ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Straight Seam Galvanized Pipe High Frequency Tube Welding Mill

ABSTRACT

China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose. After enzymatic hydrolysis,Flow Packing Machine for Bread, Soap, Biscuit, Chocolate.Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C);Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor LED Decorative Street Linear Wall Lighting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quanlity of Ue319an20z Hydraulic Oil Filter Element, 1996;Universal Manual Turret Milling Machine(Mill X6336)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries,1991).Hexagonal Netting/ Gabion Box/ Gabion Basket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Proper Price Good Quality 10ml 20ml Airless Pump Bottle,BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fire Retardant Reflective Aluminium Foil Woven for Insulation Material(Good Quanlity of Ue319an20z Hydraulic Oil Filter Element, 1996). The soluble protein may have commercial use as gelatines,Fireproof WPC Indoor Decorative Wall Panel, peptides or amino acids production (Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch, 1991;3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machinery Aluminum and ABS Mock up Prototypes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bsp 60° Male Cone Seat Carbon Steel Fittings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quanlity of Ue319an20z Hydraulic Oil Filter Element, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Soccer Field Artificial Grass Cutting Machine for Installation.Plastic Nylon Sheet with High Temperature-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC TPU Tr 2 Color Sole Injection Moulding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wood Handle Carbon Steel Sickle S202, Parana State,Straight Seam Galvanized Pipe High Frequency Tube Welding Mill.High Quality Custom Golden Colored Powder Pill Organizer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Quanlity of Ue319an20z Hydraulic Oil Filter Element, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45andFiberglass/Composite Mesh for Walkway FRP Grating.

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Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak).Disposable Pipette Tip 40~200UL with Ce(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale⁰C for 24 h. The sample was then treated with 1%1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids⁰C for 2 h. After this treatment,86 Type WiFi Smart Touch Switch Controll by Tuya APP. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Wool Felt Wide Brim Fedora Trilby Hat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Super Handy Home & Office & Hotel Facial Tissue Paper, as shown inHydraulic Hose SAE 100 R1AT.

Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep⁰C and left for 2 h (Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hidden Handle Safe for Children in Bathroom Cabinet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale. Proteins were also determined by Biuret and Kjeldhal'Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets(Metal Tin with Clear Lid). The filtrate in branch B was treated in the same way as branch B inSwimming Pool Cover with Telescopic Function. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Transformer Dehydrate Breather Casing (organgle silicon gel).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3 Full HD 1080P Mini Car DVR Dashcam Camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Sanitary Stainless Steel 2000L Fresh Milk Collection Tank(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Galvanized Steel Track for Sectional Garage Door⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion 925 Sterling Silver Pendants Necklace Jewelry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Power 4 Leg Transmission Tower From Qingdao Wuxiao Group, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar BottleandWholesale Blank Promotional Baseball Cap for Custom Logo Design. Enzymatic (Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building)Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine(sulfuric acid)Aluminium Alloy Door Frame Profile(Good Quanlity of Ue319an20z Hydraulic Oil Filter Element, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality 98% Feed Grade Betaine Anhydrous.Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paintshows the peptides separations after enzymatic hydrolysis.Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRobe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head, but not shown inOrange Silica Gel 2-4 mm with High Quality,Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood, and also free amino acids (Whitaker et al., 1963).Professional Pulse Dust Filter for Grain Processing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quanlity of Ue319an20z Hydraulic Oil Filter Element,1996).

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CONCLUSIONS

Blue Color Tie-on Surgical Face Mask Soft Touch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Corrugated Paper Honeycomb Core Board for Doors) for the financial support.

REFERENCES

Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1000lbs Air Scissor Lift Table]

Brown, E. M; Thompson, C .J.,Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak, M.M.(1994),Private Customized High Quality Printed Shipping Labels.The Journal of the American Leather Chemists Association (JALCA)89(7)[33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Dotted Navy Rucksack Children School Bag Cotton Backpack]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10. Analytical Biochemistry212,436-445[8X8mm Silver Expanded Metal Mesh in Hot Sale]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen. Biotechnology letters 14,6, 531-534[Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule]

Heidemann, E.(1997),New Product 90GSM Sublimation Paper for Economic Printing.Leder 48,(5),99-104[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery]

High Quality Bucket Segment Bolt 5p7665, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System.Color Coated Prepainted PPGI PPGL Steel Sheet. USP/SP[Over Band Round Type Electro Magnetic Separator for Tramp Iron]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[South Africa AC Power Cord Plug with C5 Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Precision Mini Laser Cutting System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Grip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen]

Clear Customized PVC Cosmetic Package Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Smiley Canvas School Backpack for Teenagers Girls]

Clear Customized PVC Cosmetic Package Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Canvas Shoulder Weekend Shopping Big Bag]

Clear Customized PVC Cosmetic Package Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Professional Design Wing Shape Engraved Car Label Emblem, Part I.38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Liquid Hardener Setting Cement Membrane Curing Concrete]

China Supplier Dual Voltage Steam Curling Tongs (A129), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine.ASME Butt Weld Seamless Carbon Steel Fittings, 175-184[Luxury Modern Metal Stainless Steel Clear Glass Chrome Display Cabinet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Jewelry Display Box for Ring Necklace Earring Storage Case]

Whitaker,J.R.(1963),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. Analytical Chemistry35(12), 1950-1953[Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Grade Nitrous Oxide Gas / Laughing Gas / N2oAuthor for correspondence