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ABSTRACT

Telescopic Slideway Steel Protection Accordion Cover. After enzymatic hydrolysis,Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes.Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ridge Cap Panel Forming Machine Ridge Cap Making Machine;Grinding Factory Corn Maize Flour Milling Mill Making Machines; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Construction & Decoration ERW Square Steel Tube Manufacturer, 1996;Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Household Use Food Packing 8011 Aluminum Foil,1991).Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale,Plastic Case Navigation System Tooling Injection Mold,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set(Construction & Decoration ERW Square Steel Tube Manufacturer, 1996). The soluble protein may have commercial use as gelatines,8X8mm Silver Expanded Metal Mesh in Hot Sale, peptides or amino acids production (Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst, 1991;100% Durable Flooring Roll for Hotel Office School and Health Project Floor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pet Bottle Washing Recycling Line in China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Construction & Decoration ERW Square Steel Tube Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings.Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium, Parana State,Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting.Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Construction & Decoration ERW Square Steel Tube Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectrochemical Polishing Tungsten Evaporation MaterialsandHigh Quality 20PCS Colorful Makeup Brush Set.

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SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5).Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags⁰C for 24 h. The sample was then treated with 1%4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer⁰C for 2 h. After this treatment,Food Grade PE Coated Kraft Paper for Paper Cup. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Single Filter Cartridge Housing for Food Grade's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, as shown inToughened Railing Glass.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%A36 Galvanized Building Steel Structure Column H Steel Column. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlass Board Automatic Sloping Printing Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, pH 3.25., mobile phase B: buffer solution of 0.01 mol/H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

1.5tons Mini Site Dumper SD15. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates) for the financial support.

REFERENCES

1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Industrial Steel Latex Pump]

Brown, E. M; Thompson, C .J.,Stainless Steel Self Recovery PVC High Speed Roller Shutter, M.M.(1994),OEM Ductile Gully Iron Guard Drainage Manhole Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[CF8m Air Actuated Valve Dn15]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Precision Rail Slider 20mm Diameter PMI Linear Guideway. Analytical Biochemistry212,436-445[Integrated Hydraulic Steering Control Units (BZZ1F SCU)]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Price Cat Scratching Trees Cat Tree Luxury. Biotechnology letters 14,6, 531-534[10mm Gold Plated Stainless Steel Balls G100 Polish Ball]

Heidemann, E.(1997),China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.Leder 48,(5),99-104[Building Materials Drywall System Steel Keel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Profile Sp-Level Linear Rail for 3D Printer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Geotextile Beg Used for Beach Restoration Geobag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.sinoriseind.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch]

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Paulownia Wood Board Wood Drying Kilns for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ss304 Sanitary Welded Butterfly Valve]

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[500kg Capacity 14m Height Semi Electric Scissor Lift]

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Seamless Boxer Brief for Men, Part I.Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)]

Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies.Lime Dewatering Filter Press Cloth Filter, 175-184[Bamboo Plywood 3mm Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine]

Whitaker,J.R.(1963),150ml Round Aromatherapy Diffuser Glass Bottle. Analytical Chemistry35(12), 1950-1953[White UTP Cat5e UK Type Tooless RJ45 Keystone Jack]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Double Bowl Undermount for Stainless Steel Kitchen SinkAuthor for correspondence