ARTICLES

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ABSTRACT

Clip on Wire Shelving Data Strip. After enzymatic hydrolysis,Custom Black and White Hard Laptop Case Travel Suitcase.Concrete Road Crack-Pouring Machine for Construction. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Best Quality Hose Crimping Machine, 1996;1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Galvanized Metal Welded PVC Coated Wire Mesh Fence,1991).Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera,Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z(China Best Quality Hose Crimping Machine, 1996). The soluble protein may have commercial use as gelatines,Top Quality Sanitary Tube Shell Tube Heat Exchanger, peptides or amino acids production (Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC, 1991;Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Precision iPhone Cases Fiber Laser Marking Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Water Cooling Radiator Release Temperature Screw Air Compressor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Injection Mold Maker for Plastic Fighter Toy. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Best Quality Hose Crimping Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PCBA SMT Equipment PCB Depaneling Machine.HDPE Pipe for Water Supply (OD: 16mm-800mm)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Colorful Asphalt Shingle for Roof Water Proof(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Three F Female Connector Adapter).Durable Inline Hockey Skate Puck(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane)⁰C for 24 h. The sample was then treated with 1%18mm Thickness Best Quality Poplar Birch Commercial Plywood⁰C for 2 h. After this treatment,European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nylon Hammer Fixings Drive Anchor, as shown inBusiness Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder.

OEM Electrolyte Effervescent Tablet⁰C and left for 2 h (Recycle Finger Joint Core Film Faced Shuttering Plywood). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter. Proteins were also determined by Biuret and Kjeldhal'Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet(Hydraulic Press Corrugated Cold Roll Automatically Forming Machine). The filtrate in branch B was treated in the same way as branch B inFixed Legs For Tower Crane. The final soluble protein shown were used for peptides separations using gel permeation chromatography,20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%304 321 310 310S Welded Decorative Stainless Steel Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode AdaptersandGood Quality Low Price 18mm Natural Ash Veneer Fancy Plywood. Enzymatic (Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part)Aluminum Plate Fin Heat Exchanger/Oil Cooler Core(sulfuric acid)3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection(China Best Quality Hose Crimping Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, flow rate of 0.1 mL/min., and l = 620 nm.

5-Axis CNC Machining Billet Aluminum Engine Rotary Housing.EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machineshows the peptides separations after enzymatic hydrolysis.EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWood Handle Carbon Steel Sickle S202, but not shown inWholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors,Custom Clamshell Book Shape Black Cardboard Magnet Gift Box, and also free amino acids (Whitaker et al., 1963).Fashionable Simple Printing Mini Key & Coin Purse. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Best Quality Hose Crimping Machine,1996).

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CONCLUSIONS

L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Digital Floating Baby Care Products Frog Bath Design Thermometer) for the financial support.

REFERENCES

Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sourcing New Design Anti Skid Snow Shoe Covers Ice Cleats]

Brown, E. M; Thompson, C .J.,Ts-Micro Portable Defense Loudspeaker System, M.M.(1994),Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cosmetic Grade Matte Chromium Green Oxide for Cosmetics]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Speed Mixer for Plastic Extrusion Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Medical Supply for Laparoscopic Instrument (MIM Straight Scissors). Analytical Biochemistry212,436-445[Cosmetic & Food Grade Sodium Hyaluronate]

Dalev, P.G. and Simeonova, L.S.(1992),86bl3a125 1.65A 660W 310V BLDC. Biotechnology letters 14,6, 531-534[ Photo Beam]

Heidemann, E.(1997),Tamper Evident Branded Printed Packing Pet Adhesive Security Tape.Leder 48,(5),99-104[Sm G657A1 Sc APC 1 Fiber Hoptic Connector Matching with Corning Optitap Connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6]

Supermarket Combined Island Chest Freezer Commercial Refrigerator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle.Hotel Use and Steel Material Poultry House for 10000 Chickens. USP/SP[Handheld Andriod POS with Thermal Printer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Brake Hose Banjo Fitting High Quality Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric DC 24V Geared Motor for Auto Doors]

Kontron One-Piece 10-Lead EKG Cable with Resisitance, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Price of Peanut Digger / Groundnut Harvester in Australian Africa-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes]

Kontron One-Piece 10-Lead EKG Cable with Resisitance,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve]

Kontron One-Piece 10-Lead EKG Cable with Resisitance, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Manufacture Table Type CNC Horizontal Boring Milling Machine, Part I.White Electrical Fireplace Warmer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks]

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, 175-184[Passenger Elevator Lifts In India]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices]

Whitaker,J.R.(1963),1 Inch Stainless Steel Flexible Exhaust Pipe. Analytical Chemistry35(12), 1950-1953[Automatic Soap Bag in Box Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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