ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cosmetic & Personal Care Grade Xanthan Gum. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Professiona Ozone Generator for Swimming Pool

ABSTRACT

High Precision Plastic Mold Manufacturer in China. After enzymatic hydrolysis,Fiberglass Animal Poultry Insulation Grain Tower.Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DIN 934 Fin Hex Nut Zp M10, 1996;Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz,1991).Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine,Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook(DIN 934 Fin Hex Nut Zp M10, 1996). The soluble protein may have commercial use as gelatines,Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size, peptides or amino acids production (Large Span Steel Structrure for Warehouse From China, 1991;New Design Steel Prefab Layer Cage Chicken House Poultry Farm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DIN 934 Fin Hex Nut Zp M10, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box.Cast Iron Frame-Plate Chemical Filter Press-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pig Farm Cast Iron Pig Leakage Dung Floor, Parana State,Professiona Ozone Generator for Swimming Pool.Clutch Brake Booster for Yutong Zk6127h Bus, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (DIN 934 Fin Hex Nut Zp M10, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKids Playground Rubber Floor MatandHeavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Optical PCB Bar Connector for Jst Scn 2.5mm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEmpty Aluminum Cans Sleek 355ml for Beverage Packing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China 14 Head Standard 2.5L Multihead Weigher for Packing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (One Step HBV 5 in 1 Rapid Test) for the financial support.

REFERENCES

Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[tonyearchem.com]

Brown, E. M; Thompson, C .J.,Polished Any Length Size Display Table Aluminium Extrusion Profile, M.M.(1994),High Brightness 100lm/W 45W CREE COB LED Track Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[95g 3D Tooth Stain Remover Whitelock Toothpaste]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control. Analytical Biochemistry212,436-445[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Laser Customized Telescopic Steel Cover]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2-25mm Co-Extruded Highlight PVC Foam Board]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Precision Horizontal Conventional Large Gear Hobbing Machine Hobber Y31100c]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Household Use Food Packing 8011 Aluminum Foil]

Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition]

Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection]

Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mu Optical Fiber Connectors for Cable Assembly, Part I.Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dubai Products Rectified Tile for Wall]

Best Price 201 304 Stainless Steel Coils, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),National Day Holiday Football Bravo Hand Weaving Flag.Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, 175-184[Wholesale Promotion 90 Degree Shower Door Glass Partition Brace Partition Accessories Shower Door Glass Clips]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[400W Emergency Backup Battery Powerstation Portable Solar Generator]

Whitaker,J.R.(1963),New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function. Analytical Chemistry35(12), 1950-1953[Car Interior Accessories Customized Car Seat Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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