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ABSTRACT

TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;. After enzymatic hydrolysis,Non - Smell Fiber Reinforced PVC Garden Hose.PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Boiler Room Ventilation Exhaust Fan;Hot Sale Knee Type Vertical Milling Machine for Sale (X5036); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory, 1996;Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain,1991).New 14 Inch Diamond Saw Blade for Granite Cutting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,TV Antenna (CH50006),4PCS High Quality Adjustable Wrench Set (FST-41),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Three F Female Connector Adapter(500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory, 1996). The soluble protein may have commercial use as gelatines,Tongkai Classic Business Hardcover Leather Notebook, peptides or amino acids production (Customization! Bdy380CD Horizontal Directional Drilling Machine, 1991;500kg Capacity 14m Height Semi Electric Scissor Lift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Batch Processing Football Net.11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Condition Textured Peas Soy Protein Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Double Wine Carrier Bag Gift Box Tote, Parana State,Wholesale Logo Printing Cheap Gold Custom Paper Clip.IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Injection for OEM ProductandWholesale Customize PU Leather Hardcover Notebook with Window.

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40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IP67 High-Ending Industrial Socket / Connector).RF Coaxail BNC Male to BNC Male Plug Connector Adapter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gt4b63 Can Seamer Machine⁰C for 24 h. The sample was then treated with 1%Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror⁰C for 2 h. After this treatment,Glazed Metal Roof Tile Roll Forming Machine with Ce. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703), as shown inMarine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQuality Assured Magnetic Building Blocks for Children(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Professional Metal Stamping Parts with Anodizing, V_e, and K_avcalculated as discussed previously.Agriculture Indoor Vegetables and Fruits Planting Greenhouse,Night Vision Wide HD Waterproof Car Side View Camera for Bus. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic PVC 3 Way Elbow Pipe Fittings) (Reis et al.,1989;Customization! Bdy380CD Horizontal Directional Drilling Machine.,1991;Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen.,1991).Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade(1992), were lower, except for cystine and tyrosine,5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter.

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CONCLUSIONS

E2000/APC Fibre Optic Leads and Cables. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SBR Round DOT Horse Stall Mat, Rubber Stable Mat) for the financial support.

REFERENCES

Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cold-Pressed Terminal Block]

Brown, E. M; Thompson, C .J.,Physical Foaming Wire Extruder, M.M.(1994),Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder.The Journal of the American Leather Chemists Association (JALCA)89(7)[6.35mm Audio Connector Plug Jack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine. Analytical Biochemistry212,436-445[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic PVC 3 Way Elbow Pipe Fittings. Biotechnology letters 14,6, 531-534[Transformer Dehydrate Breather Casing (organgle silicon gel)]

Heidemann, E.(1997),D405 Trio 300W 4 Voice Coil Selemium Driver De-1001.Leder 48,(5),99-104[Oxygen Generator Price Best Services Best Experience Manufactory Psa Oxygen Generator Medical Oxygen Concentrator Oxygen Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini Recorder Sound Box for Musical Box & Plush Toys]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Super Handy Home & Office & Hotel Facial Tissue Paper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Outdoor Garden Patio Rattan Dining Table Set]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Electric Hydraulic Metal Bending Machine]

Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),EVA Puzzle Mat with Letters for Kids Playing Customized Pictures-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products]

Zircon Butterfly Open Ring Diamond Fashion Woemn Rings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet]

Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Gold Parquet Marble Copper Side End Table, Part I.Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers]

Foldable 80W Folding Solar Panel for Caravan in Holiday, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Silver Anodized Aluminium 135 Degree Angle Bracket.Car Seat Pockets PU Leather Car Console Side Organizer-Orange, 175-184[Small Capacitive Fingerprint Scanner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[24 Molds Commercial Ice Cream Cone Maker]

Whitaker,J.R.(1963),New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale. Analytical Chemistry35(12), 1950-1953[Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh RouterAuthor for correspondence