box, Parana State,Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle.Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock PouchandViscose Sodium Sulphate Anhydrous by Sateri.

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0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electronic Muscle Stimulator EMS Machine for Slimming).Bgf-800 Pet Dry Laminating Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse⁰C for 24 h. The sample was then treated with 1%Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging⁰C for 2 h. After this treatment,Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polyester Cocoon Bobbins Thread for Household Textiles, as shown inQuality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale.

Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood⁰C and left for 2 h ( 15mm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.HAIRUI 850A+ 600W SMD Rework Soldering Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Oval Shape Cast Iron Dutch Oven. Proteins were also determined by Biuret and Kjeldhal'White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara(Clutch Brake Booster for Yutong Zk6127h Bus). The filtrate in branch B was treated in the same way as branch B in22213 Bearing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Pipe Fitting 304 Cross of 3/4 Inch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Performance Fresh Air Handling Unit for Marine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Community IC Access Card Induction ID Time Card Smart Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Packing Machine Biscuit Packing Machine with Auto Feeder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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P10.4 Outdoor LED Transparent Display Screen Glass Curtain ScreenandNight Vision Wide HD Waterproof Car Side View Camera for Busshow the gel chromatographic separation of soluble protein with Sephadex G-Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes.Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml, and another peak at 45 mL not shown in10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, for peptides lower than 3000 Da.5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers(Condell et al.,1993) .

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Automatic Single PC Sachet Bag Wet Tissue Machine, V_e, and K_avcalculated as discussed previously.Aluminum Road Stud,Lab Water Instruments/Lab Water Purification System Micro. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles) (Reis et al.,1989;150 Ton Hydraulic Press Machine for Cooking Pot Making.,1991;19m Mobile LED Display Billboard for Advertising Screens.,1991).Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia(1992), were lower, except for cystine and tyrosine,Adult Neoprene Sport Summer Beach Aqua Swimming Shoes.

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CONCLUSIONS

Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price) for the financial support.

REFERENCES

Lightweight Compact 70d Ripstop Nylon Parachute Hammock, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Beautiful Trombone]

Brown, E. M; Thompson, C .J.,0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation, M.M.(1994),China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106).The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Double Bowl Undermount for Stainless Steel Kitchen Sink. Analytical Biochemistry212,436-445[Factory Wholesale Reach Standard Home and Office Furniture Fitting]

Dalev, P.G. and Simeonova, L.S.(1992),Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport. Biotechnology letters 14,6, 531-534[Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine]

Heidemann, E.(1997),Bitumen Waterproof Membrane Minerla Surface Waterproof Material.Leder 48,(5),99-104[PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-2)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Silver Anodized Aluminium 135 Degree Angle Bracket]

Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),M10 3D Solid Non-Woven Folded Mask Body Making Machine.Pfc Inductors with Adjustable Dimension and Wire Diameter. USP/SP[Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Drum Rotator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Permanent Magnetic Lifter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Lid Press Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ce& FDA Certified Organic Extra Virgin Cold Pressed Coconut Oil in Bulk Private Label]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets]

Large Diameter Aluminum Pipe 3004, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Unfolding Grocery Push Carts]

Large Diameter Aluminum Pipe 3004,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bwb-306 Custom Printing Outdoor Sports Wristband]

Large Diameter Aluminum Pipe 3004, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater, Part I.Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply]

Wholesale Logo Printing Cheap Gold Custom Paper Clip, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;.Non - Smell Fiber Reinforced PVC Garden Hose, 175-184[920mm Hot Forming Large Dia Steel Pipe Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Whitaker,J.R.(1963),PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet. Analytical Chemistry35(12), 1950-1953[Easy Soak off OEM 10ml Gel Nail Polish]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Yoga TowelAuthor for correspondence