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ABSTRACT

Casting PE Film with Ash for Adult Diaper Back Sheet. After enzymatic hydrolysis,Supra Brand Laundry Liquid Baby Formula.Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, 1996;Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cookies Cake Automatic Rotary Packing Machine,1991).Building Material Quick Install Types of Scaffolding Couplers. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury,Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft(6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, 1996). The soluble protein may have commercial use as gelatines,Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt, peptides or amino acids production (PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth, 1991;OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mini Succulent Planters, Cactus Pots with Wooden Cement Pot., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Folded Kitchen Towel Paper Wrapping Packaging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Topmac Brand Mobile Concrete Mixing Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Color Stretch Film for Pallet Wrap Use.Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hemp Recycle Polyester Blenched Jersey Knit Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe, Parana State,Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles.Composite Plate Dedicated Drop Hose Gear Metering Pump Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly SetandOEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch.

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Brake Fluid Tank Container Hot Plate Plastic Welding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm).6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish⁰C for 24 h. The sample was then treated with 1%Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding⁰C for 2 h. After this treatment,High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Puffed Core Filling Sanck Production Machine with Ce From China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting, as shown inElectronic Muscle Stimulator EMS Machine for Slimming.

Cummins Fuel Injector Kit (3076736 3095023)⁰C and left for 2 h (Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch. Proteins were also determined by Biuret and Kjeldhal'Plastic Therm Forming Filling Sealing Machine, Lid Press Machine( 15mm). The filtrate in branch B was treated in the same way as branch B inBlack Prarlescent Masterbatch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric 100W Three Wheels Toy Car for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design Customized 100% Cotton Home Towel Bath Towel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avToughened Railing Glass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High quality Brass Forged Gate Valve with Casting Iron Handle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3D Creative Design Metal Medal Promotion Gift of Sport) for the financial support.

REFERENCES

Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12 cordas de guitarra]

Brown, E. M; Thompson, C .J.,High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture, M.M.(1994),Cog Mono Round Display LCD Module.The Journal of the American Leather Chemists Association (JALCA)89(7)[A616 Axe with Wooden Handle Series]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-TV Stand Carving European LED Lights Heating Fireplace (320SS). Analytical Biochemistry212,436-445[4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Mini LED Flood Light SMD Flood Light 150W. Biotechnology letters 14,6, 531-534[Factory Wholesale Reach Standard Home and Office Furniture Fitting]

Heidemann, E.(1997),Low Price Fashion Unique Plastic Zipper.Leder 48,(5),99-104[Embossed Foil Aluminium Foil Jumbo Roll]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Tight Long Sleeves off Shoulders Celebration Bandage Dress: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Drum Rotator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Safety Reflective Underground PVC Warning Tape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Lid Press Machine]

OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets]

OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Unfolding Grocery Push Carts]

OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Road Safety Reflective Waterproof Plastic Reflectors, Part I.Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bwb-306 Custom Printing Outdoor Sports Wristband]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply]

Whitaker,J.R.(1963),OEM ODM Aluminum Casting Parts. Analytical Chemistry35(12), 1950-1953[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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