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ABSTRACT

Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport. After enzymatic hydrolysis,Bitumen Waterproof Membrane Minerla Surface Waterproof Material.M10 3D Solid Non-Woven Folded Mask Body Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pfc Inductors with Adjustable Dimension and Wire Diameter;Automatic Permanent Magnetic Lifter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Large Diameter Aluminum Pipe 3004, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light, 1996;Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine,1991).Wholesale Logo Printing Cheap Gold Custom Paper Clip. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;,Non - Smell Fiber Reinforced PVC Garden Hose,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet(Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light, 1996). The soluble protein may have commercial use as gelatines,Boiler Room Ventilation Exhaust Fan, peptides or amino acids production (Hot Sale Knee Type Vertical Milling Machine for Sale (X5036), 1991;Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New 14 Inch Diamond Saw Blade for Granite Cutting.TV Antenna (CH50006)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4PCS High Quality Adjustable Wrench Set (FST-41)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Price Long Life Portable Peltier Air Cooler).Small Batch Processing Football Net(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router⁰C for 24 h. The sample was then treated with 1%New Condition Textured Peas Soy Protein Making Machine⁰C for 2 h. After this treatment,Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SBR Round DOT Horse Stall Mat, Rubber Stable Mat, as shown inASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross.

Customized Frame Mount Highway Pegs Enclosure Parts⁰C and left for 2 h (Cavitation Vacuum RF Lipo Laser Slimming Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Double Wine Carrier Bag Gift Box Tote. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket. Proteins were also determined by Biuret and Kjeldhal'40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls(Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch). The filtrate in branch B was treated in the same way as branch B inNt Build 492 Chloride Migration Coefficient Tester. The final soluble protein shown were used for peptides separations using gel permeation chromatography,RF Coaxail BNC Male to BNC Male Plug Connector Adapter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Gt4b63 Can Seamer Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIlluminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Quality Assured Magnetic Building Blocks for Children.Hotel Use and Steel Material Poultry House for 10000 Chickensshows the peptides separations after enzymatic hydrolysis.Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate, but not shown inOEM Ductile Gully Iron Guard Drainage Manhole Cover,Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, and also free amino acids (Whitaker et al., 1963).Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light,1996).

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PVD Vacuum Metal Coating Machine for Stainless SteelandPipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safeshow the gel chromatographic separation of soluble protein with Sephadex G-Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint.Cummins L10 Engine Piston Cooling Nozzle 3070218, and another peak at 45 mL not shown inChina Xr150L/Pulsar 180 Engine Parts Cylinder Kit, for peptides lower than 3000 Da.Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card(Condell et al.,1993) .

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Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, V_e, and K_avcalculated as discussed previously.SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use,99% Al2O3 High Alumina Ceramic Ball. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile) (Reis et al.,1989;Hot Sale Knee Type Vertical Milling Machine for Sale (X5036).,1991;Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater.,1991).Factory Price Long Life Portable Peltier Air Cooler(1992), were lower, except for cystine and tyrosine,8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club.

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CONCLUSIONS

Hand Tools 9PCS Hex Key Set CRV Allen Wrench. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customization! Bdy380CD Horizontal Directional Drilling Machine) for the financial support.

REFERENCES

UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Cbd Oil Vape Pen]

Brown, E. M; Thompson, C .J.,Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply, M.M.(1994),Handheld Andriod POS with Thermal Printer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fence Mesh Welding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel French Earring Hook for Jewelry. Analytical Biochemistry212,436-445[Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches]

Dalev, P.G. and Simeonova, L.S.(1992),Professional Metal Stamping Parts with Anodizing. Biotechnology letters 14,6, 531-534[Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box]

Heidemann, E.(1997),Agriculture Indoor Vegetables and Fruits Planting Greenhouse.Leder 48,(5),99-104[Personality Cat Eye Sunglasses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Mobile Phone Shop Display Furniture Interior Design]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Paper Coated Office Furniture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Support Intel Core3 I7/I5/I3 Processors]

E2000/APC Fibre Optic Leads and Cables, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts]

E2000/APC Fibre Optic Leads and Cables,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use]

E2000/APC Fibre Optic Leads and Cables, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Physical Foaming Wire Extruder, Part I.Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Power Solar Inverter with Full Power 3000W Converter]

10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic PVC 3 Way Elbow Pipe Fittings.D405 Trio 300W 4 Voice Coil Selemium Driver De-1001, 175-184[Promotion Backpack Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double Jacketed Cooking Kettle Electric Jacket Boiler]

Whitaker,J.R.(1963),150 micron steel mesh filter magnetic hydraulic filter cartridge. Analytical Chemistry35(12), 1950-1953[Fashion Party Decoration Classic Wine Glass Accessories Jewelry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

One Compartment Commercial Sink Without DrainboardAuthor for correspondence