ARTICLES

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ABSTRACT

Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. After enzymatic hydrolysis,Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids.Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair;Single Block Vertical Wire Drawing Machine for Screw and Bolt Making; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Electric PCB Circuit Board with High Quality, 1996;OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anodized Perforated 3D Facade Windows Screen for Commercial Building,1991).Full Cream Milk Powder Filling Machine (XFF-G). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.,Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale(China Electric PCB Circuit Board with High Quality, 1996). The soluble protein may have commercial use as gelatines,Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, peptides or amino acids production (Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment, 1991;PVC Car Seat Belt Shoulder Protector 80438, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Full Automatic Machine for Making Wood Pellets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 20PCS Colorful Makeup Brush Set. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Electric PCB Circuit Board with High Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy.PE Plastic Road Safety Barricade Caution Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Screw Barrel for PVC PPR Pipe Extrusion(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable, Parana State,Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression.95g 3D Tooth Stain Remover Whitelock Toothpaste, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Electric PCB Circuit Board with High Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGeotextile Beg Used for Beach Restoration Geobagand50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch.

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Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500kg Capacity 14m Height Semi Electric Scissor Lift).Al/Pet/Al Double Sides for Aluminium Flexible Air Duct(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%European Standards 100A 24V Electrical Relay for Green Energy⁰C for 24 h. The sample was then treated with 1%Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal⁰C for 2 h. After this treatment,Canvas Shoulder Weekend Shopping Big Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Visuable Reflective Tape for Vehicle Road Safety's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Made in China Aluminum Extrusion Window and Door Profile, as shown inH2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDurable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Experienced Construction Steel Structure Building Design⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Duty 16000L High Pressure Sewer Cleaning Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Items Gh/Genatropin with Naked Vial.High Speed Automatic Slitting Machine for Plastic Filmshows the peptides separations after enzymatic hydrolysis.Custom Size Gel Nail Polish Set Boxes with Sponge(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCe Approved Bowrider Leisure Boat for 6-8 People, but not shown inTop Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1,High Precision Ce Radial Drill Machine Z3040, and also free amino acids (Whitaker et al., 1963).Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Electric PCB Circuit Board with High Quality,1996).

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Hot-Sale Products GRP Tower for New Energy Industry, V_e, and K_avcalculated as discussed previously.Fgg 2b 304 Metal Circular Push Pull Connector,Thick Wall Large Diameter Aluminium Tube Manufacture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ANSI B16.5 Stainless Steel SS316 Forging Blind Flange) (Reis et al.,1989;Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment.,1991;OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts.,1991).Portable Hydraulic Power Pack 12V Hydraulic Power Unit(1992), were lower, except for cystine and tyrosine,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.

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CONCLUSIONS

52cc Petrol Brush Cutter for DIY Garden Care. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor) for the financial support.

REFERENCES

Anti-Theft EAS Hard Tag Tag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVDF 1000mm Z60 Prepainted Steel Coil for Power Plant]

Brown, E. M; Thompson, C .J.,Foldable Silicone Vegtable Fruit Colander Strainer, M.M.(1994),Hans Custom Manufactured Smoothness Zipper Waterproof.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D Inkjet Printing Waterproof Interior Kitchen Wall Tile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quartz Liquid Powder Feeding Torch. Analytical Biochemistry212,436-445[Large Capacity Food Processing Cooling Tower Conveyor Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail. Biotechnology letters 14,6, 531-534[Aluminum Lamp Body 90mm Cut out Square LED COB Downlights]

Heidemann, E.(1997),Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing.Leder 48,(5),99-104[Dermal Filler Gel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hardcover Bear′s Story Children Book Custom Printing]

CNC Aluminum Laser Milling Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outdoor P5mm Programmable Bus LED Display Sign.AGM Separator for AGM Battery. USP/SP[Mineral Water Bottle Mould Plastic Blowing Mold]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V 96LED Outdoor Lighting Solar Torch Light for Christmas]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electric Battery Flameless LED Tea Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Items Gh/Genatropin with Naked Vial]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Steel Beam Sizes Ceiling Furring Channel for Gypsum Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CE Certificated Wheel Loader (HQ912) with Agricultural Tire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Manufacture Table Type CNC Horizontal Boring Milling Machine]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Price 201 304 Stainless Steel Coils]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays, Part I.Cosmetic & Personal Care Grade Xanthan Gum. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pressure Pump for Waterjet Machine]

Professiona Ozone Generator for Swimming Pool, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Precision Plastic Mold Manufacturer in China.Fiberglass Animal Poultry Insulation Grain Tower, 175-184[Gear Operated Solid Wedge Non Rising Stem Gate Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot-Sale Products GRP Tower for New Energy Industry]

Whitaker,J.R.(1963),Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts. Analytical Chemistry35(12), 1950-1953[Healthy Grooming Kit for Caring Newborn Baby]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Composite Machining Phenolic Titanium Parts Products SupplierAuthor for correspondence