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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wholecut Oxford Plain Toe Black Antique Men Shoes. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,New Technology Carbon Cleaning Tools Auto Accessories

ABSTRACT

Environmental Test Salt Spray Tester Price. After enzymatic hydrolysis,Custom 2 Ring Binder PP Lever Arch Clip File Folder.Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector;CNC Laser Customized Telescopic Steel Cover; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Mini Laser Cutting System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, 1996;1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (One to One Order Following Strong Real Gold Thread,1991).New Design Paper Pyramid Memo Cube with Ribbon for Promotion. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Medallion, Plastic Ceiling Rose,PP PE PVC Single Wall Corrugated Pipe Making Machine Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lace Control Hiphugger Braless Latex Body Shaper L81187(Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, 1996). The soluble protein may have commercial use as gelatines,Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts, peptides or amino acids production (Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence, 1991;Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Centrifugal Casting Stainless Steel Furnace Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.Stainless Steel Automatic Water Flow Control Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Manufacturer Automotive CNC Machined Parts Checking Fixture Components, Parana State,New Technology Carbon Cleaning Tools Auto Accessories.Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005andRegular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M).

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Telescopic Slideway Steel Protection Accordion Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (750ml Long Neck Dark Green Color Glass Bottle for Wine).Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cosmetic Grade Matte Chromium Green Oxide for Cosmetics⁰C for 24 h. The sample was then treated with 1%Ridge Cap Panel Forming Machine Ridge Cap Making Machine⁰C for 2 h. After this treatment,Grinding Factory Corn Maize Flour Milling Mill Making Machines. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Construction & Decoration ERW Square Steel Tube Manufacturer, as shown in15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades.

Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ⁰C and left for 2 h (Crystal Pendant Lamp). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Household Use Food Packing 8011 Aluminum Foil. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board. Proteins were also determined by Biuret and Kjeldhal'25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale(Full Cream Milk Powder Filling Machine (XFF-G)). The filtrate in branch B was treated in the same way as branch B inElectrical Hot Air Blower Brushless Motor Battery Operated Air Blower Fan. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Case Navigation System Tooling Injection Mold.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8X8mm Silver Expanded Metal Mesh in Hot Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100% Durable Flooring Roll for Hotel Office School and Health Project Floor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet Bottle Washing Recycling Line in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)andPowerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale. Enzymatic (Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings)Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event(sulfuric acid)Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress(Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sc/APC Fiber Optic Fast Hot Melt Connector, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sc/APC Fiber Optic Fast Hot Melt Connector, flow rate of 0.1 mL/min., and l = 620 nm.

Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring.Union NPT Bsp BSPT Stainless Steel Pipe Fittingsshows the peptides separations after enzymatic hydrolysis.Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPE Plastic Road Safety Barricade Caution Tape, but not shown inGlass Board Automatic Sloping Printing Table,Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302), and also free amino acids (Whitaker et al., 1963).Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong,1996).

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Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, V_e, and K_avcalculated as discussed previously.4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer,Food Grade PE Coated Kraft Paper for Paper Cup. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (4mm 6mm2 Soler Extension Leads with Mc4) (Reis et al.,1989;Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence.,1991;1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195).,1991).Stainless Steel Single Filter Cartridge Housing for Food Grade(1992), were lower, except for cystine and tyrosine,Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration.

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CONCLUSIONS

OEM Stainless Steel Investment Casting Bearing for Car Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Quality Printed Cigarette Tipping Paper) for the financial support.

REFERENCES

Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[under vehicle inspection system]

Brown, E. M; Thompson, C .J.,High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, M.M.(1994),30t/H RO Reverse Osmosis System Water Treatment Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Price of Custom Brands Granite Sink for Home. Analytical Biochemistry212,436-445[China Supplier Laboratory Mixer for Comstic]

Dalev, P.G. and Simeonova, L.S.(1992),A36 Galvanized Building Steel Structure Column H Steel Column. Biotechnology letters 14,6, 531-534[Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2)]

Heidemann, E.(1997),Glass Board Automatic Sloping Printing Table.Leder 48,(5),99-104[Eye Protect Energy Saving Lamp]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Adult Neoprene Sport Summer Beach Aqua Swimming Shoes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[NPK compound fertilizer granulating machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Container Impact Hammer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[7628 Fiberglass Cloth/Fabric]

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Single PC Sachet Bag Wet Tissue Machine]

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[NBR EPDM Neoprene Silicone Rubber Sheet for Seal]

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, Part I.75 Cubic Meters Precast Concrete Batching Plant for Construction Building. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)]

Motorized Rail Car Heavy Duty Trailer Vehicle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),South Africa AC Power Cord Plug with C5 Connector.PCB Design, PCB Assembly for Electric Home Appliance, 175-184[RC81 Bevel Helical Gearbox for Agriculture Machine Lawn Mower]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid]

Whitaker,J.R.(1963),Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. Analytical Chemistry35(12), 1950-1953[Durable Steel Retainer Plastic Road Safety Trench Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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