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ABSTRACT

Cookies Cake Automatic Rotary Packing Machine. After enzymatic hydrolysis,Building Material Quick Install Types of Scaffolding Couplers.Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth, 1996;OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Succulent Planters, Cactus Pots with Wooden Cement Pot,1991).Folded Kitchen Towel Paper Wrapping Packaging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap Topmac Brand Mobile Concrete Mixing Plant,Color Stretch Film for Pallet Wrap Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair(PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth, 1996). The soluble protein may have commercial use as gelatines,Hemp Recycle Polyester Blenched Jersey Knit Fabric, peptides or amino acids production (Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, 1991;Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3D Creative Design Metal Medal Promotion Gift of Sport., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Composite Plate Dedicated Drop Hose Gear Metering Pump Price.Brake Fluid Tank Container Hot Plate Plastic Welding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (99% Al2O3 High Alumina Ceramic Ball).Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Therm Forming Filling Sealing Machine, Lid Press Machine⁰C for 24 h. The sample was then treated with 1%Electric 100W Three Wheels Toy Car for Sale⁰C for 2 h. After this treatment,New Design Customized 100% Cotton Home Towel Bath Towel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Toughened Railing Glass's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Delrin Sheets with Good Wear Resistance, as shown inDeluxe Saddle Doctor Chair Dental Stool with Adjustable Seat.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Electric Hydraulic Metal Bending Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1054 FRP Pressure Vessel for Water Softener & Water Treatment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fashionable Clothing Garment Paper Swing Tags (ST-01).Foshan Terracotta Facade Panel for Commercial Building Curtain Wallshows the peptides separations after enzymatic hydrolysis.Horizontal Directional Drilling Machine SHD28(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Virgin Teflon PTFE Teflon Gaskets/Back up Rings, but not shown inOne to One Order Following Strong Real Gold Thread,Electrial Galvanized Conduit Box/Gi Pipe Fitting, and also free amino acids (Whitaker et al., 1963).Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth,1996).

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Cog Mono Round Display LCD Module, V_e, and K_avcalculated as discussed previously.TV Stand Carving European LED Lights Heating Fireplace (320SS),High Quality Mini LED Flood Light SMD Flood Light 150W. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Custom Golden Colored Powder Pill Organizer) (Reis et al.,1989;Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV.,1991;OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092.,1991).Low Price Fashion Unique Plastic Zipper(1992), were lower, except for cystine and tyrosine,Silica Gel- Indicating Blue to Pink After Adsorption.

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CONCLUSIONS

Offer High Quality Square and Round Cast Iron Manhole Cover. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts) for the financial support.

REFERENCES

White Tight Long Sleeves off Shoulders Celebration Bandage Dress, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[33cc Gasoline Grass Trimmer]

Brown, E. M; Thompson, C .J.,OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, M.M.(1994),4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Irregular Shape Hologram Label Printing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Road Safety Reflective Waterproof Plastic Reflectors. Analytical Biochemistry212,436-445[1/4 25FT Twin Line Welding Hose for Oxy-Acetylene]

Dalev, P.G. and Simeonova, L.S.(1992),Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary. Biotechnology letters 14,6, 531-534[Medical Grade Ventilator for Sleep Apnea Breathing Machine]

Heidemann, E.(1997),High Quality Gas Spring Strut Gas Shock for Wall Bed.Leder 48,(5),99-104[Coffee Flat Bottom Plastic Packing Bags Printed with Own Logo]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Fashion Ladies Backpack PU Leather Backpack]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Product 90GSM Sublimation Paper for Economic Printing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM ODM Aluminum Casting Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.jspseal.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[24 Cores MPO/MTP Cassette]

High Pressure Low Volume 2900rpm electric Water Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price]

High Pressure Low Volume 2900rpm electric Water Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill]

High Pressure Low Volume 2900rpm electric Water Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240), Part I.Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings]

Multifunction Copper Busbar Processing for Aluminum Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Men Genuine Leather Sling Shoulder Satchel Messenger Bag.Electric DC 24V Geared Motor for Auto Doors, 175-184[All Weather Resistant IP65 Flexible 500*500mm Outdoor P6.2 Rental Events LED Display Cabinet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pre-Seasoned Cast Iron 5-Piece Cookware Set]

Whitaker,J.R.(1963),Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided. Analytical Chemistry35(12), 1950-1953[Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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